UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN  KEREHAU (Callicarpa longifolia Lam) TERHADAP Escherichia coli dan Staphylococcus aureus

Kusumawati, Eko; Apriliana, Anita; Khatimah, Khusnul

doi:10.51352/jim.v2i2.63

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…Menurut Chavan and Rhiley (2007) bakteriosin akan menghambat pertumbuhan bakteri yang reseptor permukaan selnya dikenali oleh senyawa tersebut. Reseptor utama tempat senyawa bakteriosin berikatan dengan bakteri yaitu lipid karena lipid bersifat nonpolar sehingga memudahkan masuknya senyawa bakteriosin yang bersifat nonpolar ke dalam sel bakteri (Kusumawati, 2016). Lipid merupakan penyusun dinding sel bakteri gram negatif paling banyak yaitu sebesar 11-12% dibandingkan kandungan peptidoglikan (Pelczar and Chan, 2005).…”

Section: Pembahasanunclassified

Aktivitas Antibakteri Metabolit Ekstraseluler Bacillus subtilis terhadap Shigella dysenteriae secara in vitro

Ambarwati

Ibrahim

2021

LenteraBio

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Shigella dysenteriae merupakan bakteri patogen penyebab shigellosis atau disentri basiler pada manusia dengan tingkat prevalensi yang tinggi. Shigella dysenteriae mengalami peningkatan resistensi dari tahun ke tahun sehingga diperlukan senyawa antibakteri baru yang diperoleh dari mikroorganisme, salah satunya yaitu bakteri Bacillus subtilis. Penelitian ini bertujuan untuk mendeskripsikan keefektifan filtrat metabolit ekstraseluler B. subtilis dan konsentrasi filtrat metabolit ekstraseluler yang optimal dari B. subtilis dalam menghambat pertumbuhan S. dysenteriae secara in vitro. Uji antibakteri filtrat metabolit ekstraseluler B. subtilis terhadap S. dysenteriae menerapkan metode difusi sumuran dengan perlakuan konsentrasi filtrat B. subtilis yang terdiri atas konsentrasi 100%, 90%, 80%, dan 70%, kontrol positif (Ampicilin 0,02%), dan kontrol negatif (Nutrient Broth) dengan 4 pengulangan. Filtrat metabolit ekstraseluler B. subtilis dipanen dengan sentrifugasi dan difiltrasi dengan mikrofilter 0,22µm. Analisis data diameter daerah penghambatan menggunakan Uji Kruskall Wallis dengan post hoc Bonferroni. Berdasarkan uji Kruskall-Wallis (0,01 < 0,05) menunjukkan bahwa konsentrasi filtrat metabolit ekstraseluler B. subtilis efektif dalam menghambat pertumbuhan S. dysenteriae. Semakin tinggi konsentrasi filtrat B. subtilis maka semakin tinggi daya hambat pertumbuhan terhadap S. dysenteriae. Konsentrasi filtrat metabolit ekstraseluler B. subtilis yang optimal dalam menghambat pertumbuhan S. dysenteriae berdasarkan post hoc Bonferroni dan tidak berbeda nyata jika dibandingkan dengan kontrol positif yaitu yaitu konsentrasi 80% (4,25 ± 0,96 mm), 90% (5,00 ± 1,23 mm), dan 100% (9,88 ± 3,45 mm). Konsentrasi filtrat metabolit ekstraseluler B. subtilis efektif dalam menghambat pertumbuhan S. dysenteriae secara in vitro sehingga berpotensi digunakan sebagai obat untuk mengatasi penyakit disentri basiler.

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Section: Pembahasanunclassified

Aktivitas Antibakteri Metabolit Ekstraseluler Bacillus subtilis terhadap Shigella dysenteriae secara in vitro

Ambarwati

Ibrahim

2021

LenteraBio

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“…Percobaan ini dilakukan secara triplo (3 cawan petri sekaligus). Dilakukan juga dengan cara yang sama terhadap bakteri Staphylococcus aureus (Kusumawati, 2016…”

Section: Uji Aktivitas Antibakteriunclassified

“…Berdasarkan Farmakope Indonesia (1995), daerah hambat efektif apabila menghasilkan batas daerah hambatan dengan diameter lebih kurang 14 mm sampai 16 mm. Kusumawati (2016), menjelaskan bahwa kategori zona hambat terbagi atas, kategori lemah yaitu kurang dari 5 mm, kategori sedang yaitu 5-10 mm, kategori kuat yaitu zona hambat 10-20 mm dan sangat kuat yaitu lebih dari 20 mm. Simplisia dan ekstrak etanol daun senduduk (Melastoma malabathricum L.) menunjukkan adanya senyawa flavonoid, saponin, tanin dan steroid/triterpenoid.…”

