Infrared (IR) emission features at 3. 3, 6.2, 7.7, 8.6 and 11.3 µm are generally attributed to IR fluorescence from (mainly) FUV pumped large Polycyclic Aromatic Hydrocarbon (PAH) molecules. As such, these features trace the FUV stellar flux and are thus a measure of star formation. We examined the IR spectral characteristics of Galactic massive star forming regions and of normal and starburst galaxies, as well as AGNs and ULIRGs. The goal of this study is to analyze if PAH features are a good qualitative and/or quantitative tracer of star formation and hence the application of PAH emission as a diagnostic tool in order to identify the dominant processes contributing to the infrared emission from Seyfert's and ULIRGs. We develop a new MIR/FIR diagnostic diagram based upon our Galactic sample and compare it to the diagnostic tools of Genzel et al. (1998) and(Laurent et al. 2000), with these diagnostic tools also applied to our Galactic sample. This MIR/FIR diagnostic is derived from the FIR normalized 6.2 µm PAH flux and the FIR normalized 6.2 µm continuum flux. Within this diagram, the Galactic sources form a sequence spanning a range of 3 orders of magnitude in these ratios, ranging from embedded compact H II regions to exposed Photo Dissociation Regions (PDRs) and the (diffuse) ISM. However, the variation in the 6.2 µm PAH feature-to-continuum ratio is relative small. Comparison -2 -of our extragalactic sample with our Galactic sources revealed an excellent resemblance of normal and starburst galaxies to exposed PDRs. While Seyfert-2's coincide with the starburst trend, Seyfert-1's are displaced by at least a factor 10 in 6.2 µm continuum flux, in accordance with general orientation dependent unification schemes for AGNs. ULIRGs show a diverse spectral appearance. Some show a typical AGN hot dust continuum. More, however, are either starburstlike or show signs of strong dust obscuration in the nucleus. One characteristic of the ULIRGs also seems to be the presence of more prominent FIR emission than either starburst galaxies or AGNs. We discuss the observed variation in the Galactic sample in view of the evolutionary state and the PAH/dust abundance and discuss the use of PAHs as quantitative tracers of star formation activity. Based on these investigations we find that PAHs may be better suited as a tracer of B stars, which dominate the Galactic stellar energy budget, than as a tracer of massive star formation (O stars).