Unexpectedly strong effect of caffeine on the vitality of western honeybees (Apis mellifera)

Strachecka, Aneta; Krauze, M; Olszewski, Krzysztof; Borsuk, Grzegorz; Paleolog, Jerzy; Merska, Malwina; Chobotow, Jacek; Bajda, M.; Grzywnowicz, Krzysztof

doi:10.1134/s0006297914110066

Cited by 46 publications

(84 citation statements)

References 42 publications

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“…7). Caffeine, which is a curcumin analogue (Anand et al, 2008), has similar effects (Strachecka et al, 2014a). Curcumin reduces blood glucose concentration in diabetic rats (Zhang et al, 2013).…”

Section: Curcumin Extend the Apian Resistance And Life Span Resultsmentioning

confidence: 99%

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Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

Strachecka

Olszewski

Paleolog

2015

Journal of Apicultural Science

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a b s t r a c t We examined the influence of curcumin-supplemented feeding on worker lifespan, Nosema resistance, key enzyme activities, metabolic compound concentrations and percentage of the global dna methylation. two worker groups (Apis mellifera) were set up: 1) control group; workers were fed ad libitum with sucrose syrup; 2) workers were fed with the syrup with the addition of curcumin. dead workers were removed every two days and the Nosema spp. infection levels were assessed. hemolymph was taken from living workers for biochemical analyses. the global dna methylation level was analysed using dna from worker heads and thoraces. the bees that consumed curcumin lived longer and were less infested with Nosema spp. the curcumin-treated workers had higher concentrations of proteins, non-enzymatic biomarkers (triglycerides, glucose, cholesterol, mg 2+ and Ca 2+ ), uric acid and creatinine, as well as elevated activities of antioxidant enzymes (sod, Gpx, Cat, Gst), neutral proteases, protease inhibitors, enzymatic biomarkers (ast, alt, alp). the concentrations of albumin and urea, and the activities of acidic and alkaline proteases were higher in the control group. Curcumin decreased global dna methylation levels especially in older bees in which the natural, age-related level increase was observed. most of the parameters increased over the apian youth and adulthood, and decreased in older bees. the decrease was markedly delayed in the bees fed with curcumin. Curcumin appeared to be an unexpectedly effective natural bio-stimulator, improving apian health and vitality. this multifactorial effect is caused by the activation of many biochemical processes involved in the formation of apian resistance.

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Section: Curcumin Extend the Apian Resistance And Life Span Resultsmentioning

confidence: 99%

“…They decrease, in turn, after supplementation (e.g. caffeine; Strachecka et al, 2014a). Therefore, we suppose that curcumin may influence not only biochemical but also epigenetic characteristics in honeybees, particularly global DNA methylation changes related with ageing (hypothesis 3).…”

mentioning

confidence: 99%

Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

Strachecka

Olszewski

Paleolog

2015

Journal of Apicultural Science

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“…Although various studies have been carried out on honeybees and other insects, there are only a limited number of studies on the catalase (CAT) activities of worker bees at different ages (Weirich et al, 2002;Strachecka et al, 2014aStrachecka et al, , 2014b. The aims of this study were to determine CAT activity, total RNA level, and total protein, which play a significant role in the honeybee life cycle in different age groups of worker honeybees.…”

Section: Introductionmentioning

confidence: 99%

Determination of some biochemical parameters of worker honeybees (Apis mellifera L.) belonging to different age groups

Akyol¹,

Selamoğlu²,

Gülhan³

2015

Turk J Zool

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The catalase activity, total protein, and total RNA levels of worker bees of different age groups (4, 9, 15, 20, and 24 days old) within the same honeybee colony were studied. The catalase activities of group I (4 days), group II (9 days old), group III (15 days old), group IV (20 days old), and group V (24 days old) were 4.10 ± 0.59, 4.32 ± 0.57, 4.32 ± 0.57, 4.41 ± 0.49, and 4.47 ± 0.48 kU/g, respectively. There were significant differences in total protein and total RNA levels among the different age groups of workers. The calculated total protein levels of group I, group II, group III,

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“…poprzez suplementację m.in. : koenzymem Q10 (41), kofeiną (40), fenyloacetyloglutaminianem sodu (PG) (30). Przy braku czynnika stresogennego lub patogennego metaloproteazy występują na kutikuli robotnic w postaci proenzymów.…”

Section: Metaloproteazy I Ich Inhibitory U Owadówunclassified

Mammalian and insect metalloproteases

Bajda¹,

Łoś²,

Schulz³

et al. 2016

Medycyna Weterynaryjna

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Metalloproteases (metalloproteinases, MMP) digest extracellular matrix proteins. They have zinc ions (Zn2+) in their active site. They are synthesized within cells as proenzymes, to be subsequently activated in the extracellular environment. MMP are active in a neutral or slightly alkaline pH in the presence of Ca2+ ions. Cells that synthesize metalloproteases also produce metalloprotease inhibitors. Until now, 4 of MMP inhibitors as well as 28 endometalloproteases have been discovered, out of which 22 occur in humans. On the other hand, these enzymes have not been well explored in insects, in which only 2 metalloproteases were identified (Ance and ECE). Their optimal activity ranges between pH 7.0 and 9.4. MMP inhibitors control the concentration of metalloproteases in physiological conditions. They fall within two types: specific tissue MMP inhibitors and non-specific plasma MMP inhibitors. Metalloproteases and their inhibitors play an important role in both physiological and pathological processes in the organism. Metalloproteases are cell growth promotors. They inhibit/induce apoptosis, stimulate the development of healthy cells and control the activity of neoplastic cells both in people and in insects. Their activity is increased in skin and periodontal diseases, in arthritis, arteriosclerosis or in the period following myocardial infarction. In insects the activity of MMPs is also increased by environmental pollution, by the use of antibiotics and varroacides. The insect MMPs participate in digestion, biosynthesis of peptide hormones and neurotransmitters, and melanisation. They also affect the development of the reproductive system and the development of larvae and pupae, as well as prevent pathogen invasions. Worthy of special attention is the insect cuticle defensive barrier associated with MMP. Activation of metalloproteases is dependent on the physiological state of the organism, as well as on environmental pressure. Analyzing activities of metalloproteases and their inhibitors enables better monitoring of the pathological conditions in both insects and mammals.

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Unexpectedly strong effect of caffeine on the vitality of western honeybees (Apis mellifera)

Cited by 46 publications

References 42 publications

Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

Curcumin Stimulates Biochemical Mechanisms of Apis Mellifera Resistance and Extends the Apian Life-Span

Determination of some biochemical parameters of worker honeybees (Apis mellifera L.) belonging to different age groups

Mammalian and insect metalloproteases

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