UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

Abstract

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

Kapasitas Stabilisasi Radikal Bebas dan Kelasi Ion Metal Ekstrak Air Daun Vernonia amygdalina Del.

“…Beberapa penelitian tentang potensi ekstrak daun afrika sebagai antioksidan telah dilakukan. Ekstrak fraksi n-heksan daun afrika dapat menstabilkan radikal DPPH dengan konsentrasi penghambatan 50% (IC50) sebesar 371,98 ppm (Fatimah dan Sundu, 2020), ekstrak metanol dan etanol daun afrika memiliki IC50 terhadap radikal DPPH masingmasing sebesar 175,021 ppm dan 3.289 ppm (Febrianti dkk., 2017;Nurlatifah dkk., 2018).…”

“…Kapasitas stabilisasi radikal bebas DPPH ekstrak air daun afrika relatif rendah dibandingkan dengan jenis ekstrak yang lain. Fraksi ekstrak n-Heksan dan metanol daun Agritechnology 3(2) 2020 ISSN: 2615-885X (cetak), 2620-4738 (online) DOI: https://doi.org/10.51310/agritechnology.v3i2.62 afrika memiliki kapasitas stabilisasi radikal sebesar 50% pada konsentrasi ekstrak masingmasing sebesar 0,04 dan 0,02% (Febrianti dkk., 2017;Fatimah dan Sundu, 2020) Kapasitas stabilisasi radikal bebas ekstrak daun afrika juga relatif lebih rendah dibandingkan dengan vitamin C dengan konsentrasi 60 mg/ml atau setara dengan 0,06% yaitu sebesar 84,74%. Hasil tersebut menunjukkan bahwa ekstrak air daun afrika kurang berpotensi sebagai sumber antioksidan alami dalam menstabilkan radikal bebas.…”

ANTIOXIDANT ACTIVITY OF THE COMBINATION OF AMBARELLA LEAVES (Spondias dulcis Parkinson) AND SOURSOP LEAVES (Annona muricata Linn) EXTRACT

“…Indonesia has a very abundant wealth of natural resources that can benefit the health sector; one example is an antioxidant. Antioxidants are used as free radical scavenging compounds (Fatimah & Sundu, 2020). Free radicals are unstable atoms or molecules with high reactivity so that they can cause damage to the body.…”

"Formulation and Evaluation of Effervescent Granul from African Leaf Extract (Vernonia amygdalina Del.) "

“…Vernonia amygdalina plant (Yeap et al, 2010) One of the plants used as a folk remedy is the African leaf plant (Vernonia amygdalina Del). African leaf or also known as bitter leaf (Fatimah & Sundu, 2020), is a plant that contains nutrients and chemical compounds that are used in traditional medicine and can be used for various diseases, such as cancer drugs, heart disease treatment, lowering cholesterol, preventing stroke, regulating blood sugar, lowering fever, malaria, hepatitis, coughing, anti-inflammatory, antioxidants and others (Saraswati et al, 2019;Solikhah et al, 2021;Yeap et al, 2010). African leaves contain flavonoid compounds, alkaloids, tannins, saponins, terpenoids, and lactones sesquiterpenes ( Saraswati et al, 2019;Solikhah et al, 2021).…”