UHPLC-Q-TOF/MS-Based Metabolomics Approach Reveals the Antifungal Potential of Pinocembroside against Citrus Green Mold Phytopathogen

Chen, Chuying; Cai, Nan; Chen, Jinyin; Wan, Chunpeng

doi:10.3390/plants9010017

Cited by 29 publications

(21 citation statements)

References 44 publications

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“…Three phenolics, namely, luteolin, apigenin, and umbelliferone, were putatively identified by comparison of extracted positive or negative ions with literature data, for which those were the main constituents previously reported [2,3]. Five flavonoid constituents (13)(14)(15)(16)(17) were all detected in EAE, AE, and ME of HFF. Furthermore, pinocembrin-7-O-β-D-glucoside (13), the main antifungal flavonoid, was also detected in CE of HFF.…”

Section: Hplc-qtof-ms Analysis Of Hff Extractsmentioning

confidence: 85%

“…Recently, three new monosubstituted benzene derivatives elucidated as (2R) methyl 2-O-β-d-glucopyranosyl-2-phenyl acetate, (2S) 2-O-benzoyl-butanedioic acid-4-methyl ester, and 4-O-benzoyl-quinic acid were initially isolated from the ethanolic extracts of HFF by our group members [8], and one new compound depicted a potent antifungal effect against Penicillium italicum isolated from citrus fruits. Continuing this research, our group members further isolated another three glycosides of flavonone, and pinocembrin-7-O-β-d-glucoside (PCBG) was said to be a major flavonoid in HFF and showed a prominent in vitro antifungal activity against P. italicum and P. digitatum [11,13]. The antifungal mechanism revealed by metabolomics showed that amino acids, lipids, tricarboxylic acid cycle, and ribonucleic acids all participated in the antifungal process [13].…”

Section: Introductionmentioning

confidence: 97%

“…Continuing this research, our group members further isolated another three glycosides of flavonone, and pinocembrin-7-O-β-d-glucoside (PCBG) was said to be a major flavonoid in HFF and showed a prominent in vitro antifungal activity against P. italicum and P. digitatum [11,13]. The antifungal mechanism revealed by metabolomics showed that amino acids, lipids, tricarboxylic acid cycle, and ribonucleic acids all participated in the antifungal process [13]. Furthermore, 5-O-[β-d -apiofuranosyl-(1→2)-β-d-glucopyranosyl]-bergaptol was the first ever reported furanocoumarin glycoside extracted from the roots of F. hirta Vahl.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

et al. 2020

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The medicinal and edible plant, Ficus hirta Vahl. (also called hairy fig), is used for the treatment of constipation, inflammation, postpartum hypogalactia, tumors, and cancer. There is an urgent need for scientific evaluation to verify the pharmacological properties of F. hirta. Therefore, in vitro assays evaluated the antioxidant and antifungal activities of various solvent extracts of hairy fig fruits (HFF). HFF extracts had abundant antioxidant components for a significant amount of total phenolic (TPC) and flavonoid contents (TFC) (TPC from 17.75 ± 0.52 to 85.25 ± 1.72 mg gallic acid/g dw and TFC from 15.80 ± 0.59 to 144.22 ± 8.46 mg rutin/g dw, respectively). The ethyl acetate extract (EAE) and acetone extract (AE) of HFF demonstrated potent antioxidant activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50 values of 2.52 and 2.02 mg/mL, respectively) and ABTS radicals (IC50 values of 3.06 and 9.26 mg/mL, respectively). Moreover, the AE with a high TFC showed a prominent in vitro and in vivo antifungal activity against Penicillium italicum, causing citrus blue mold. Eighteen metabolites were identified or putatively identified from six HFF extracts. Current findings indicated that HFF extracts had significant antioxidant and antifungal activities and could potentially be used as an alternative agent for the preservation of agricultural products.

