Ultrastructural study of a case of extramammary Paget's disease of the vulva

Ultrastructural Examination of a Case of Pagetoid Bowen Disease Exhibiting Immunohistochemical Features in Common With Extramammary Paget Disease

“…[11][12][13] However, EMPD typically shows large nuclei, several nucleoli, abundant Golgi apparatus and free ribosomes, mitochondrial polymorphism with giant mitochondria, intracytoplasmic and intercellular glycogen particles, microvesicular bodies, myelin figures, pale filament bundles that have no connection with the plasma membrane, small desmosomes, and microvilli. [14][15][16] Our case showed some features of BD, including irregular condensed chromatin pattern, large-sized nucleoli, few melanosomes, wide intercellular spaces, and condensation of tonofilaments resulting in dyskeratosis and, at the same time, also showed some features of EMPD, including central round nuclei, abundant clear cytoplasm, well-developed Golgi apparatus, numerous free ribosomes, small desmosomes with short condensed tonofilaments and intracytoplasmic glycogen granules located adjacent to the cytoplasmic membrane. Interestingly, similar ultrastructural features have been previously observed in 2 cases of EMPD with bowenoid components by Yamamura et al 17 The presence of features of both BD and EMPD could be explained if the tumor originates from multipotent stem cells capable of further differentiation into squamous and secretory lines.…”

Distinguishing tumour cells of mammary from extramammary Paget's disease using antibodies to two different glycoproteins from human milk-fat-globule membrane

“…Several investigators using both light and electronmicroscopical studies have proposed a variety of cell-types as the progenitors of Paget's cells, especially the extramammary variety (Ashikari et al, 1970;Demopoulos, 1970;Ferenczy & Richart, 1972;Fetherston & Friedrich, 1972;Lee et al, 1977;Mazonjian et al, 1984;Medenica and Sahihi, 1972;Orr and Parish, 1962;Roth et al, 1977;Sagebiel, 1969;Paone & Beker, 1981). The controversy over the histogenesis of Paget's cells has diverged into two main lines of opinion, viz.…”

Histochemical observation of lectin-binding patterns in Paget's disease.

“…Belcher (2) stated enzyme histochemically that Paget cells in the breast contain higher activities of phosphorylase, leucine aminopeptidase, and that positive activities of succinate dehydrogenase, cytochrome oxidase, acid phosphatase and esterase showed an apocrine enzymatic pattern. Electron microscopic features have noted that Paget cells contain lysosome-like bodies and autophagic vacuoles (2,8,22,25). Such histochemical and ultrastructural evidence may suggest the presence of certain lysosomal bodies or related bodies in the vacuolated materials in Paget cells.…”