Ultra performance liquid chromatography tandem mass spectrometry method for the measurement of anandamide in human plasma

Lam, Patricia M.W.; Marczylo, Timothy H.; El-Talatini, Mona R.; Finney, M. S.; Nallendran, Vijaianitha; Taylor, Anthony H.; Konje, Justin C.

doi:10.1016/j.ab.2008.05.033

Cited by 91 publications

(85 citation statements)

References 26 publications

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“…Virodhamine, which has a retention time of 1.54 min using this system [17], and is a possible confounding contaminant of AEA measurement was not detected in any of the bio-matrices.…”

Section: Measurement Of Aea In Other Human Bio-matricesmentioning

confidence: 94%

“…Liquid-phase extractions (LPE) were performed according to Habayeb et al, (2004) with minor modifications [11,17]. To determine extraction efficiencies from plasma, serum or saline (2 ml), 1.25 pmol/ml AEA-d8 internal standard was added prior to thorough mixing on a desktop vortex.…”

Section: Liquid-phase Extraction Of Aea From Human Biomatricesmentioning

confidence: 99%

“…The cartridges were washed with 1 ml 40% aqueous methanol and AEA was eluted in 1 ml acetonitrile. The eluate was dried under a constant stream of nitrogen and reconstituted in 80 l acetonitrile and transferred to a HPLC sample vial ready for UPLC-MS/MS analysis as described previously [17].…”

Section: Liquid-phase Extraction Of Aea From Human Biomatricesmentioning

confidence: 99%

“…AEA and AEA-d8 quantification was achieved using an UPLC-MS/MS system comprising an Acquity UPLC in line with a Quattro Premier tandem mass spectrometer (Waters, Elstree, UK) as described previously [17]. In brief, separation was accomplished with an Acquity UPLC BEH C 18 (2.1 x 50 mm) column maintained at 40C.…”

Section: Quantification Of Aeamentioning

confidence: 99%

“…We recently reported a UPLC-MS/MS method for the measurement of AEA in plasma [17]. The major limitation in that method was the liquid-based lipid extraction step.…”

Section: Introductionmentioning

confidence: 99%

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A solid-phase method for the extraction and measurement of anandamide from multiple human biomatrices

Marczylo

Lam

Nallendran

et al. 2009

Analytical Biochemistry

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Running Title: Extraction of AEA from human bio-matrices Abbreviations: AEA, N-arachidonoylethanolamine (anandamide); AEA-d8, deuterated anandamide; LOD, limit of detection; LOQ, limit of quantification; LPE, liquid-phase extraction; SPE, solid-phase extraction; UPLC-MS/MS, ultra performance liquid chromatography tandem mass spectrometry.Keywords: Endocannabinoid, anandamide, solid phase extraction.The authors affirm that they have no conflicts of interest. samples from labouring and non-labouring women were analysed using both techniques no extraction method-dependent differences were observed.Consequently, we provide evidence for a robust SPE technique for the extraction of AEA from bio-matrices to replace the existing liquid extraction methods which is superior in terms of speed, extraction efficiency and sample size required.

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“…Virodhamine, which has a retention time of 1.54 min using this system [17], and is a possible confounding contaminant of AEA measurement was not detected in any of the bio-matrices.…”

Section: Measurement Of Aea In Other Human Bio-matricesmentioning

confidence: 94%

Section: Liquid-phase Extraction Of Aea From Human Biomatricesmentioning

confidence: 99%

Section: Liquid-phase Extraction Of Aea From Human Biomatricesmentioning

confidence: 99%

Section: Quantification Of Aeamentioning

confidence: 99%

“…We recently reported a UPLC-MS/MS method for the measurement of AEA in plasma [17]. The major limitation in that method was the liquid-based lipid extraction step.…”

Section: Introductionmentioning

confidence: 99%

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A solid-phase method for the extraction and measurement of anandamide from multiple human biomatrices

Marczylo

Lam

Nallendran

et al. 2009

Analytical Biochemistry

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Clinical Chemistry in Pregnancy

