Ultrasmall gold nanoparticles for highly specific isolation/enrichment of N-linked glycosylated peptides

Tran, Trang Huyen; Park, Sunyoung; Lee, Hyunjin; Park, Sungsuk; Kim, Bora; Kim, Ok Hee; Oh, Byung‐Chul; Lee, Hookeun

doi:10.1039/c1an15810d

Cited by 27 publications

(21 citation statements)

References 22 publications

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“…Besides proteins, metal NCs were also applied for detection of other important biomolecules. For example, Tran et al reported the first method to use the ultrasmall Au NCs in isolation/enrichment of N ‐glycosylated peptides. Hydrazide‐functionalized Au NCs exhibited a large capacity for glycoproteins capturing.…”

Section: Metal Ncs For Biosensor Developmentmentioning

confidence: 99%

Ultrasmall metal nanoclusters for bio‐related applications

Tan

Jin

2013

WIREs Nanomed Nanobiotechnol

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The study of ultrasmall metal nanoclusters (NCs, ranging from subnanometer to ca 2 nm) is evidently a quickly evolving field in current nanoscience and nanotechnology research. Metal NCs, typically composed of several to hundreds of metal atoms, have attracted great interest in recent years owing to their unique properties including ultrasmall size and enhanced photoluminescence, together with other properties such as excellent photostability, low toxicity, and good biocompatibility desired for biological applications. This review summarizes recent advances in the field of bio-related applications of metal NCs materials. We highlight the applications of metal NCs for biosensor development, fluorescent biological imaging, and biomedical research, and finally discuss briefly some current challenges and future work.

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Section: Metal Ncs For Biosensor Developmentmentioning

confidence: 99%

Ultrasmall metal nanoclusters for bio‐related applications

Tan

Jin

2013

WIREs Nanomed Nanobiotechnol

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“…Hydrazide‐functionalised AuNPs were found to be very stable in biological samples (Fig. ) . In addition, they exhibit unique solubility and large capacity for the capture of peptides, demonstrating great potential for the capture of target N ‐linked deglycosylated peptides.…”

Section: Discussionmentioning

confidence: 99%

“…Schematic illustration of glycopeptide capture using A u 25 NP . (A) Hydrazide functionalisation of glutathione‐protected A u 25 NP . (B) Glycopeptide‐capturing procedure.…”

Section: Methods Based On Other Separation Techniquesmentioning

confidence: 99%

Stationary phases for the enrichment of glycoproteins and glycopeptides

et al. 2014

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The analysis of protein glycosylation is important for biomedical and biopharmaceutical research. Recent advances in LC-MS analysis have enabled the identification of glycosylation sites, the characterisation of glycan structures and the identification and quantification of glycoproteins and glycopeptides. However, this type of analysis remains challenging due to the low abundance of glycopeptides in complex protein digests, the microheterogeneity at glycosylation sites, ion suppression effects and the competition for ionisation by co-eluting peptides. Specific sample preparation is necessary for comprehensive and site-specific glycosylation analyses using MS. Therefore, researchers continue to pursue new columns to broaden their applications. The current manuscript covers recent literature published from 2008 to 2013. The stationary phases containing various chemical bonding methods or ligands immobilisation strategies on solid supports that selectively enrich N-linked or sialylated N-glycopeptides are categorised with either physical or chemical modes of binding. These categories include lectin affinity, hydrophilic interactions, boronate affinity, titanium dioxide affinity, hydrazide chemistry and other separation techniques. This review should aid in better understanding the syntheses and physicochemical properties of each type of stationary phases for enriching glycoproteins and glycopeptides.

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“…For example, the match in the size of these NPs to proteins [6] , multi helix DNA bundle structures [7] , and nuclear pores [8] is ideal for engineering biomolecule-NP interactions and targeting the cell nucleus; their high biocompatibility is maintained even after they are endocytosed by cells [9] ; their large surface-to-volume ratio provides high payload efficiency [10] ; and their strong diffusivity enables them to penetrate the blood–brain barrier [11] and solid tumor tissues [12] . These properties make sAuNPs excellent vehicles for various applications including biosensor [13] , [14] , [15] , drug/gene delivery [16] , [17] , [18] , and cancer therapy [19] .…”

Section: Methods Detailsmentioning

confidence: 99%