Ubiquitous presence of gluconeogenic regulatory enzyme, fructose-1,6-bisphosphatase, within layers of rat retina

Mamczur, Piotr; Mazurek, Jakub; Rakus, Dariusz

doi:10.1007/s00441-010-1008-2

Cited by 17 publications

(16 citation statements)

References 34 publications

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“…In contrast to dividing cells, FBPase is absent in nuclei of terminally differentiated cells (Gizak et al 2006; Mamczur et al 2010). These findings point to FBPase as a protein potentially involved in cell cycle regulation and/or progression.…”

Section: Discussionmentioning

confidence: 99%

Cell cycle-dependent expression and subcellular localization of fructose 1,6-bisphosphatase

Mamczur

Sok

Rzechonek³

et al. 2011

Histochem Cell Biol

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Recently a gluconeogenic enzyme was discovered—fructose 1,6-bisphosphatase (FBPase)—that localizes in the nucleus of a proliferating cell, but its physiological role in this compartment remains unclear. Here, we demonstrate the link between nuclear localization of FBPase and the cell cycle progression. Results of our studies indicate that in human and mouse squamous cell lung cancer, as well as in the HL-1 cardiomyocytes, FBPase nuclear localization correlates with nuclear localization of S and G2 phase cyclins. Additionally, activity and expression of the enzyme depends on cell cycle stages. Identification of FBPase interacting partners with mass spectrometry reveals a set of nuclear proteins involved in cell cycle regulation, mRNA processing and in stabilization of genomic DNA structure. To our knowledge, this is the first experimental evidence that muscle FBPase is involved in cell cycle events.Electronic supplementary materialThe online version of this article (doi:10.1007/s00418-011-0884-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Cell cycle-dependent expression and subcellular localization of fructose 1,6-bisphosphatase

Mamczur

Sok

Rzechonek³

et al. 2011

Histochem Cell Biol

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“…This enzyme catalyzes the cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. In many cells, the presence of aldolase in nucleus was observed [51][52][53], and the studies indicated the ability of aldolase to interact with some DNA sequences. This data revealed that such activity may require a minimal DNA sequence and/or conformation.…”

Section: Diphosphate Aldolase (Aldo)mentioning

confidence: 95%

“…Their studies revealed that blocking of the cell cycle progression through the S phase and the inhibition of transcription promoted the removal of ALDA from the nuclei. It was found previously that aldolase A was localized in nuclei of proliferating cells within neonatal retina [52]. Thus, it might be hypothesized that nuclear localization of ALDA is not restricted to cancer cells but it is a common feature of proliferating cells.…”

Section: Diphosphate Aldolase (Aldo)mentioning

confidence: 96%

Enzymes of the central carbon metabolism: Are they linkers between transcription, DNA replication, and carcinogenesis?

et al. 2015

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“…FBP1 localizes to the nuclei of proliferating cells (47,48) and is recognized to be important for the regulation of gluconeogenesis (49,50). The FBP1 gene is also expressed in certain cancer cells; however, its expression levels are reduced by comparison with non-malignant tissues (27,29).…”

Section: Discussionmentioning

confidence: 99%

FBP1 expression is associated with basal-like breast carcinoma

Shi

Zhao

et al. 2017

Oncology Letters

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The present study aimed to investigate the value of liver fructose 1,6-bisphophatase (FBP1) and hypoxia-inducible factor-1α (HIF-1α) in the molecular subtyping of breast carcinoma. Tissue obtained from 60 surgical specimens from patients with breast carcinoma underwent immunohistochemical staining for cytokeratin 5/6, HIF-1α and FBP1. The variation in the expression levels of these markers and clinicopathological factors were compared between molecular subtypes. In addition, disease-free survival was compared between basal-like and luminal breast carcinoma, according to differing expression levels of HIF-1α and FBP1. The results revealed that HIF-1α expression was detectable in 20/60 (33.3%) of the breast carcinoma cases, and was positively associated with lymph node metastasis (P=0.007). HIF-1α-positive patients exhibited a shorter disease-free survival, compared with HIF-1α-negative patients with invasive breast cancer. The expression levels of FBP1 were positive in 33/60 tumor tissues (55%; P<0.001), and FBP1 expression was associated with nuclear grade (P=0.017) and tumor stage (P=0.012). In breast carcinoma, HIF-1α expression levels were significantly negatively correlated with FBP1 levels (r=−0.711; P<0.001). Cox regression analysis identified FBP1 and tumor size as independent prognostic factors. Therefore, the present study demonstrated that patients with basal-like breast carcinoma exhibited lower levels of FBP1 expression in tumor tissues, compared with patients with luminal type breast cancer, and that low or absent expression levels of FBP1 may be associated with reduced disease-free survival.

show abstract

Ubiquitous presence of gluconeogenic regulatory enzyme, fructose-1,6-bisphosphatase, within layers of rat retina

Cited by 17 publications

References 34 publications

Cell cycle-dependent expression and subcellular localization of fructose 1,6-bisphosphatase

Cell cycle-dependent expression and subcellular localization of fructose 1,6-bisphosphatase

Enzymes of the central carbon metabolism: Are they linkers between transcription, DNA replication, and carcinogenesis?

FBP1 expression is associated with basal-like breast carcinoma

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