Unique Kinetic Properties of Phenol-Degrading
            <i>Variovorax</i>
            Strains Responsible for Efficient Trichloroethylene Degradation in a Chemostat Enrichment Culture

Futamata, Hiroyuki; Nagano, Yayoi; Watanabe, Kazuya; Hiraishi, Akira

doi:10.1128/aem.71.2.904-911.2005

Cited by 79 publications

(57 citation statements)

References 40 publications

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“…Recently, it was found that biological processes might be linked with some specific dominant microbial populations [11,20,28]. Therefore, some dominant bacterial species were assumed to play an important role in maintaining the physical structure of aerobic granules and degrading pollutants [15,17].…”

Section: Discussionmentioning

confidence: 99%

Properties of phenol-removal aerobic granules during normal operation and shock loading

Jiang

Maszenan

Zhao

et al. 2009

J Ind Microbiol Biotechnol

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The physical structure and activity of aerobic granules, and the succession of bacterial community within aerobic granules under constant operational conditions and shock loading were investigated in one sequencing batch reactor over ten months. While the maturation phase of the granulation process began on day 30, the structure of microbial community changed markedly until after three months of reactor operation under constant conditions with a loading rate of 1.5 g phenol L(-1) day(-1). A shock loading of 6.0 g phenol L(-1) day(-1) from days 182-192 led to divergence of bacterial community, an inhibition of the biomass activity, and a decrease in phenol removal rate in the reactor. However, phenol was still completely removed under this disturbance. After the shock loading, the mean sizes of aerobic granules increased, and the activity of the microbial population within the granules decreased, although there appeared highly resilient for the dominant bacterial community of aerobic granules which mainly included beta-Proteobacteria. Correlation analysis suggested that biomass concentration and biomass loading were significantly related to the community composition of aerobic granules during the whole operational period. The development of a relatively stable bacterial community in aerobic granules implied that those distinct dominant microbes in aerobic granules were favorably selected and proliferated under the operational conditions.

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Section: Discussionmentioning

confidence: 99%

Properties of phenol-removal aerobic granules during normal operation and shock loading

Jiang

Maszenan

Zhao

et al. 2009

J Ind Microbiol Biotechnol

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“…Most of these bacterial genera have been cited in the literature in relation to the biodegradation of different environmental pollutants, like polycyclic aromatic hydrocarbons (PAHs) [8,22,23,45], trichloroethylene [16], aniline [2], dichloromethane [25], alkylbenzenesulfonates [37], and various pesticides [3,4,21,31,33,39]. However, apart from Stenotrophomonas sp.…”

Section: Total and Culturable Bacterial Diversity In Tnt-contaminatedmentioning

confidence: 98%

Effect of 2,4,6-trinitrotoluene on soil bacterial communities

George

Eyers

Stenuit

et al. 2008

J Ind Microbiol Biotechnol

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To gain insight into the impact of 2,4,6-trinitrotoluene (TNT) on soil microbial communities, we characterized the bacterial community of several TNT-contaminated soils from two sites with different histories of contamination and concentrations of TNT. The amount of extracted DNA, the total cell counts and the number of CFU were lower in the TNT-contaminated soils. Analysis of soil bacterial diversity by DGGE showed a predominance of Pseudomonadaceae and Xanthomonadaceae in the TNT-contaminated soils, as well as the presence of Caulobacteraceae. CFU from TNT-contaminated soils were identified as Pseudomonadaceae, and, to a lesser extent, Caulobacteraceae. Finally, a pristine soil was spiked with different concentrations of TNT and the soil microcosms were incubated for 4 months. The amount of extracted DNA decreased in the microcosms with a high TNT concentration [1.4 and 28.5 g TNT/kg (dry wt) of soil] over the incubation period. After 7 days of incubation of these soil microcosms, there was already a clear shift of their original flora towards a community dominated by Pseudomonadaceae, Xanthomonadaceae, Comamonadaceae and Caulobacteraceae. These results indicate that TNT affects soil bacterial diversity by selecting a narrow range of bacterial species that belong mostly to Pseudomonadaceae and Xanthomonadaceae.

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“…After electrophoresis, the gel was stained with SYBR Green I (Molecular Probes) for 30 min following the manufacturer's instructions. The nucleotide sequences of DGGE bands were determined as described previously (Futamata et al, 2005, Yoshida et al, 2005.…”

Section: Pcr-dggementioning

confidence: 99%

“…A number of laboratory studies have demonstrated that TCE is transformed cometabolically by aliphatic and aromatic hydrocarbondegrading bacteria under aerobic conditions (Ensley, 1991;Semprini, 1997;Futamata et al, 2005), while PCE is not transformed under aerobic conditions. However, PCE is reductively dechlorinated under anaerobic conditions by certain kinds of anaerobic bacteria (Smidt and de Vos, 2004;Futamata and Hiraishi, 2007).…”

Section: Introductionmentioning

confidence: 99%

Reductive dechlorination of chloroethenes by Dehalococcoides-containing cultures enriched from a polychlorinated-dioxin-contaminated microcosm

et al. 2007

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The reductive dechlorinating abilities for chloroethenes of seven enrichment cultures from polychlorinated-dioxin-dechlorinating microcosm were investigated using culture-independent and -dependent methods. These cultures were constructed and maintained with 1,2,3-trichlorobenzene (1,2,3-TCB) or fthalide as an electron acceptor and hydrogen as an electron donor. Denaturing gradient gel electrophoresis (DGGE) analysis of the amplified fragments targeting the 16S rRNA gene showed one or two major bands, whose nucleotide sequences were then analyzed and were found to suggest that Dehalococcoides was one of the dominant bacteria in all enrichment cultures. The nucleotide sequence data revealed that the identity of the major band was 100% identical to the 16S rRNA gene sequence of the Pinellas subgroup of the Dehalococcoides clusters, that is, strains CBDB1 and FL2. Genetic diagnosis targeting the pceA, tceA, bvcA, vcrA and reductive dehalogenase homologous (rdh) gene was performed to investigate the potential for reductive chloroethene dechlorination of cultures. The required length of PCR-amplified fragments was not observed, suggesting that these cultures are not capable of reductively dechlorinating chloroethenes. However, a culture-dependent test indicated that two cultures, TUT1903 and TUT1952, reductively dechlorinated tetrachloroethene (PCE) to trichloroethene (TCE), although not completely. While, TUT2260 and TUT2264 completely converted PCE to TCE and dichloroethenes, but not further. These results suggest that these TUT cultures might include a novel type of bacteria belonging to the Dehalococcoides group and that currently available information on both the 16S rRNA gene and rdh gene sequences is insufficient to definitively evaluate the potential abilities for reductive dechlorination.

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Unique Kinetic Properties of Phenol-Degrading Variovorax Strains Responsible for Efficient Trichloroethylene Degradation in a Chemostat Enrichment Culture

Cited by 79 publications

References 40 publications

Properties of phenol-removal aerobic granules during normal operation and shock loading

Properties of phenol-removal aerobic granules during normal operation and shock loading

Effect of 2,4,6-trinitrotoluene on soil bacterial communities

Reductive dechlorination of chloroethenes by Dehalococcoides-containing cultures enriched from a polychlorinated-dioxin-contaminated microcosm

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