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Section: Hplc-qtof-ms Analysis Of Hff Extractsmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

et al. 2020

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Section: Introductionmentioning

confidence: 99%

“…A previous study which bore out the antifungal activity of P7G on P. italicum in potato dextrose agar (PDA) media showed that P7G was successful at inhibiting both Penicillium spp. mycelial growth [23,28,29]. However, to date, there have been no reports on the antifungal activity and possible action mechanisms of P7G on postharvest pathogenic fungus in citrus fruit, especially those concerning P. italicum, which infects fresh citrus fruit.…”

Section: Introductionmentioning

confidence: 99%

Pinocembrin-7-Glucoside (P7G) Reduced Postharvest Blue Mold of Navel Orange by Suppressing Penicillium italicum Growth

Chen

Wan

2020

Microorganisms

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The current study aimed to examine the in vitro and in vivo antifungal potential of pinocembrin-7-glucoside (P7G). P7G is an antifungal flavanone glycoside isolated from Ficus hirta Vahl. fruit against Penicillium italicum, a causative pathogen of blue mold disease in citrus fruit, and this study elucidates its possible action mechanism. P7G had a prominent mycelial growth inhibitory activity against P. italicum, with an observed half maximal effective concentration, minimum inhibitory concentration and minimum fungicidal concentration of 0.08, 0.2, and 0.8 g/L, respectively. The data from the in vivo test show that P7G significantly reduced blue mold symptoms and disease development of P. italicum in artificially inoculated “Newhall” navel orange. Compared to the control, increases in the cell membrane permeability of P. italicum supernatant and decreases in the intracellular constituent (e.g., soluble protein, reducing sugar, and total lipid) contents of P. italicum mycelia were identified, supporting scanning electron microscopy and transmission electron microscopy observations. Furthermore, a marked decline in both chitin and glucanase contents of P. italicum mycelia treated with P7G was induced by increasing its related degrading enzyme activities, suggesting that the cell wall structure was destroyed. The current study indicated that P7G may be a novel alternative for reducing blue mold by suppressing mycelial growth of P. italicum via a cell membrane/wall-targeting mechanism.

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Control of postharvest diseases caused by Penicillium spp. with myrtle leaf phenolic extracts: in vitro and in vivo study on mandarin fruit during storage

et al. 2021

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BACKGROUND In the postharvest handling of horticultural commodities, plant extracts with fungicidal activity are a valid alternative to synthetic fungicides. The fungicidal activity of myrtle leaf extracts from eight cultivars was studied in vitro against Penicillium digitatum, Penicillium italicum, and Penicillium expansum and on artificially inoculated mandarins with green and blue molds during storage for 12 days at 20 °C and 90% RH. RESULTS Hydroxybenzoic acids, hydrolysable tannins, and flavonols were identified by high‐performance liquid chromatography (HPLC). Despite sharing the same phenolic profile, extracts of eight myrtle cultivars significantly differed in the concentration of phenolics. Hydrolysable tannins are the principal subclass representing nearly 44.9% of the total polyphenols, whereas myricitrin was the most abundant flavonol in all cultivars. Myrtle extracts strongly inhibited conidial germination of the pathogens tested, although the greatest efficacy was observed against P. digitatum. At a concentration of 20 g L−1, all the extracts completely inhibited fungi growth; only ‘Angela’, ‘Tonina’ and ‘Grazia’ extracts were effective at lower concentrations (15 g L−1). On inoculated fruit, myrtle extracts significantly controlled rot development. As a preventive treatment, ‘Ilaria’ and ‘Maria Rita’ extracts significantly reduced the rate of fruit with green mold decay lesions. When applied as a curative treatment, all the exacts decreased the incidence of decay. Against P. italicum, all the extracts applied as preventive treatments controlled decay effectively, while as curative treatment some of the extracts were not effective. All the extracts reduced the size of the infected areas. CONCLUSION The results propose myrtle extracts as a possible natural alternative to synthetic fungicides. © 2021 Society of Chemical Industry

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UHPLC-Q-TOF/MS-Based Metabolomics Approach Reveals the Antifungal Potential of Pinocembroside against Citrus Green Mold Phytopathogen

Cited by 29 publications

References 44 publications

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

Antioxidant, Antifungal Activities of Ethnobotanical Ficus hirta Vahl. and Analysis of Main Constituents by HPLC-MS

Pinocembrin-7-Glucoside (P7G) Reduced Postharvest Blue Mold of Navel Orange by Suppressing Penicillium italicum Growth

Control of postharvest diseases caused by Penicillium spp. with myrtle leaf phenolic extracts: in vitro and in vivo study on mandarin fruit during storage

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