UDP-glucose sterol ß-d-glucosyltransferase, a plasma membrane-bound enzyme of plants: Enzymatic properties and lipid dependence

Compartmentation and topology of glucosylceramide synthesis

“…These results are consistent with previous reports localizing sterol glucosyltransferase primarily to the plasma membrane [7,8], and are the first to localize GlcCer synthase to the plasma membrane in plant cells. However, the possibility that the two enzymes are associated with another compartment (e.g.…”

Compartmentation and topology of glucosylceramide synthesis

“…These results are consistent with previous reports localizing sterol glucosyltransferase primarily to the plasma membrane [7,8], and are the first to localize GlcCer synthase to the plasma membrane in plant cells. However, the possibility that the two enzymes are associated with another compartment (e.g.…”

Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots

“…We identified the fluorescent bodipy dye ER Tracker (587 excitation nm, 615 nm emission) as a vehicle to test whether fluorescence from UGT80B1:GFP pixels colocalized with pixels from the ER dye and results supported colocalization of the UGT80B1:GFP (Supplementary Figure S3) and the positive control ER reporter Q4:GFP line 34 . Using the same experimental rationale, we employed the membrane reporter FM4–64 (515 nm excitation, 640 nm emission maxima) to visualize colocalization at the PM after short term (5 min) exposure using PLASMAMEMBRANE INTRINSIC PROTEIN2 (PIP2):GFP as positive control, which also revealed satisfactory colocalization (Supplementary Figure S4) to support previous biochemical reports 31–33 .…”

“…As suggested from previous reports 31–33 , UDP-Glc:sterol glucosyltransferase was hypothesized to be localized in the ER (endomembrane) and possibly also in the plasma membrane (PM). To test this hypothesis, we visualized UGT80B1:GFP in the subcortical focal plane and could observe features that appeared reminiscent of the highly dynamic cisternae of the ER (Movie S3).…”

Application of wide selected‐ion monitoring data‐independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor

“…A role for CD2 in the regulation of developmental processes is further suggested by the identification of a sterol‐3‐beta‐glucosyltransferase among the proteins affected by the CD2 mutation. Sterol‐3‐beta‐glucosyltransferases are membrane‐bound proteins that catalyze the glycosylation of plant sterols , which are central to the control of cell division, elongation, polarity, and cellulose accumulation . In addition, two proteins found to accumulate more in cd2 than in M82, Solyc10g008140 (prohibitin 1‐like protein) and Solyc02g089070 (eukaryotic translation initiation factor 3 subunit M) are homologs of proteins that have been shown to have a role in plant development .…”

Compartmentation and topology of glucosylceramide synthesis

“…These results are consistent with previous reports localizing sterol glucosyltransferase primarily to the plasma membrane [7,8], and are the first to localize GlcCer synthase to the plasma membrane in plant cells. However, the possibility that the two enzymes are associated with another compartment (e.g.…”

Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots

“…We identified the fluorescent bodipy dye ER Tracker (587 excitation nm, 615 nm emission) as a vehicle to test whether fluorescence from UGT80B1:GFP pixels colocalized with pixels from the ER dye and results supported colocalization of the UGT80B1:GFP (Supplementary Figure S3) and the positive control ER reporter Q4:GFP line 34 . Using the same experimental rationale, we employed the membrane reporter FM4–64 (515 nm excitation, 640 nm emission maxima) to visualize colocalization at the PM after short term (5 min) exposure using PLASMAMEMBRANE INTRINSIC PROTEIN2 (PIP2):GFP as positive control, which also revealed satisfactory colocalization (Supplementary Figure S4) to support previous biochemical reports 31–33 .…”

“…As suggested from previous reports 31–33 , UDP-Glc:sterol glucosyltransferase was hypothesized to be localized in the ER (endomembrane) and possibly also in the plasma membrane (PM). To test this hypothesis, we visualized UGT80B1:GFP in the subcortical focal plane and could observe features that appeared reminiscent of the highly dynamic cisternae of the ER (Movie S3).…”

Application of wide selected‐ion monitoring data‐independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor

“…A role for CD2 in the regulation of developmental processes is further suggested by the identification of a sterol‐3‐beta‐glucosyltransferase among the proteins affected by the CD2 mutation. Sterol‐3‐beta‐glucosyltransferases are membrane‐bound proteins that catalyze the glycosylation of plant sterols , which are central to the control of cell division, elongation, polarity, and cellulose accumulation . In addition, two proteins found to accumulate more in cd2 than in M82, Solyc10g008140 (prohibitin 1‐like protein) and Solyc02g089070 (eukaryotic translation initiation factor 3 subunit M) are homologs of proteins that have been shown to have a role in plant development .…”

Compartmentation and topology of glucosylceramide synthesis

“…These results are consistent with previous reports localizing sterol glucosyltransferase primarily to the plasma membrane [7,8], and are the first to localize GlcCer synthase to the plasma membrane in plant cells. However, the possibility that the two enzymes are associated with another compartment (e.g.…”

Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots

“…We identified the fluorescent bodipy dye ER Tracker (587 excitation nm, 615 nm emission) as a vehicle to test whether fluorescence from UGT80B1:GFP pixels colocalized with pixels from the ER dye and results supported colocalization of the UGT80B1:GFP (Supplementary Figure S3) and the positive control ER reporter Q4:GFP line 34 . Using the same experimental rationale, we employed the membrane reporter FM4–64 (515 nm excitation, 640 nm emission maxima) to visualize colocalization at the PM after short term (5 min) exposure using PLASMAMEMBRANE INTRINSIC PROTEIN2 (PIP2):GFP as positive control, which also revealed satisfactory colocalization (Supplementary Figure S4) to support previous biochemical reports 31–33 .…”

“…As suggested from previous reports 31–33 , UDP-Glc:sterol glucosyltransferase was hypothesized to be localized in the ER (endomembrane) and possibly also in the plasma membrane (PM). To test this hypothesis, we visualized UGT80B1:GFP in the subcortical focal plane and could observe features that appeared reminiscent of the highly dynamic cisternae of the ER (Movie S3).…”

Application of wide selected‐ion monitoring data‐independent acquisition to identify tomato fruit proteins regulated by the CUTIN DEFICIENT2 transcription factor

“…A role for CD2 in the regulation of developmental processes is further suggested by the identification of a sterol‐3‐beta‐glucosyltransferase among the proteins affected by the CD2 mutation. Sterol‐3‐beta‐glucosyltransferases are membrane‐bound proteins that catalyze the glycosylation of plant sterols , which are central to the control of cell division, elongation, polarity, and cellulose accumulation . In addition, two proteins found to accumulate more in cd2 than in M82, Solyc10g008140 (prohibitin 1‐like protein) and Solyc02g089070 (eukaryotic translation initiation factor 3 subunit M) are homologs of proteins that have been shown to have a role in plant development .…”