Understanding functional implication of β-casein gene variants in four cattle breeds characterized using AmpliSeq approach

Patel, Shiven B.; Shah, Tejas; Sabara, Pritesh; Bhatia, Dhruv; Panchal, Ketankumar; Italiya, Jignesh; Koringa, Prakash G.; Rank, D. N.

doi:10.1007/s13205-020-02410-2

Cited by 15 publications

(12 citation statements)

References 14 publications

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“…The remainder of the protein fraction is composed of other caseins (α-s1, α-s2 and κ) and serum proteins (α-lactalbumin and β-lactoglobulin) (BROOKE-TAYLOR et al, 2017;DANILOSKI et al, 2021). Twenty-two variants (A1, A2, A3, A4, B, C, D, E, F, H1, H2, I, G) were found in the β-casein (β-CN) (FARRELL et al, 2004;FERRETTI, LEONE & SGARAMELLA, 1990;NGUYEN et al, 2018;PATEL et al, 2020). The position and composition of the chain profile of 209 amino acids classifies the variants of β-casein (GORKHALI et al, 2021).…”

Section: What Is A2 Milk?mentioning

confidence: 99%

“…Several studies have been published and the subject has gained increasing relevance in the scientific community (BARBOSA et al, 2019;BROOKE-TAYLOR et al, 2017;DANILOSKI et al, 2021;NGUYEN et al, 2018;PATEL et al, 2020;THIRU-VENGADAM et al, 2021). Previous and recent studies have pointed to the fact that the consumption of conventional A1/A2 milk has negative effects on the health of some individuals.…”

Section: What Is A2 Milk?mentioning

confidence: 99%

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2022

DesDeb

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Desenvolvimento em Debate ¿Hacia dónde va la integración sudamericana? Claves para pensar el futuro del MERCOSUR... v.10, n.2, maio-ago. 2022, p.11-27 11 ResumenLos 30 años del MERCOSUR constituyen un hito simbólico que abre la posibilidad de repensar el presente y el futuro de la integración regional en el Cono Sur a la luz de las experiencias y recorridos atravesados. En este artículo nos proponemos indagar en ocho puntos que constituyen la columna vertebral del proceso.Son elementos que se vienen prefigurando desde el inicio mismo de los acercamientos que dieron origen al MERCOSUR, pero que hoy asumen una relevancia diferente. Además, la dinámica que adquieran en los próximos años le darán una forma particular al futuro de una región tensionada por proyectos en pugna. Anclados en la concepción de la integración regional como política pública y en la noción de las políticas públicas regionales como estructurante para comprender las iniciativas en el marco del MERCOSUR, exploramos las disyuntivas actuales de cara a la construcción del proceso a mediano y largo plazo.

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Section: What Is A2 Milk?mentioning

confidence: 99%

Section: What Is A2 Milk?mentioning

confidence: 99%

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2022

DesDeb

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“…The heterogeneity of milk proteins includes genetic polymorphisms that have received great interest in the last decade among the dairy industries and also the consumer because of their possible association with milk composition, processing attributes, and human health concerns (The BCM‐7 release). Nowadate, a total of fourteen different genetic variants of beta‐casein (β‐Cn) have been identified in several investigations; A (A 1 , A 2 , A 3 , and A 4 ), B, C, D, E, F, G, H (H 1 and H 2 ), I and J (Patel et al ., 2020). The peptic digestion of the A 1 β‐Cn chain yields β‐casomorphin‐7 (BCM‐7), which is significantly correlated with many health issues like autism at an early age, cardiovascular risks, diabetes, ischaemic heart disease, etc.…”

Section: Introductionmentioning

confidence: 99%

Identification of β‐casein phenotypes (A¹/A²) in the milk of the Indian Jersey crossbreed bovine using the high‐resolution accurate mass spectrometer

Singh

Kumar

Chandra

et al. 2023

Int J of Food Sci Tech

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SummaryFoodomics is an emerging probing method of phenotype investigation of the different milk proteins and their subtypes. The polymorphic nature of the β‐casein (β‐Cn) protein has shown fourteen different protein variants to date in bovines. The analysis of the β‐Cn genetic polymorphism from the milk of the crossbred dairy animals is crucial for the quality assurance of the consumers from the various health concerns, especially those linked with the A1 phenotype which yields β‐casomorphin‐7 on in‐vivo digestion. Jersey‐crossed Indian cattle have been widely utilized in dairy because of their better milk production and survival performance trait. In this investigation, an SDS‐PAGE coupled with a high‐resolution accurate mass spectrometry‐based proteomics approach has been applied to identify the presence of specific phenotype of the β‐Cn protein in the milk of the 24 Indian crossbred (Jersey crossed) animals. Amino acid sequential analysis has been done using different search modules, as MS Amanda and Sequest HT showed 17 cows are producing A2 β‐Cn (Pro~67) while only seven animals yielded the A1 variant (His~67). The maximum number of Indian Jersey‐crossed animals are lactating milk having A2 β‐Cn. The A2 milk from the crossbred animals is free from the negative impact on health caused by β‐casomorphin‐7 (BCM‐7) released during digestion of the A1 phenotype. Among the molecular biology techniques, top‐down proteomics has been an intriguing technique for the identification of protein genetic polymorphic products.

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“…The copyright holder for this preprint this version posted August 27, 2022. ; https://doi.org/10.1101/2022.08.27.505546 doi: bioRxiv preprint temperate climatic conditions (Mwai et al, 2015;Cooke et al, 2020). Furthermore, the Indicine cattle breeds produce milk rich in A2 casein variant, which is better for health (Mishra et al, 2009), whereas the B. taurus breeds are generally carriers of A1 casein variant (Kamiński et al, 2007;Patel et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing and de novo and reference-based genome assemblies of Bos indicus breeds

Chakraborty

Bisht

Saxena

et al. 2022

Preprint

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Bos indicus is a domestic cattle species with many indigenous breeds found in India and is important for dairy, draught work, and other household activities. Distinct phenotypic differences are observed among the breeds of this species; however, their whole genome sequences were not available. Therefore, in this study, we performed the whole genome sequencing using Illumina short-read technology to construct draft genome assemblies of four B. indicus breeds; Ongole, Kasargod Dwarf, Kasargod Kapila, and Vechur, of which Vechur is known as the smallest cow of the world. We also report the first de novo genome assemblies of these native B. indicus breeds. Further, we constructed the 18S rRNA marker gene sequences of these B. indicus breeds, which were not yet known. Genomic analysis helped to identify the distinct bovine phenotypic characteristics-related and other biological process-related genes in this species compared to B. taurus, and to gain comparative genomic insights between the dwarf and non-dwarf breeds of this species.

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Understanding functional implication of β-casein gene variants in four cattle breeds characterized using AmpliSeq approach

Cited by 15 publications

References 14 publications

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Identification of β‐casein phenotypes (A¹/A²) in the milk of the Indian Jersey crossbreed bovine using the high‐resolution accurate mass spectrometer

Genome sequencing and de novo and reference-based genome assemblies of Bos indicus breeds

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Understanding functional implication of β-casein gene variants in four cattle breeds characterized using AmpliSeq approach

Cited by 15 publications

References 14 publications

Untitled

Untitled

Identification of β‐casein phenotypes (A1/A2) in the milk of the Indian Jersey crossbreed bovine using the high‐resolution accurate mass spectrometer

Genome sequencing and de novo and reference-based genome assemblies of Bos indicus breeds

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Product

Resources

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Identification of β‐casein phenotypes (A¹/A²) in the milk of the Indian Jersey crossbreed bovine using the high‐resolution accurate mass spectrometer