We present a simple yet elegant Mueller matrix approach for controlling the Fano interference effect and engineering the resulting asymmetric spectral line shape in anisotropic optical system. The approach is founded on a generalized model of anisotropic Fano resonance, which relates the spectral asymmetry to two physically meaningful and experimentally accessible parameters of interference, namely, the Fano phase shift and the relative amplitudes of the interfering modes. The differences in these parameters between orthogonal linear polarizations in an anisotropic system are exploited to desirably tune the Fano spectral asymmetry using pre-and post-selection of optimized polarization states. We begin with a phenomenological model where a Fano-type spectral asymmetry in the scattered intensity naturally arises due to the interference of the complex Lorentzian field ( ( )) of a narrow resonance with a broad spectrum of relative field amplitude ( ) assumed to be independent of frequency (ideal continuum):Here, = ( ) = âThe resulting expression for the scattered intensity becomes The first term represents the Fano-type asymmetric line shape with an effective asymmetry parameter = â . The second term corresponds to a Lorentzian background, widely reported in Fano resonance from diverse optical systems [6,25]. It is