Unbiased stereological analysis of the fate of oligodendrocyte progenitor cells in the adult mouse brain and effect of reference memory training

Boulanger, Jenna J.; Messier, Claude

doi:10.1016/j.bbr.2017.04.027

Cited by 7 publications

(9 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For all estimates, coefficients of error ≤ 0.10 were accepted; as a result, 2 of the 5 data points for each data set were removed resulting in a final group size of 3. While low, the group sizes are sufficient based on previous stereology publications (Boulanger and Messier, 2017; West, 2013). The data was divided into left and right subdivisions of the lateral amygdala (lateral dorsolateral, lateral ventrolateral, and lateral ventromedial) and basolateral amygdala (basolateral anterior, basolateral posterior and basolateral ventral) for separate analyses.…”

Section: Methodsmentioning

confidence: 99%

Hemispheric differences in the number of parvalbumin-positive neurons in subdivisions of the rat basolateral amygdala complex

et al. 2018

View full text Add to dashboard Cite

The amygdala is a bilateral temporal lobe brain region which plays an important role in emotional processing. Past studies on the amygdala have shown hemispheric differences in amygdalar processes and responses associated with specific pain and fear behaviors. Despite the functional differences in the amygdala, few studies have been performed to characterize whether anatomical differences exist between the left and right amygdala. Parvalbumin (PV) is a phenotypic marker for an inhibitory interneuronal population in cortical brain structures such as the basolateral amygdala complex (BLC). This study examined the number of PV-positive neurons in the left and right BLC of adult, male Long-Evans rats using unbiased stereology. Coronal sections through the rostral-caudal extent of the BLC were immunohistochemically-stained for PV and the optical fractionator method was used to obtain an unbiased estimate of the number of PV-positive neurons in subdivisions through the BLC. The lateral and basolateral amygdala divisions of the BLC were analyzed, were subdivided into the dorsolateral, ventrolateral and ventromedial and the posterior, anterior and ventral subdivisions, respectively. The results indicate that there are significantly more PV-positive neurons in the left basolateral amygdala compared to the right, with a significant difference specifically in the posterior subdivision. This difference in PV neuronal number could help explain the distinct hemispheric roles of the BLC in the behavioral processing following exposure to painful and fearful stimuli.

show abstract

Section: Methodsmentioning

confidence: 99%

Hemispheric differences in the number of parvalbumin-positive neurons in subdivisions of the rat basolateral amygdala complex

et al. 2018

View full text Add to dashboard Cite

show abstract

“…They differentiate to replenish myelin within the CNS [ 75 ]. Evidence indicates OPCs of developing and adult brain also undergo neuronal differentiation [ 76 ]. However, the presence of a circadian clock in oligodendrocytes and their progenitors has yet to be demonstrated.…”

Section: Neural Stem Cells and Circadian Rhythmsmentioning

confidence: 99%

“…Accordingly, Tang et al (2021) recently reported that exercise by running can reverse depression-like behavior in mice exposed to unpredictable chronic stress by increasing oligodendrocyte differentiation and myelination in the CA1 subfield of the hippocampus [ 78 ]. Evidence indicates OPCs also undergo neuronal differentiation [ 76 ]. Adult neurogenesis from OPCs has been reported in vitro and in the cerebral cortex in vivo [ 79 , 80 ].…”

Section: Neural Stem Cells and Circadian Rhythmsmentioning

confidence: 99%

The Relevance of Circadian Clocks to Stem Cell Differentiation and Cancer Progression

