Tyrosine hydroxylase-immunoreactivity and its relations with gonadotropin-releasing hormone and neuropeptide Y in the preoptic area of the guinea pig

Bogus‐Nowakowska, Krystyna; Równiak, Maciej; Hermanowicz-Sobieraj, Beata; Wasilewska, B; Najdzion, Janusz; Robak, Anna

doi:10.1016/j.jchemneu.2016.09.008

Cited by 3 publications

(4 citation statements)

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“…POA neurons, besides GABA, estrogen and androgen receptors, express many neurotransmitters and neuropeptides (Bogus‐Nowakowska et al ., , ; Hermanowicz et al ., ; Bogus‐Nowakowska et al ., ) but there is a scarcity of data on coexpression profile of CaBPs‐containing neurons in this brain region. CaBPs are present in different neuronal populations, such as cholinergic, GABAergic, glutamatergic, and nitrinergic (Baimbridge et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…It is important for endocrine activity and crucial for the expression of sexual behavior (Dominguez and Hull, ) as it receives inputs from sensory modalities (Simerly and Swanson, ) and gives projections to other structures that are critical for the initiation and patterning of copulation (Simerly and Swanson, ). The PPN and MPN of the guinea pig provide the majority of gonadoliberin (GnRH) containing neurons participating in the LH surge (Bogus‐Nowakowska et al ., ). The LPA participates in circadian control of sleep–wake cycles (Deurveilher et al ., ) and it appears to be a critical component of sleep circuitry across multiple species (Gaus et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Bogus‐Nowakowska

2019

Developmental Neurobiology

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This study characterizes for the first time the distribution and coexistence patterns of calbindin (CB), calretinin (CR), and parvalbumin (PV) in the female and male guinea pig preoptic area (POA) during brain development, using immunohistochemistry and quantitative real‐time PCR techniques. The results show that the prenatal development of the guinea pig POA takes place in elevated levels of CB and CR immunoreactivity with the peak at embryonic day 50 (E50) and generally in newborns both these proteins reach an adult‐like pattern of immunoreactivity, contrary to PV which appears later, peaks at postnatal day (PND) 10 (P10), and stabilizes at P20. CB and CR have also overlapping distributions which differed from that of PV, and much higher expressions at mRNA and protein levels. However, CB‐positive (+), CR+ and PV+ neurons create in the guinea pig POA separate populations as CB and CR coexisted only in a small number of neurons and CB+ cells never coexpressed PV. Moreover, the density of CB+ neurons, contrary to CR+ and PV+ cells, is sexually dimorphic favoring males at all the examined stages. In conclusion, elevated levels of CR and CB at the time of intense cell migration, differentiation, myelination, and synaptogenesis in the guinea pig brain suggest that these proteins may be engaged in similar processes in the POA, while late onset of PV may be rather linked with POA maturation. As the population of CB+ cells in the POA is very large, its dimorphic development may have huge impact on the sexual differentiation of this brain region.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Bogus‐Nowakowska

2019

Developmental Neurobiology

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“…Moreover, these antibodies have been positively evaluated using Western blotting, immunohistochemistry in knockout mouse brain sections, and immunoprecipitation, justifying their specificity to their targets ( 63 , 64 , 66 ). The mouse antibody toward TH (MAB 318) is commonly used in brain studies on dopaminergic neurotransmission ( 67 , 68 ). The specificity of secondary antibodies was examined by the omission of the primary antibody and its replacement by nonimmune sera or PBS.…”

