Ultra-sensitive and selective determination of a phenolic food additive using protein capped gold nanoclusters: a dual in-line fluorometric and colorimetric sensing probe

Shankar, Sekar; Gowthaman, N.S.K.; Arul, P.; Chen, Feng; Lim, Hong Ngee; Qin, Fengxiang

doi:10.1039/d0nj04712k

Cited by 13 publications

(9 citation statements)

References 43 publications

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“…Recently, the colorimetric detection of various other food additives such as azodicarbonamide (ADA) and sodium benzoate was also reported using nanomaterials. 121,122 For example, a MnO 2 nanosheet-3,3′,5,5′-tetramethylbenzidine-glutathione (MnO 2 -TMB-GSH) based oxidative detection system was devel-oped for the detection of ADA in flour samples (Fig. 2).…”

Section: Colorimetric Sensorsmentioning

confidence: 99%

“…In the presence of interferents, the results demonstrated that the suggested approach was highly selective for TBHQ (ranging from 2.5 to 50 mM) with a LOD of 72 nM. 122 In another study, organic NPs based on a tetraphenyl imidazole compound (TIBM) were employed for the development of a "TURN-ON" sensor to detect sulfite (a food preservative) within 15 seconds. 124 The analyte was detected via aggregation-induced emission enhancement (AIEE) in the presence of sulfite.…”

Section: Fluorescent Sensorsmentioning

confidence: 99%

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Recent advancements in nanomaterial based optical detection of food additives: a review

Bhardwaj,

Deep,

Bhardwaj

et al. 2023

Analyst

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Section: Colorimetric Sensorsmentioning

confidence: 99%

Section: Fluorescent Sensorsmentioning

confidence: 99%

Recent advancements in nanomaterial based optical detection of food additives: a review

Bhardwaj,

Deep,

Bhardwaj

et al. 2023

Analyst

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“…If Au NCs were prepared using bovine serum albumin (BSA) as protective ligands, 33 the removal of BSA ligands leads to changes in the properties of BSA−Au NCs. Trypsin is an endopeptidase that could hydrolyze arginine and lysine peptide chains in BSA molecules, 34 so trypsin can hydrolyze BSA ligands on the surface of BSA−Au NCs.…”

Section: ■ Introductionmentioning

confidence: 99%

Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids

et al. 2023

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The detection of trypsin is significantly important for both clinical diagnosis and disease treatment. In this study, an innovative multicolor sensor for trypsin detection has been established based on the regulation of the peroxidase activity of bovine serum albumin-coated gold nanoclusters (BSA−Au NCs) and efficient etching of gold nanobipyramids (Au NBPs). BSA−Au NCs have slight peroxidase enzyme activity and can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate TMB + , while trypsin can hydrolyze BSA ligands on the surface of BSA−Au NCs, thus exposing more catalytic active sites of BSA−Au NCs and resulting in the enhancement of the peroxidase activity of BSA−Au NCs, hence more TMB + is generated. Under acidic conditions, TMB + can etch Au NBPs efficiently, consequently affecting the aspect ratio of Au NBPs accompanied by the ultraviolet−visible (UV−vis) spectra blue shifting of the system. Furthermore, this also results in color variations that can be distinguished and recognized by naked eyes without any expensive and sophisticated instruments. This multicolor sensor has an available linear relationship with the logarithm of the trypsin concentration in the range of 0.1−100 μg/mL, and the detection limit is 0.045 μg/mL. The designed sensor has been used to detect the concentration of trypsin in human serum samples from healthy individuals and pancreatitis patients with satisfactory results.

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“…So far, great advances have been got in the quantitative research and analysis of TBHQ. Common analysis methods for the determination of TBHQ include high-performance liquid chromatography, gas chromatography–mass spectrometry, electrochemical methods, fluorimetric detection, and capillary electrophoresis. − Among the above methods, the fluorescence analysis method is considered to be the most prospective technology due to its high sensitivity and selectivity . Moreover, fluorescence detection is simple, time-saving, low cost, and convenient; thus, fluorescent sensors could be potential candidates for monitoring TBHQ .…”

Section: Introductionmentioning

confidence: 99%

Novel Dual-Emission Carbonized Polymer Dot-Based Ratiometric Fluorescence Probe for the Sensitive Detection of Tertiary Butylhydroquinone

Xu,

Yang,

Liu

2023

ACS Appl. Mater. Interfaces

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Tertiary butylhydroquinone (TBHQ), one of the most common antioxidants in many foods, has attracted widespread attention due to its potential risk to human health. In this work, an “on–off–on” ratiometric fluorescent probe was prepared using dual-emission carbonized polymer dots (d-CPDs), which can be used to detect TBHQ in edible oils. The ratiometric fluorescent sensing system consisted of blue fluorescent CPDs (b-CPDs) as the response signal and yellow fluorescent CPDs (y-CPDs) as the internal reference material. The blue fluorescence of b-CPDs was gradually quenched with increasing Fe3+ ion concentration, while the yellow fluorescence was essentially unaffected. Interestingly, TBHQ can restore the fluorescence intensity of b-CPDs. Meanwhile, the fluorescence mechanism of Fe3+ on b-CPDs was investigated by density functional theory, both after the addition of TBHQ, and CPDs were released and their fluorescence was restored due to the competitive reaction of TBHQ-Fe3+. Thus, the d-CPDs probe was able to detect Fe3+ accurately in an “on–off” manner and thus TBHQ in an “off–on” manner. At an optimal Fe3+ concentration, the ratiometric sensing system provided fine linearities for determining TBHQ in the range from 0.2 to 2 μM and an ingenious detection limit of 0.052 μM. The CPDs ratiometric fluorescent probe designed in this work may have a broader application in the rapid, susceptive, and low-cost determination of TBHQ in various foods.

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Ultra-sensitive and selective determination of a phenolic food additive using protein capped gold nanoclusters: a dual in-line fluorometric and colorimetric sensing probe

Abstract: The application of red luminescence BSA-AuNCs towards the selective determination of food additive tert-butylhydroquinone (TBHQ) was demonstrated by both fluorometric and colorimetric methods.

Cited by 13 publications

References 43 publications

Recent advancements in nanomaterial based optical detection of food additives: a review

Recent advancements in nanomaterial based optical detection of food additives: a review

Multicolor Biosensor for Trypsin Detection Based on the Regulation of the Peroxidase Activity of Bovine Serum Albumin-Coated Gold Nanoclusters and Etching of Gold Nanobipyramids

Novel Dual-Emission Carbonized Polymer Dot-Based Ratiometric Fluorescence Probe for the Sensitive Detection of Tertiary Butylhydroquinone

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