Undetectable apolipoprotein<i>A-I</i>gene expression suggests an unusual mechanism of dietary lipid mobilisation in the intestine of<i>Cyprinus carpio</i>

Concha, Miguel; López, Rodríguez; Villanueva, Julieta; Báez, Nadya; Amthauer, Rodolfo

doi:10.1242/jeb.01512

Cited by 9 publications

(8 citation statements)

References 32 publications

(36 reference statements)

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“…In lipid transport, we have identified several spots as apolipoprotein A-I (ApoAI), as being down regulated in T1 gilthead sea bream plasma. This protein is produced in the liver and intestine (Llewellyn et al 1998), and it is involved in lipid absorption in the intestine (Panzenbock et al 2000;Concha et al 2005;Geay et al 2011) and also in the transport of cholesterol to the liver by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (Ibarz et al 2010;Geay et al 2011). In our study, this protein could be related to lipid metabolism, as a representation of lipid recruitment for metabolic processes (Geay et al 2011).…”

Section: Discussionmentioning

confidence: 76%

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Physiological responses of reared sea bream (Sparus aurata Linnaeus, 1758) to an Amyloodinium ocellatum outbreak

Moreira

Schrama

Soares

et al. 2017

Journal of Fish Diseases

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Amyloodiniosis represents a major bottleneck for semi-intensive aquaculture production in Southern Europe, causing extremely high mortalities. Amyloodinium ocellatum is a parasitic dinoflagellate that can infest almost all fish, crustacean and bivalves that live within its ecological range. Fish mortalities are usually attributed to anoxia, associated with serious gill hyperplasia, inflammation, haemorrhage and necrosis in heavy infestations; or with osmoregulatory impairment and secondary microbial infections due to severe epithelial damage in mild infestation. However, physiological information about the host responses to A. ocellatum infestation is scarce. In this work, we analysed the proteome of gilthead sea bream (Sparus aurata) plasma and relate it with haematological and immunological indicators, in order to enlighten the different physiological responses when exposed to an A. ocellatum outbreak. Using 2D-DIGE, immunological and haematological analysis and in response to the A. ocellatum contamination we have identified several proteins associated with acute-phase response, inflammation, lipid transport, homoeostasis, and osmoregulation, wound healing, neoplasia and iron transport. Overall, this preliminary study revealed that amyloodiniosis affects some fish functional pathways as revealed by the changes in the plasma proteome of S. aurata, and that the innate immunological system is not activated in the presence of the parasite.

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Section: Discussionmentioning

confidence: 76%

“…; Concha et al . ; Geay et al . ) and also in the transport of cholesterol to the liver by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (Ibarz et al .…”

Section: Discussionmentioning

confidence: 99%

Physiological responses of reared sea bream (Sparus aurata Linnaeus, 1758) to an Amyloodinium ocellatum outbreak

Moreira

Schrama

Soares

et al. 2017

Journal of Fish Diseases

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“…A similar phenomenon was also detected for Takifugu rubripes ApoA-I (21% identity) [ 12 ], Danio rerio ApoA-I (25.6% identity) [ 33 ], Oncorhynchus mykiss ApoA-I.1 and ApoA-I.2 (28% identity) [ 28 ] and Cyprinus carpio ApoA-I (27% identity) [ 18 ]. Expression patterns of apoA-I have been reported in several fish species [ 13 , 14 , 15 , 16 , 17 , 25 , 33 ], but studies on temporal and spatial expression patterns of apoA-I were not performed in common carp. The expression pattern of apoA-Ib in embryonic development of common carp ( Figure 3 A) was similar to that of zebrafish [ 33 ] and turbot [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Kondo et al [ 12 ] pointed out that a low homology existed between fish apolipoproteins and their counterparts in mammals. The tissue expression analyses of apoA-I in some fish species showed that the liver was always observed with the highest expression, but more variable expression levels were detected in the intestine, gills and epidermis [ 13 , 14 , 15 , 16 , 17 , 18 ]. The antibacterial properties of ApoA-I have also been detected in several fish species [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Growth Association of Two Apolipoprotein A-Ib Genes in Common Carp (Cyprinus carpio)

Wang

Tong

2016

IJMS

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Apolipoprotein A-I (ApoA-I) is functionally involved in the transportation and metabolism of lipids in vertebrates. In this study, two isoforms of apoA-Ib in common carp (Cyprinus carpio L.) were characterized. Sequence comparison and phylogenetic analysis showed that C. carpio ApoA-Ib is relatively conserved within cyprinid fishes. During embryonic development, C. carpio apoA-Ib was first expressed at the stage of multi-cells, and the highest mRNA level was observed at the stage of optic vesicle. A ubiquitous expression pattern was detected in various tissues with extreme predominance in the liver. Significantly different expression levels were observed between light and heavy body weight groups and also in the compensatory growth test. Seventeen and eight single-nucleotide polymorphisms (SNPs) were identified in matured mRNA of the C. carpio apoA-Ib.1 and apoA-Ib.2, respectively. Two of these SNPs (apoA-Ib.2-g.183A>T and apoA-Ib.2-g.1753C>T) were significantly associated with body weight and body length in two populations of common carp. These results indicate that apoA-Ib may play an important role in the modulation of growth and development in common carp.

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“…Although the mechanism for this antimicrobial activity remains unclear, it is conceivable that the detergent capacity demonstrated for ApoA-I from different species including fish, evidenced by its ability to solubilise dimyristoylphosphatydylcholine multilamellar vesicles and remove different kind of lipids from cell membranes could be relevant for this activity [30,31]. The antimicrobial activity observed should be physiologically relevant since we determined an average plasma concentration of 366 mM (w10 mg mL À1 ) for trout ApoA-I, both in healthy and sick fish.…”

Section: Discussionmentioning

confidence: 99%

Apolipoprotein A-I, an antimicrobial protein in Oncorhynchus mykiss: Evaluation of its expression in primary defence barriers and plasma levels in sick and healthy fish

Villarroel

Bastías

Casado

et al. 2007

Fish & Shellfish Immunology

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107

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Undetectable apolipoproteinA-Igene expression suggests an unusual mechanism of dietary lipid mobilisation in the intestine ofCyprinus carpio

Cited by 9 publications

References 32 publications

Physiological responses of reared sea bream (Sparus aurata Linnaeus, 1758) to an Amyloodinium ocellatum outbreak

Physiological responses of reared sea bream (Sparus aurata Linnaeus, 1758) to an Amyloodinium ocellatum outbreak

Molecular Characterization and Growth Association of Two Apolipoprotein A-Ib Genes in Common Carp (Cyprinus carpio)

Apolipoprotein A-I, an antimicrobial protein in Oncorhynchus mykiss: Evaluation of its expression in primary defence barriers and plasma levels in sick and healthy fish

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