Malik

Nalluri

et al. 2022

NeuroSci

View full text Add to dashboard Cite

The molecular mechanism of circadian clocks depends on transcription-translation feedback loops (TTFLs) that have known effects on key cellular processes. However, the distinct role of circadian TTFLs in mammalian stem cells and other less differentiated cells remains poorly understood. Neural stem cells (NSCs) of the brain generate neurons and glia postnatally but also may become cancer stem cells (CSCs), particularly in astrocytomas. Evidence indicates clock TTFL impairment is needed for tumor growth and progression; although, this issue has been examined primarily in more differentiated cancer cells rather than CSCs. Similarly, few studies have examined circadian rhythms in NSCs. After decades of research, it is now well recognized that tumors consist of CSCs and a range of other cancer cells along with noncancerous stromal cells. The circadian properties of these many contributors to tumor properties and treatment outcome are being widely explored. New molecular tools and ones in development will likely enable greater discrimination of important circadian and non-circadian cells within malignancies at multiple stages of cancer progression and following therapy. Here, we focus on adult NSCs and glioma CSCs to address how cells at different stages of differentiation may harbor unique states of the molecular circadian clock influencing differentiation and cell fate.

show abstract

“…A single homeodomain transcription factor Dlx2‐transfects NG2‐glia differentiated into GABAergic inhibitory neurons 19 . Adult NG2‐glia also differentiate into neurons in the cortical gray matter 21 . The anti‐epileptic epigenetic regulator valproic acid accelerates the differentiation of neurons from NG2‐glia with neurogenic means in vitro 17 …”

Section: Introductionmentioning

confidence: 99%

“…19 Adult NG2-glia also differentiate into neurons in the cortical gray matter. 21 The anti-epileptic epigenetic regulator valproic…”

mentioning

confidence: 99%

NG2‐glia crosstalk with microglia in health and disease

Zuo

Zhou

et al. 2022

CNS Neurosci Ther

View full text Add to dashboard Cite

Glia are non-neuronal cells, also called neuroglia. 1 Glia exist within the mammalian nervous system to provide several essential functions for neurons, such as maintaining homeostasis, support, protection, and generating myelin. 2,3 Traditionally, astrocytes, microglia, and oligodendrocytes are the three major classes of glia. In the last two decades, neuron glia antigen-2 (NG2) glial cell has been considered as the fourth type of glial cell in the central nervous system (CNS). 4 NG2-glia are also called oligodendrocyte precursor cells 5 and express NG2. [6][7][8] NG2 is a chondroitin sulphate proteoglycan 4 (CSPG4), which is also expressed on infiltrating macrophages and activated microglia in various CNS insults. 9-11 NG2, together with platelet-derived growth factor receptor alpha (PDGFRα), is a marker of NG2-glia. 12,13 NG2-glia account for 5%-10% of all cells in the adult CNS. 14 NG2-glia are extremely heterogeneous with different characteristics and functions. 15,16 NG2-glia are widely scattered in the CNS in late development and throughout adulthood. NG2-glia may differentiate into neurons or astrocytes under diverse conditions, such as at embryonic stages, and under forced expression of neurogenic factors. [17][18][19] Early postnatal NG2-glia can become multipotent neurospheres and quickly differentiate into neurons, astrocytes, and oligodendrocytes in vitro. 18 More than one-third of the protoplasmic astrocytes are differentiated from NG2-glia in ventral forebrain when NG2-Cre is prenatally triggered in NG2-glia before embryonic day 17.5. 20 A single homeodomain transcription factor Dlx2-transfects NG2-glia differentiated into GABAergic inhibitory neurons. 19 Adult NG2-glia also differentiate into neurons in the cortical gray matter. 21 The anti-epileptic epigenetic regulator valproic

show abstract

Unbiased stereological analysis of the fate of oligodendrocyte progenitor cells in the adult mouse brain and effect of reference memory training

Cited by 7 publications

References 83 publications

Hemispheric differences in the number of parvalbumin-positive neurons in subdivisions of the rat basolateral amygdala complex

Hemispheric differences in the number of parvalbumin-positive neurons in subdivisions of the rat basolateral amygdala complex

The Relevance of Circadian Clocks to Stem Cell Differentiation and Cancer Progression

NG2‐glia crosstalk with microglia in health and disease

Contact Info

Product

Resources

About