Ultrasonic Bioreactor as a Platform for Studying Cellular Response

Subramanian, Anuradha; Turner, Joseph A.; Budhiraja, Gaurav; Thakurta, Sanjukta Guha; Whitney, Nicholas P.; Nudurupati, Sai Siddhartha

doi:10.1089/ten.tec.2012.0199

Cited by 27 publications

(35 citation statements)

References 40 publications

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“…An investigation into the macroscopic effects of US in the bioreactor used in the current research (Subramanian et al 2013) showed that the employed US stimulation did not result in any thermal or cavitational effects. Reactive oxygen species, a possible byproduct of sonication, was not detected (Subramanian et al 2013).…”

Section: Methodsmentioning

confidence: 72%

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Mechanotransduction of Ultrasound is Frequency Dependent Below the Cavitation Threshold

Louw

Budhiraja

Viljoen

et al. 2013

Ultrasound in Medicine & Biology

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This study provides evidence that low-intensity ultrasound directly affects nuclear processes, and the magnitude of the effect varies with frequency. In particular, we show that the transcriptional induction of first load-inducible genes, which is independent of new protein synthesis, is frequency dependent. Bovine chondrocytes were exposed to low-intensity below the cavitational threshold) ultrasound at 2,5 and 8 MHz. Ultrasound elevated the expression of early response genes c-Fos, c-Jun and c-Myc, maximized at 5 MHz. The phosphorylated ERK inhibitor PD98059 abrogated any increase in c-series gene expression, suggesting that signaling occurs via the MAPPK/ERK pathway. However, phosphorylated ERK levels did not change with ultrasound frequency, indicating that processes downstream of ERK phosphorylation (such as nuclear transport and chromatin reorganization) respond to ultrasound with frequency dependence. A quantitative, biphasic mathematical model based on Biot theory predicted that cytoplasmic and nuclear stress is maximized at 5.2 ± 0.8 MHz for a chondrocyte, confirming experimental measurements.

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Section: Methodsmentioning

confidence: 72%

“…Reactive oxygen species, a possible byproduct of sonication, was not detected (Subramanian et al 2013). This provides sufficient reason to believe that any bioeffect reported in this study is not caused by cavitation, but is related to the subtle effects of low-intensity US.…”

Section: Methodsmentioning

confidence: 99%

Mechanotransduction of Ultrasound is Frequency Dependent Below the Cavitation Threshold

Louw

Budhiraja

Viljoen

et al. 2013

Ultrasound in Medicine & Biology

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“…The ultrasonic field in a lab-scale bioreactor was simulated to illustrate the real-life application of the TM/ASA [21]. Fig.…”

Section: Ultrasonic Field In the Bioreactormentioning

confidence: 99%

“…The stationary wave field generated by the continuous application of ultrasound in a bioreactor is investigated as a model problem to test the applicability of the TM/ASA [21] (see Fig. 1).…”

Section: Introductionmentioning

confidence: 99%

Simulation of acoustic fields in fluid-, solid- and porous layers by the combined transfer matrix/angular spectrum approach with applications in bioacoustics

et al. 2015

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“…Further, for certain cell types there is evidence that the microenvironment provided by ultrasonic excitation can modify and enhance the production of the cells' extra cellular matrix [85,86]. A detailed study of the response of chondrocytes to culture within polymer scaffolds under ultrasonic excitation [87] observed positive changes in viability, proliferation, and gene expression, and modified expression of a large number of proteins, in comparison with control samples in the absence of ultrasonic excitation. One relevant application of ultrasound is for enhancing the seeding of tissue engineering scaffolds.…”

Section: Assembly and Tissue Engineeringmentioning

confidence: 99%

Ultrasound assisted particle and cell manipulation on-chip

Mulvana

Cochran

Hill

2013

Advanced Drug Delivery Reviews

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Ultrasonic fields are able to exert forces on cells and other micron-scale particles, including microbubbles. The technology is compatible with existing lab-on-chip techniques and is complementary to many alternative manipulation approaches due to its ability to handle many cells simultaneously over extended length scales. This paper provides an overview of the physical principles underlying ultrasonic manipulation, discusses the biological effects relevant to its use with cells, and describes emerging applications that are of interest in the field of drug development and delivery on-chip.

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Ultrasonic Bioreactor as a Platform for Studying Cellular Response

Cited by 27 publications

References 40 publications

Mechanotransduction of Ultrasound is Frequency Dependent Below the Cavitation Threshold

Mechanotransduction of Ultrasound is Frequency Dependent Below the Cavitation Threshold

Simulation of acoustic fields in fluid-, solid- and porous layers by the combined transfer matrix/angular spectrum approach with applications in bioacoustics

Ultrasound assisted particle and cell manipulation on-chip

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