Ultramorphology of the peritrophic matrix in bees (Hymenoptera: Apidae)

Teixeira, Aparecida das Dores; Marques-Araújo, Solange; Zanúncio, José Cola; Serrão, José Éduardo

doi:10.1080/00218839.2019.1584961

Cited by 8 publications

(6 citation statements)

References 40 publications

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“…The striated border of the midgut increases the cell surface for the transport of substances [ 56 , 57 , 58 ], whereas the luminal peritrophic matrix is a physical barrier against mechanical injuries, pathogens and xenobiotic agents, and controls digestion [ 58 , 59 ]. The peritrophic matrix has its components (chitin and proteins) produced by the digestive cells and released by the microvilli of these cells [ 60 , 61 , 62 ]. Thus, the damage in these microvilli and in the peritrophic matrix formation may compromise the digestive process in S. frugiperda caterpillars.…”

Section: Discussionmentioning

confidence: 99%

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Vinha

Plata-Rueda

Soares

et al. 2021

Insects

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Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is the main pest of maize crops, and effective methods for pest management are needed. The insecticidal efficacy of deltamethrin was evaluated against S. frugiperda for toxicity, survival, locomotion, anti-feeding, and histological changes in the midgut. Concentration–mortality bioassays confirmed that deltamethrin (LC50 = 3.58 mg mL−1) is toxic to S. frugiperda caterpillars. The survival rate was 99.7% in caterpillars not exposed to deltamethrin, decreasing to 50.3% in caterpillars exposed to LC50, and 0.1% in caterpillars treated with LC90. Spodoptera frugiperda demonstrated reduced mobility on deltamethrin-treated surfaces. Deltamethrin promoted a low respiration rate of S. frugiperda for up to 3 h after insecticide exposure, displaying immobilization and inhibiting food consumption. Deltamethrin induces histological alterations (e.g., disorganization of the striated border, cytoplasm vacuolization, and cell fragmentation) in the midgut, damaging the digestive cells and peritrophic matrix, affecting digestion and nutrient absorption.

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Section: Discussionmentioning

confidence: 99%

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Vinha

Plata-Rueda

Soares

et al. 2021

Insects

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“…In the honey bee A. mellifera , the peritrophic matrix is type I, with gelatinous consistency and several layers that present morphological differences, according to the location. They are thicker when close to the ectoperitrophic space (space between the peritrophic matrix and the epithelium) and thinner, when close to the endoperitrophic space (Teixeira et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The peritrophic matrix delays Nosema ceranae infection in the honey bee Apis mellifera midgut

Oliveira

Souza

Resende

et al. 2022

Physiological Entomology

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The midgut of bees plays a significant role in digestion and absorption and is a gateway for pathogens. As a protection for the organism, the midgut lumen is lined by a peritrophic matrix rich in chitin fibrils, associated with proteins that form multiple resistant layers with gelatinous consistency, with several functions, including the mechanical barrier against infection by pathogens. The obligate intracellular microsporid Nosema ceranae is an important pathogen that decreases honey production, increases pesticide susceptibility, and may cause mortality in the honey bee Apis mellifera. This study evaluated whether the peritrophic matrix has a protective function against infection by N. ceranae in A. mellifera adult workers. The peritrophic matrix integrity was changed by feeding the bees with calcofluor white (CFW), followed by an artificial infection with N. ceranae spores. Results shown that 0.5% CFW affect the integrity of the peritrophic matrix with areas of interruption of this envelope. This disorganization of the peritrophic matrix reduces the life cycle period of N. ceranae from eight to four days, after inoculation, and increases the concentration of new spores released in the midgut lumen. This is the first report on the importance of the peritrophic matrix as a protective barrier against N. ceranae infection in A. mellifera.

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“…The main roles of the PM include not only preventing the damage of epithelial cells by the luminal contents, but also protecting them from pathogenic microorganisms and toxins. It is also known that while it is permeable to inorganic ions and small organic molecules, it is a barrier to the passage of large molecules (Pimenta et al, 1997;Dias et al, 2019;Teixeira et al, 2019;Nayak, 2020). In this study, the peritrophic membrane is observed through the whole midgut in a similar manner to that of G. gratiosa (Li et al, 2018), A. domesticus (Thorsson, 2018), Acrida anatolica Dirsh, 1949 (Orthoptera, Acrididae), Parapholidoptera spinulosa Karabag, 1956 (Orthoptera, Tettigoniidae) (Eren et al, 2019), and I. nervosa (Amutkan Mutlu, 2020).…”

Section: Resultsmentioning

confidence: 99%

Morphology and Fine Organization of the Midgut of Poecilimon ataturki Ünal, 1999 (Orthoptera: Tettigoniidae)

Mutlu

2021

Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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In order to do detailed experiments about the internal organs of the insects or to improve new methods relevant to pest management, the biology of the species should be well known. For this reason, this paper deals with Poecilimon ataturki Ünal, 1999 (Orthoptera: Tettigoniidae) which is a bush cricket and is known to be harmful to nature. The morphology and structure of the midgut of P. ataturki have been investigated using light microscopy and scanning electron microscopy (SEM). The midgut of this species has two main regions called the ventriculus and the gastric caecum. The ventriculus has a tubular structure morphologically, whereas the gastric caecum has a bulbous structure. The ventriculus has two cell types in the epithelial layer as the principle and the regenerative cells. The fine structure of the ventriculus and the gastric caecum cells revealed that the secretion activity moves from cell to lumen. These structures show a high level of similarity compared with the midgut structure of the previously studied species in the Orthoptera order despite some differences. It is thought that the enlightenment of the midgut structure of this species will contribute to science for insect pest management.

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Ultramorphology of the peritrophic matrix in bees (Hymenoptera: Apidae)

Cited by 8 publications

References 40 publications

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

The peritrophic matrix delays Nosema ceranae infection in the honey bee Apis mellifera midgut

Morphology and Fine Organization of the Midgut of Poecilimon ataturki Ünal, 1999 (Orthoptera: Tettigoniidae)

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