Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases

Klejdus, Bořivoj; Vacek, Jan; Lojková, Lea; Benešová, Lenka; Kubáň, Vlastimil

doi:10.1016/j.chroma.2008.04.069

Cited by 69 publications

(32 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the most widely used methods are based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with UV-Vis detection and/or mass spectrometry (Abad-García et al, 2007;Klejdus, Vacek, Lojková, Benešová, & Kubáň , 2008;Seeram, Lee, Scheuller, & Heber, 2006;Shui & Leong, 2002).…”

Section: Introductionmentioning

confidence: 99%

Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

Saraji

Mousavi

2010

Food Chemistry

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

Saraji

Mousavi

2010

Food Chemistry

View full text Add to dashboard Cite

“…Many analytical methods have been described for determination of flavonols in wines. The most selective methods involve often liquid or gas chromatography coupled to detection by mass or tandem mass spectrometry [35][36][37][38][39][40][41][42][43][44][45][46][47][48].…”

Section: Introductionmentioning

confidence: 99%

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

Silva

Gonçalves

Câmara

2012

Analytica Chimica Acta

View full text Add to dashboard Cite

“…In vitro plant regeneration in natural tetraploid T. pratense has been done by Colgecen and Toker (2008). However, various isoflavone analysis studies were made on the diploid T. pratense (Kledjus et al, 2001;Krenn et al, 2002;Wu et al, 2003;Delmonte et al, 2006) and other Fabaceae species (Kennely et al, 2002;Wang et al, 2002;Kledjus et al, 2004;Kledjus et al, 2005;Jiang et al, 2006;Kledjus et al, 2008). such a study was not done in the natural tetraploid form.…”

Section: Introductionmentioning

confidence: 99%

In vitro isoflavonoid production and analysis in natural tetraploid Trifolium pratense (red clover) calluses

Erçetin¹,

Toker²,

Kartal³

et al. 2012

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Isoflavones are polyphenolic phytoestrogens, predominantly found in leguminous plants. Trifolium pratense L., Fabaceae (red clover), is rich in isoflavones that possess estrogenic activity due to their similar molecular structure and effectiveness in preventing health conditions such as menopause, osteoporosis, cardiovascular disease, hypertension and hormone-dependent cancers. In this study, presence and amount of various phytoestrogens in the tetraploid plant and in the calluses derived from the plants were investigated. Calluses were generated from explants obtained from natural tetraploid T. pratense seedlings. The best callus formation was obtained from hypocotyl explants cultured in Phillips Collins and Gamborg B5 media containing different plant growth regulators. Flowers of plants and calluses were analysed for formononetin, biochanin A, genistein and daidzein contents using HPLC. In HPLC analysis, high levels of formononetin (0.249 µg/mg) were determined in natural tetraploid T. pratense flowers in addition to genistein and biochanin A. In calluses, highest isoflavone content (1.15 µg/mg formononetin) was observed in modified Gamborg B5 medium. Biochanin A content of calluses and the plant were found to be nearly the same. But formononetin and genistein contents of the calluses in this medium were found to be respectively 4.62 and 21.39 folds higher than the tetraploid plant.

show abstract

Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases

Cited by 69 publications

References 39 publications

Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

Use of hollow fibre-based liquid–liquid–liquid microextraction and high-performance liquid chromatography–diode array detection for the determination of phenolic acids in fruit juices

A sensitive microextraction by packed sorbent-based methodology combined with ultra-high pressure liquid chromatography as a powerful technique for analysis of biologically active flavonols in wines

In vitro isoflavonoid production and analysis in natural tetraploid Trifolium pratense (red clover) calluses

Contact Info

Product

Resources

About