Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities

Cruz, Daniela F.; Fontes, Cassio M.; Semeniak, Daria; Huang, Jiani; Hucknall, Angus; Chilkoti, Ashutosh; Mikkelsen, Maiken H.

doi:10.1021/acs.nanolett.0c01051

Cited by 33 publications

(34 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Sefmentioning

confidence: 99%

“…[203] Cruz et al achieved a NPoM nanogap structure by integrating Ag nanocubes and a Au film into a sandwich immunoassay microarray to detect B-type natriuretic peptide, a cardiac biomarker (Figure 7d). [297] The plasmon resonance wavelength overlapped with the absorption and emission band of the fluorophores. The fluorophore-modified detection antibody was positioned inside the nanogap, where increased local electromagnetic fields exist, and exhibited 151-fold enhanced fluorescence compared to the glass control.…”

Section: Sefmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Synthesis, Assembly, Optical Properties, and Sensing Applications of Plasmonic Gap Nanostructures

Kim

Lee

et al. 2021

Advanced Materials

View full text Add to dashboard Cite

sharp-tip structures with a highly focused field at the end of the tip. [7] In particular, plasmonic gap nanostructures (PGNs) are among the most promising materials for diverse applications with highly localized field and amplified optical signals in the gap including sensing, spectroscopy, optics, biomedicine, electronics, and energy applications. [8][9][10][11][12][13] The development of bottom-up or top-down synthetic methods enabled the access to numerous nanogap structures with diverse gap sizes, morphologies, and compositions. [14][15][16][17][18] The highly localized electric field inside the plasmonic nanogap and the appearance of plasmonic coupling in closely neighboring structures have been studied, leading to the discovery of interesting optical phenomena and advances in sensing such as ultrasensitive spectroscopy, singlemolecule sensing methods, and in situ detection techniques. [19][20][21] As small changes in the distance, morphology, and composition of the nanogap lead to significant variations in the optical responses, the highly precise, controllable, and high-yielding preparation of PGNs is important to achieve reproducible and reliable results. However, although numerous pioneering studies on basic synthetic methods, characteristics, and applications enabled the understanding and control over plasmonic nanogaps, key challenges still remain for the practical use and applications of PGNs. In particular, the sub-nanometer, atomic-level control of the nanogap, coupled with the scaling-up issue, and its fundamental understanding have not yet been fully accomplished. For example, in surfaceenhanced Raman spectroscopy (SERS) using plasmonic gaps, early research focused on a high enhancement factor (EF). In contrast, recent studies revealed that a deep understanding at atomic scale is required, as differences in the small regime such as picocavities or molecular orientation can have a large effect on the signal. [22][23][24] Hence, a comprehensive understanding of the nanogap is critical to the further development of the field.This progress report addresses emerging issues and challenges in the field of nanogap-based plasmonics and plasmonic nanomaterials. We first describe the challenges in the creation of intra-or intergaps by bottom-up or top-down synthesis, and discuss breakthroughs that allow for overcoming these obstacles. Next, optical properties according to gap size, morphology, and composition are summarized to provide insight into the nature of gap plasmonics. The discussion of applications in this article mainly highlights surface-enhanced Plasmonic gap nanostructures (PGNs) have been extensively investigated mainly because of their strongly enhanced optical responses, which stem from the high intensity of the localized field in the nanogap. The recently developed methods for the preparation of versatile nanogap structures open new avenues for the exploration of unprecedented optical properties and development of sensing applications relying on the amplification of various optical signals....

show abstract

Section: Sefmentioning

confidence: 99%

Section: Sefmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, Assembly, Optical Properties, and Sensing Applications of Plasmonic Gap Nanostructures

Kim

Lee

et al. 2021

Advanced Materials

View full text Add to dashboard Cite

show abstract

“…Because of their inherent antibiofouling nature, the fabrication of microarrays on the polymer brush modified substrates allows biomolecular immobilization and recognition with low nonspecific interactions, resulting in a significant improvement of the specificity and reproducibility of microarray [77][78][79][80][81][82][83][84][85][86][87][88][89][90][91][92][93][94][95]. In addition, the 3D structure of polymer brush modified substrates provide a high biomolecule immobilization capacity and accessible scaffolds with sufficient space for biomolecule binding, leading to an increase in the sensitivity of microarrays.…”

Section: Polymer Brush-based Microarraysmentioning

confidence: 99%

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

View full text Add to dashboard Cite

Polymers with different structures and morphology have been extensively used to construct functionalized surfaces for a wide range of applications because the physicochemical properties of polymers can be finely adjusted by their molecular weights, polydispersity and configurations, as well as the chemical structures and natures of monomers. In particular, the specific functions of polymers can be easily achieved at post-synthesis by the attachment of different kinds of active molecules such as recognition ligand, peptides, aptamers and antibodies. In this review, the recent advances in the bioanalytical and biomedical applications of polymer modified substrates were summarized with subsections on functionalization using branched polymers, polymer brushes and polymer hydrogels. The review focuses on their applications as biosensors with excellent analytical performance and/or as nonfouling surfaces with efficient antibacterial activity. Finally, we discuss the perspectives and future directions of polymer modified substrates in the development of biodevices for the diagnosis, treatment and prevention of diseases.

show abstract

“…Over the last decade, μPADs and similar immunoassays have shown great promise as diagnostic methodologies in POC and low-resource settings due to the variety of production methods that can be employed in manufacturing them, as well as the wide array of biomarkers that can be utilized for detection, similar to biosensors [ 31 , 119 , 120 ]. Applications have also been found in high-throughput drug screening and environmental monitoring [ 120 , 121 ], colorimetric measurement of proteins in urine [ 122 ], and even plasmonically enhanced immunoassays utilizing synthetic polymers in place of cellulose [ 123 ].…”

Section: Integrated and Portable Sample Preparation Devicesmentioning

confidence: 99%

Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review

Nichols

Geddes

2021

Molecules

View full text Add to dashboard Cite

Sample preparation is an essential step for nearly every type of biochemical analysis in use today. Among the most important of these analyses is the diagnosis of diseases, since their treatment may rely greatly on time and, in the case of infectious diseases, containing their spread within a population to prevent outbreaks. To address this, many different methods have been developed for use in the wide variety of settings for which they are needed. In this work, we have reviewed the literature and report on a broad range of methods that have been developed in recent years and their applications to point-of-care (POC), high-throughput screening, and low-resource and traditional clinical settings for diagnosis, including some of those that were developed in response to the coronavirus disease 2019 (COVID-19) pandemic. In addition to covering alternative approaches and improvements to traditional sample preparation techniques such as extractions and separations, techniques that have been developed with focuses on integration with smart devices, laboratory automation, and biosensors are also discussed.

show abstract

Ultrabright Fluorescence Readout of an Inkjet-Printed Immunoassay Using Plasmonic Nanogap Cavities

Cited by 33 publications

References 40 publications

Synthesis, Assembly, Optical Properties, and Sensing Applications of Plasmonic Gap Nanostructures

Synthesis, Assembly, Optical Properties, and Sensing Applications of Plasmonic Gap Nanostructures

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sample Preparation and Diagnostic Methods for a Variety of Settings: A Comprehensive Review

Contact Info

Product

Resources

About