Ultrasmall Superparamagnetic Iron Oxide Nanoparticles for Magnetic Resonance Imaging Contrast Agent

Zhao, Xueling; Zhao, Hongli; Chen, Zongyan; Lan, Minbo

doi:10.1166/jnn.2014.9192

Cited by 66 publications

(54 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[3][4][5] The paramagnetic metal ions present in magnetic contrast agents, possess permanent magnetic moments that contribute to strongly increase the local magnetic field in the vicinity of each ion. Through paramagnetic relaxation they produce a decrease of spin lattice (T 1 ) and spin-spin (T 2 ) relaxation times of neighboring hydrogen nuclei, a phenomenon called "proton relaxation enhancement (PRE)".…”

Section: Introductionmentioning

confidence: 99%

¹H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

et al. 2015

View full text Add to dashboard Cite

A proton nuclear magnetic relaxation dispersion (1)H NMRD study of the molecular dynamics in mixtures of magnetic ionic liquid [P66614][FeCl4] with [P66614][Cl] ionic liquid and mixtures of [P66614][FeCl4] with dimethyl sulfoxide (DMSO) is presented. The proton spin-lattice relaxation rate, R1, was measured in the frequency range of 8 kHz-300 MHz. The viscosity of the binary mixtures was measured as a function of an applied magnetic field, B, in the range of 0-2 T. In the case of DMSO/[P66614][FeCl4] the viscosity was found to be independent from the magnetic field, while in the case of the [P66614][Cl]/[P66614][FeCl4] system viscosity decreased with the increase of the magnetic field strength. The spin-lattice relaxation results were analyzed for all systems taking into account the relaxation mechanisms associated with the molecular motions with correlation times in a range between 10(-11) and 10(-7)s, usually observed by NMRD, and the paramagnetic relaxation contributions associated with the presence of the magnetic ions in the systems. In the case of the DMSO/[P66614][FeCl4] system the R1 dispersion shows the relaxation enhancement due to the presence of the magnetic ions, similar to that reported for contrast agents. For the [P66614][Cl]/[P66614][FeCl4] system, the R1 dispersion presents a much larger paramagnetic relaxation contribution, in comparison with that observed for the DMSO/[P66614][FeCl4] mixtures but different from that reported for other magnetic ionic liquid system. In the [P66614][Cl]/[P66614][FeCl4] system the relaxation enhancement associated with the paramagnetic ions is clearly not proportional to the concentration of magnetic ions, in contrast with what is observed for the DMSO/[P66614][FeCl4] system.

show abstract

Section: Introductionmentioning

confidence: 99%

¹H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Recently, nanotechnologies have contributed to advances in in vivo high-resolution imaging to monitor stem cells and exosomes tracking and homing, providing useful insights in their mechanism of action. [18][19][20][21][22][23] To produce a detectable change in signal intensity, cells or exosomes must be labeled with magnetic resonance (MR) contrast agents. In this regard, superparamagnetic iron oxide nanoparticles, which are small crystalline magnetite structures ranging in size from 5 nm to 150 nm, have been widely used to magnetically label stem cells and exosomes.…”

mentioning

confidence: 99%

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Busato

Bonafede

Bontempi

et al. 2016

IJN

View full text Add to dashboard Cite

Purpose Recent findings indicate that the beneficial effects of adipose stem cells (ASCs), reported in several neurodegenerative experimental models, could be due to their paracrine activity mediated by the release of exosomes. The aim of this study was the development and validation of an innovative exosome-labeling protocol that allows to visualize them with magnetic resonance imaging (MRI). Materials and methods At first, ASCs were labeled using ultrasmall superparamagnetic iron oxide nanoparticles (USPIO, 4–6 nm), and optimal parameters to label ASCs in terms of cell viability, labeling efficiency, iron content, and magnetic resonance (MR) image contrast were investigated. Exosomes were then isolated from labeled ASCs using a standard isolation protocol. The efficiency of exosome labeling was assessed by acquiring MR images in vitro and in vivo as well as by determining their iron content. Transmission electron microscopy images and histological analysis were performed to validate the results obtained. Results By using optimized experimental parameters for ASC labeling (200 µg Fe/mL of USPIO and 72 hours of incubation), it was possible to label 100% of the cells, while their viability remained comparable to unlabeled cells; the detection limit of MR images was of 10 2 and 2.5×10 3 ASCs in vitro and in vivo, respectively. Exosomes isolated from previously labeled ASCs retain nanoparticles, as demonstrated by transmission electron microscopy images. The detection limit by MRI was 3 µg and 5 µg of exosomes in vitro and in vivo, respectively. Conclusion We report a new approach for labeling of exosomes by USPIO that allows detection by MRI while preserving their morphology and physiological characteristics.

show abstract

“…Coating substrates include dextran, carboxydextran, polyethylene glycol (PEG), polystyrene and silica [77] . Iron oxide nanoparticles can be subdivided into standard SPION, 60 to 150 nm in size, and ultrasmall superparamagnetic iron oxide nanoparticles (USPION) which measure 10 to 20 nm [78,79] . The type of coating, size and method of synthesis affect the SPIONs biocompatibility and magnetic properties [80] .…”

Section: Cell Labeling For Mrimentioning

confidence: 99%

Mesenchymal stem cell tracking in the intervertebral disc

Handley

Goldschlager

Oehme

et al. 2015

WJSC

View full text Add to dashboard Cite

Low back pain is a common clinical problem, which leads to significant social, economic and public health costs. Intervertebral disc (IVD) degeneration is accepted as a common cause of low back pain. Initially, this is characterized by a loss of proteoglycans from the nucleus pulposus resulting in loss of tissue hydration and hydrostatic pressure. Conservative management, including analgesia and physiotherapy often fails and surgical treatment, such as spinal fusion, is required. Stem cells offer an exciting possible regenerative approach to IVD disease. Preclinical research has demonstrated promising biochemical, histological and radiological results in restoring degenerate IVDs. Cell tracking provides an opportunity to develop an in-depth understanding of stem cell survival, differentiation and migration, enabling optimization of stem cell treatment. Magnetic Resonance Imaging (MRI) is a non-invasive, non-ionizing imaging modality with high spatial resolution, ideally suited for stem cell tracking. Furthermore, novel MRI sequences have the potential to quantitatively assess IVD disease, providing an improved method to review response to biological treatment. Superparamagnetic iron oxide nanoparticles have been extensively researched for the purpose of cell tracking. These particles are biocompatible, non-toxic and act as excellent MRI contrast agents. This review will explore recent advances and issues in stem cell tracking and molecular imaging in relation to the IVD.

show abstract

Ultrasmall Superparamagnetic Iron Oxide Nanoparticles for Magnetic Resonance Imaging Contrast Agent

Cited by 66 publications

References 0 publications

¹H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

¹H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Mesenchymal stem cell tracking in the intervertebral disc

Contact Info

Product

Resources

About

Ultrasmall Superparamagnetic Iron Oxide Nanoparticles for Magnetic Resonance Imaging Contrast Agent

Cited by 66 publications

References 0 publications

1H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

1H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

Magnetic resonance imaging of ultrasmall superparamagnetic iron oxide-labeled exosomes from stem cells: a new method to obtain labeled exosomes

Mesenchymal stem cell tracking in the intervertebral disc

Contact Info

Product

Resources

About

¹H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent

¹H NMR Relaxation Study of a Magnetic Ionic Liquid as a Potential Contrast Agent