Section: Hasil Uji Skrining Fitokimia Ekstrak Daun Sendudukunclassified

Antibacterial Activity Test of the Ethanol in Leaves Extract of Senduduk (Melastoma malabathricum L.) Against Escherichia coli and Staphylococcus aureus

Sapitri

Lara

Sitorus

2020

JPBN

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Senduduk (Melastoma malabathricum L.) is one of the wild plants used as traditional medicine. Senduduk leaves are used as a medicine for sprue, boils, diarrhea and smallpox. The purpose of this study was to determine the antibacterial activity of the ethanol extract of senduduk leaves of the against Escherichia coli and Staphylococcus aureus. Senduduk leaves extracted by maceration using 96% ethanol solvent. Antibacterial activity testing is carried out using the diffusion method using paper discs.This experiment was repeated three times using concentrations of 20%, 40%, 60% and 80%.Ethanol extract of the senduduk leaves contains flavonoids, saponins, tannins and steroids/triterpenoids. The test results showed that the ethanol extract of the leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus which is characterized by the inhibition zone formed around the disc paper. In Escherichia coli bacteria the smallest inhibition zone at a concentration of 20% with a diameter of inhibition zone 11 mm and the largest diameter of the inhibitory zone at a concentration of 80% with a diameter of inhibition zone 19 mm. whereas for the smallest inhibitory zone Staphylococcus aureus bacteria at a concentration of 20% with an inhibition zone diameter of 12.6 mm and the largest inhibitory zone dameter at a concentration of 80% with a diameter of the inhibitory zone 21,3 mm.The results of the study concluded that the ethanol extract of Senduduk leaves has antibacterial activity against Escherichia coli and Staphylococcus aureus.

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“…Based on research performed by Eko Kusumawati et al, it is proven that the ethanol extract of sangkareho leaves has inhibitory power against the growth of Escherichia coli with the concentration of 25% and inhibit the growth of Staphylococcus aureus with the concentration of 10%. 8 Based on that, the aim of this study was to develop the potential of plant-based medicine, which is traditional medicines that functioned as antibacterial and effectiveness of ethanol extract of sangkareho (Callicarpa longifolia Lam.) leaves using Ultrasound-Assisted Extraction (UAE) extraction method for inhibiting the growth of S.typhi and S.epidermidis that haven't been researched before.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of extract sangkareho leaves (Callicarpa longifolia LAM.) on Salmonella typhi and Staphylococcus epidermidis

Permatasari,

Munthe,

Singa

et al. 2022

JKKI

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Background: The most commonly found disease-causing microorganisms are the Salmonella typhi and Staphylococcus epidermidis bacteria. S.typhi is a gram-negative bacterium that causes typhoid fever, and S.epidermidis is a gram-positive bacterium that attacks mucous membranes and human skin. Sangkareho (Callicarpa longifolia Lam.) is one of the indigenous herbal plants of Central Kalimantan, which is empirically used as a wound medicine, diarrhea, diabetic, anti-inflammatory, and can be an antibacterial agent.Objective: This study used six groups are concentrations of 10%, 25%, 50%, and 100%, Chloramphenicol as a positive control, and dimethyl sulfoxide (DMSO) as a negative control. Methods: The method used for this test is the well diffusion method. In Nutrient Agar (NA) media, holes were made to insert 40 μl of each extract concentration, positive and negative control with four repetitions, then incubated for 18-24 hours at 37ºC. Data analysis using the One Way Anova test.Results: The research findings showed that ethanol extract of sangkareho leaves with all concentrations had an inhibitory effect against both bacteria tested.Conclusion: The ethanol extract of sangkareho leaves at the concentration of 10% was found to be the optimum and at the concentration of 100% was the maximum effectiveness for inhibiting S. typhi and S. epidermidis.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEREHAU (Callicarpa longifolia Lam) TERHADAP Escherichia coli dan Staphylococcus aureus

Cited by 3 publications

References 3 publications

Aktivitas Antibakteri Metabolit Ekstraseluler Bacillus subtilis terhadap Shigella dysenteriae secara in vitro

Aktivitas Antibakteri Metabolit Ekstraseluler Bacillus subtilis terhadap Shigella dysenteriae secara in vitro

Antibacterial Activity Test of the Ethanol in Leaves Extract of Senduduk (Melastoma malabathricum L.) Against Escherichia coli and Staphylococcus aureus

Antibacterial activity of extract sangkareho leaves (Callicarpa longifolia LAM.) on Salmonella typhi and Staphylococcus epidermidis

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