Taylor

Melford

Konje

2013

Encyclopedia of Life Sciences

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During pregnancy, a woman undergoes a multitude of normal physiochemical changes that are specific to pregnancy and with increasing frequency, challenged with pregnancy‐related conditions. Additionally, there are a number of problems affecting the unborn fetus that, in turn, affect normal maternal biochemistry, endocrinology and physiology. Furthermore, normal biochemical, hormonal and clinically relevant measurements for the nonpregnant state often no longer apply to the pregnant woman. This makes clinical laboratory measurement difficult and normal clinical diagnosis using nonpregnant values potentially dangerous, with inappropriate treatment (when it is not required) and no treatment (when it is required) being offered. Problems within early pregnancy, for example, spontaneous and recurrent miscarriage, thus become very complicated to predict or treat. Therefore, reexamining what clinical chemical tests are available and what is just emerging from the experimental laboratory (and thus becoming available in the standard clinical laboratory), should inform the unwary clinician. Key Concepts: ‘Normal’ pregnancy biochemical marker (Biomarker) concentrations fluctuate throughout pregnancy. No single maternal biomarker can be used to predict gestational disease. Both the concentrations of urinary, serum, plasma, salivary and amniotic fluid biomarkers and the timing of clinical sampling are critical in disease diagnosis. Many new biomarkers are emerging, which may help aid the prediction/diagnosis of pregnancy‐related conditions.

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Tricks and Tracks in the Identification and Quantification of Endocannabinoids

Lerner

Lutz

Bîndilă

2013

Encyclopedia of Life Sciences

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The endocannabinoid system serves pivotal roles in a diverse range of physiological and pathophysiological states, including behavior, pain, schizophrenia, obesity, Alzheimer disease, multiple sclerosis and cardiovascular disease. A number of endocannabinoids (eCBs) and their receptors have been characterised and identified in a plethora of biological matrices. The eCBs include N ‐arachidonoyl ethanolamine (anandamide), 2‐arachidonoyl glycerol, 2‐arachidonoyl glyceryl ether (noladin ether), O ‐arachidonoyl ethanolamine (virodhamine) and N ‐arachidonoyl dopamine. Advanced targeted mass spectrometry methods, particularly the selected reaction monitoring, has facilitated sensitive quantification of eCBs owing particularly to minimisation of the complex and abundant lipid matrix, invariably accompanying the eCBs in biological extracts. In this article, state‐of‐the‐art analytical workflows for eCBs extraction and mass‐spectrometry identification and quantification are concisely reviewed. Challenges and considerations on the isolation and analytical conditions to prevent artificial interferences in the measurement of eCBs concentration will be highlighted. Key Concepts: Endocannabinoids are lipid signalling molecules with pivotal role in a plethora of physiological and pathophysiological states, including anxiety, fear, learning and memory, pain etc . Imbalances in the ECS activity underlie various psychiatric, neurological, metabolic and immunological diseases, and thus ECS is emerging as an appealing therapeutic target. Quantitative profiling of endocannabinoids in various biological tissues is imperative to discover their site of action and determine the reference and altered concentration values. Chemical isomerisation of eCBs, as well as ex‐vivo degradation and synthesis can occur during biological matrix sampling and processing, leading to artificial changes in the endogenous levels of eCBs. Conditions for biological matrix sampling, storage, transportation and eCBs extraction methods have to be tailored and standardised with respect to biological matrix to achieve a reliable determination of reference and disease or pathological state‐related altered concentrations of eCBs in tissues and bodily fluids. LC–ESI/SRM is the method of choice for eCBs quantification owing to the increased selectivity, specificity, robustness, precision and accuracy of quantification. Validation and standardisation of LC/MS assays, biological matrix sampling and processing from tissues and bodily fluids are pivotal to allow comparison of eCB levels across studies and for prospective clinical research.

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Ultra performance liquid chromatography tandem mass spectrometry method for the measurement of anandamide in human plasma

Cited by 91 publications

References 26 publications

A solid-phase method for the extraction and measurement of anandamide from multiple human biomatrices

A solid-phase method for the extraction and measurement of anandamide from multiple human biomatrices

Clinical Chemistry in Pregnancy

Tricks and Tracks in the Identification and Quantification of Endocannabinoids

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