Ultrastructural analysis of the spermatogenesis in the guinea pig (Cavia porcellus)

Abstract

al for both, the establishment of appropriate management systems, and for the use of new species as animal models. In this study, we used light and electron microscopy to characterize the sexual development stages of the guinea pig (Cavia porcellus) in specimens of 30, 45 and 90 days of age. We observed the differentiation of spermatocytes only through transmission electron microscopy in the leptotene, zygotene and pachytene phases of meiosis, in 30-day-old animals. During puberty, there was differentiation of the germinative epithelium and formation of the acrosome. Spermatozoa, however, were not detected. Thus, we could infer that puberty happens after 45 days of age. Sexual maturity was evident in 90-day-old specimens. Our results showed that changes in the testicular germinative epithelium during the postnatal sexual development in guinea pig led to morphological changes, including the ones related to the development of Leydig and Sertoli cells, which are directly related to puberty. In this work, we provide new morphological subsidies for a better understanding of reproductive parameters of this species, enabling its use as an animal model in the field of the reproductive biology.

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

“…In this regard in particular, the adult male guinea pigs were selected at ± 48-months of age, when their testosterone level was low. Additionally, in guinea pigs the structure of the testicles [25], epididymis [26] and spermatogenesis [27] have been studied in detail. The abovementioned data served as a justi cation for the use of guinea pigs in this experimental study.…”

The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

“…In this regard especially, the adult male guinea pigs were selected ± 48-months of age, which their testosterone level is low. Additionally in guinea pigs have been studied in detail the structure of testicles [29], epididymis [30] and spermatogenesis [31]. The above-mentioned data served as a justi cation for the use of guinea pigs in this experimental study.…”

Impact of groundwater samples and leachates from Gbagede dumpsite, Amoyo, Kwara State, Nigeria, on testes and prostate of male Wistar rats: A biochemical and histological study

“…The animals were exposed to the distilled water, groundwater samples (GW1 and GW2) and leachates (2.5%, 5.0%, 7.5% and 10.0%) ad libitum for 60 days. The 60‐day experimental period adopted in this study was informed by the 53 days of the spermatogenic process (1 complete cycle of spermatogenesis) and maximum of 7 days of final transit through the epididymis (Simões et al., 2016). The intake of GW1, GW2 and the leachates and the amount of feed consumed by the male rats were monitored on days 1, 14, 28 and 56 as described by Adams and Yakubu (2020).…”