Ultrasensitive and specific multi-miRNA detection based on dual signal amplification

Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and practical applications

“…123–125 It is considered as a new tumor marker for rapid, sensitive, and accurate detection of miRNAs, and has high practical significance and value. In order to increase the sensitivity of miRNA detection, nucleic acid amplification methods are frequently employed, such as RCA, 126,127 duplex-specific nucleases (DSN)-based amplification, 128,129 LAMP, 130,131 exponential amplification reaction, 132,133 SDA 134,135 and some enzyme-free amplification. 136,137 As a new nucleic acid amplification technique, RCA is well appropriate for signal amplification that not only provides the sensibility and specificity of analysis but also keeps the multiplexing of stereo-analysis.…”

Gold nanoparticle-decorated covalent organic frameworks as amplified light-scattering probes for highly sensitive immunodetection of Salmonella in milk

“…Therefore, great efforts have been made to improve the sensitivity of immunoassays, especially through applying high-sensitivity signal transduction systems and integrating signal amplification techniques. 12,13 Dynamic light scattering (DLS) is a powerful tool for determining the size distribution of nano-or micro-sized particles based on their light scattering signals. 14 In recent years, immunosensors that utilize DLS-based signal transduction have received increasing attention because of their unique merits of high sensitivity, simple operation, and rapid data collection.…”

“…Therefore, great efforts have been made to improve the sensitivity of immunoassays, especially through applying high-sensitivity signal transduction systems and integrating signal amplification techniques. 12,13…”

Simple Amplifier Coupled with a Lanthanide Labeling Strategy for Multiplexed and Specific Quantification of MicroRNAs

“…Recent advances in analytical methodology and biological technology have made a great contribution to the development of various bioassays to characterize miRNA profiles, such as fluorescence-based, − electrochemical-based, , and Raman-based , bioassays. These methods involve different tags of fluorophores, electrochemical molecules, and Raman dyes for multiple miRNA detection.…”