Ultrasensitive and multiplex detection of four pathogenic bacteria on a bi-channel lateral flow immunoassay strip with three-dimensional membrane-like SERS nanostickers

Nanogapped Fe3O4@Au Surface-Enhanced Raman Scattering Tags for the Multiplex Detection of Bacteria on an Immunochromatographic Strip

“…In theory, by changing the detection antibodies or using other recognition molecules (e.g., peptides, aptamers, and antibiotics) for bacteria, the proposed Mag@Au SERS-ICA has the potential to detect more types of pathogens with high sensitivity and selectivity. Notably, some recent researches show that the combination of different Raman-reporter-molecule-modified SERS tags and a single test line can greatly improve the multiplex detection ability of the ICA strip and simplifies the measurement process of SERS signals. − In the future, we will integrate different Raman-reporter-molecule-modified Mag@Au tags into a single test-line-based ICA strip to further broaden the application potential of the SERS-ICA technique.…”

Introduction of graphene oxide-supported multilayer-quantum dots nanofilm into multiplex lateral flow immunoassay: A rapid and ultrasensitive point-of-care testing technique for multiple respiratory viruses

“…S2 in the ESM. The CdSe/ZnS-MPA QDs are widely used to build QD-based fluorescent microspheres (e.g., SiO 2 @QD and Fe 3 O 4 @QD) for LFA methods [35][36][37]. The structure and surface morphology of the GO-based QD nanofilms were characterized through transmission electron microscopy (TEM) and scanning electron microscopy (SEM).…”

Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags

“…However, high-affinity antibodies are difficult to prepare, easily denatured, and subject to poor reproducibility, which greatly increase the difficulty of LFA construction. [13][14][15][16] In addition, the complex matrixes (e.g., high sugar, high salt, and low pH) in actual food or environmental samples easily affect the stability of common LFA methods, thus easily causing falsepositive results or misdiagnosis. [17][18][19][20] Until now, a sensitive and universal LFA technique for different bacteria detection that can be directly used in complex samples has not been reported.…”