Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells

Taskaeva, Iuliia; Bgatova, Nataliya

doi:10.31557/apjcb.2018.3.3.83-87

Cited by 6 publications

(5 citation statements)

References 23 publications

(25 reference statements)

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“…Similar to the vast majority of cancer types, HCC may be caused by environmental and genetic factors (41)(42)(43)(44). Despite improvements in surgical methods and the therapeutics available, the survival rate of patients with HCC remains unsatisfactory (45).…”

Section: Discussionmentioning

confidence: 99%

snoRNA23 enhances the progression of hepatocellular carcinoma via regulation of the Wnt/β-catenin pathway

Qiao

Zhang

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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Small nucleolar non-coding RNA(snoRA)23 is upregulated in human pancreatic ductal adenocarcinoma. However, to the best of our knowledge, the role of snoRA23 in hepatocellular carcinoma progression has not been determined. MTT and colony formation assays were used to assess the cell viability and proliferation of HCC cells with snoRA23 knocked down, respectively, and a lymphatic vessel formation assay was used to determine tube formation ability of Human dermal lymphatic endothelial cells treated with conditioned media from HCC cell cultures. The results showed that snoRA23 knockdown attenuated cell viability, colony formation,and lymphatic vessel formation in HCC cells. snoRA23 was correlated with the prolonged overall survival of patients with HCC. Additionally, snoRA23 knockdown downregulated the Wnt/β-catenin signaling pathway by decreasing Wnt3a expression and β-catenin levels.β-methylacyl-CoA racemase (AMACR) levels were notably decreased by snoRA23 depletion. Finally, it was confirmed that AMACR overexpression partially rescued snoRA23-modulated HCC tumorigenesis. The results of the present study provide further insight into the role of non-coding RNAs in the development and progression of HCC.

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Section: Discussionmentioning

confidence: 99%

snoRNA23 enhances the progression of hepatocellular carcinoma via regulation of the Wnt/β-catenin pathway

Qiao

Zhang

et al. 2021

Cell Mol Biol (Noisy-le-grand)

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“…Additionally, lithium carbonate promotes autophagic vacuole formation in hepatocellular carcinoma (HCC) cells in vivo, suggesting that lithium-mediated autophagy might be a novel approach for treating hepatocellular carcinoma [ 133 ]. Interestingly, in HCC, combinatory treatment of lithium carbonate and rapamycin increases autolysosome formation and the expression of autophagy markers such as LC3β and LAMP1 [ 134 ].…”

Section: Effect Of Lithium On Certain Cancer Hallmarksmentioning

confidence: 99%

“…Therefore, LiCl promotes apoptosis through the Akt signaling pathway and cell cycle arrest by increasing phosphorylation of GSK3β [ 137 ]. In addition, lithium carbonate increases the number and volume of autophagic vacuoles and increases levels of LC3β, a biomarker of autophagy death [ 133 ]. Additionally, Li 2 CO 3 induces apoptosis and arrests the cell cycle in the G2/M phase in hepatocellular carcinoma-29 cells [ 33 ].…”

Section: Effect Of Lithium On Certain Cancer Hallmarksmentioning

confidence: 99%

Lithium: A Promising Anticancer Agent

Villegas-Vázquez

Quintas-Granados

Cortés

et al. 2023

Life

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Lithium is a therapeutic cation used to treat bipolar disorders but also has some important features as an anti-cancer agent. In this review, we provide a general overview of lithium, from its transport into cells, to its innovative administration forms, and based on genomic, transcriptomic, and proteomic data. Lithium formulations such as lithium acetoacetate (LiAcAc), lithium chloride (LiCl), lithium citrate (Li3C6H5O7), and lithium carbonate (Li2CO3) induce apoptosis, autophagy, and inhibition of tumor growth and also participate in the regulation of tumor proliferation, tumor invasion, and metastasis and cell cycle arrest. Moreover, lithium is synergistic with standard cancer therapies, enhancing their anti-tumor effects. In addition, lithium has a neuroprotective role in cancer patients, by improving their quality of life. Interestingly, nano-sized lithium enhances its anti-tumor activities and protects vital organs from the damage caused by lipid peroxidation during tumor development. However, these potential therapeutic activities of lithium depend on various factors, such as the nature and aggressiveness of the tumor, the type of lithium salt, and its form of administration and dosage. Since lithium has been used to treat bipolar disorder, the current study provides an overview of its role in medicine and how this has changed. This review also highlights the importance of this repurposed drug, which appears to have therapeutic cancer potential, and underlines its molecular mechanisms.

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“…The prevalence of hepatocellular carcinoma is increasing in the western countries. However, this disease has been a serious health problem in Asia and Africa for a long time (Lin et al, 2017;Ayuso et al, 2018;Taskaeva and Bgatova, 2018).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Apoptotic Gene Expression in Hepatoma Cell Line (HepG2) Following Nisin Treatment

Zainodini

Hajizadeh

Mirzaei

2021

Asian Pac J Cancer Prev

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Objective: The present study aims to examine the effects of nisin on the survival and apoptosis of the hepatoma cell line HepG2 and to investigate possible apoptosis pathways activated by nisin. Materials and Methods: For this purpose, viability and apoptosis of the cells were accomplished by the nisin treatment using the MTT assay and Annexin-V-fluorescein/propidium iodide (PI) double staining, respectively. Additionally, the human apoptosis PCR array was performed to determine pathways or genes activated by nisin during possible apoptosis. Results: The results of the present study showed that nisin was able to decrease cell viability (IC 50 ~ 40 µg/ml) in a dose-dependent manner and could induce apoptosis in HepG2 cells. PCR data indicated a considerable increase in the expression of genes, such as caspase and BCL2 families, involved in the induction of apoptosis. Conclusions: The data from this study showed that overexpression of genes involved in the intrinsic pathway of apoptosis, especially caspase-9 and BID, increased apoptosis in HepG2 cells treated by nisin, compared to the control group.

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Ultrastructural and Immunofluorescent Analysis of Lithium Effects on Autophagy in Hepatocellular Carcinoma Cells

Cited by 6 publications

References 23 publications

snoRNA23 enhances the progression of hepatocellular carcinoma via regulation of the Wnt/β-catenin pathway

snoRNA23 enhances the progression of hepatocellular carcinoma via regulation of the Wnt/β-catenin pathway

Lithium: A Promising Anticancer Agent

Evaluation of Apoptotic Gene Expression in Hepatoma Cell Line (HepG2) Following Nisin Treatment

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