Section: Methodsmentioning

confidence: 99%

Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight

Bogdańska-Chomczyk,

Równiak,

Huang

et al. 2024

Front. Psychiatry

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BackgroundAttention deficit hyperactivity disorder (ADHD) is characterized by impairments in developmental–behavioral inhibition, resulting in impulsivity and hyperactivity. Recent research has underscored cortical inhibition deficiencies in ADHD via the gamma-aminobutyric acid (GABA)ergic system, which is crucial for maintaining excitatory–inhibitory balance in the brain. This study explored postnatal changes in parvalbumin (PV) immunoreactivity, indicating GABAergic interneuron types, in the prefrontal (PFC) and motor (MC) cortices of spontaneously hypertensive rats (SHRs), an ADHD animal model.MethodsExamining PV- positive (PV+) cells associated with dopamine D2 receptors (D2) and the impact of dopamine on GABA synthesis, we also investigated changes in the immunoreactivity of D2 and tyrosine hydroxylase (TH). Brain sections from 4- to 10-week-old SHRs and Wistar Kyoto rats (WKYs) were immunohistochemically analyzed, comparing PV+, D2+ cells, and TH+ fiber densities across age-matched SHRs and WKYs in specific PFC/MC regions.ResultsThe results revealed significantly reduced PV+ cell density in SHRs: prelimbic (~20% less), anterior cingulate (~15% less), primary (~15% less), and secondary motor (~17% less) cortices. PV+ deficits coincided with the upregulation of D2 in prepubertal SHRs and the downregulation of TH predominantly in pubertal/postpubertal SHRs.ConclusionReduced PV+ cells in various PFC regions could contribute to inattention/behavioral alterations in ADHD, while MC deficits could manifest as motor hyperactivity. D2 upregulation and TH deficits may impact GABA synthesis, exacerbating behavioral deficits in ADHD. These findings not only shed new light on ADHD pathophysiology but also pave the way for future research endeavors.

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“…As it receives inputs from diverse brain regions that convey sensory and autonomic signals relevant to reproduction [ 27 ], the region is crucial for integrating hormonal and environmental signals and communicating them to gonadoliberin (GnRH) neurons. In the POA, especially the PPN and MPN provide the majority of GnRH-containing neurons [ 28 ], which induce the pituitary to release follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the hypothalamic pituitary–gonadal axis [ 29 ]. GABA and glutamate are predominant transmitters of the hypothalamus, and GABAergic and glutamatergic transmissions are important for both the negative and positive feedback mechanism on the GnRH release [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

GABAergic and Glutamatergic Phenotypes of Neurons Expressing Calcium-Binding Proteins in the Preoptic Area of the Guinea Pig

Bogus‐Nowakowska

Robak

Kalinowski

et al. 2022

IJMS

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The mammalian preoptic area (POA) has large populations of calbindin (CB), calretinin (CR) and parvalbumin (PV) neurons, but phenotypes of these cells are unknown. Therefore, the question is whether neurons expressing CB, CR, and/or PV are GABAergic or glutamatergic. Double-immunofluorescence staining followed by epifluorescence and confocal microscopy was used to determine the coexpression patterns of CB, CR and PV expressing neurons with vesicular GABA transporters (VGAT) as specific markers of GABAergic neurons and vesicular glutamate transporters (VGLUT 2) as specific markers of glutamatergic neurons. The guinea pig was adopted as, like humans, it has a reproductive cycle with a true luteal phase and a long gestation period. The results demonstrated that in the guinea pig POA of both sexes, ~80% of CB+ and ~90% of CR+ neurons coexpress VGAT; however, one-fifth of CB+ neurons and one-third of CR+ cells coexpress VGLUT. About two-thirds of PV+ neurons express VGAT, and similar proportion of them coexpress VGLUT. Thus, many CB+, CR+ and PV+ neurons may be exclusively GABAergic (VGAT-expressing cells) or glutamatergic (VGLUT-expressing cells); however, at least a small fraction of CR+ cells and at least one-third of PV+ cells are likely neurons with a dual GABA/glutamate phenotype that may coexpress both transporters.

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Tyrosine hydroxylase-immunoreactivity and its relations with gonadotropin-releasing hormone and neuropeptide Y in the preoptic area of the guinea pig

Cited by 3 publications

References 50 publications

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Ontogeny of Neurons Containing Calcium‐Binding Proteins in the Preoptic Area of the Guinea Pig: Sexually Dimorphic Development of Calbindin

Parvalbumin interneuron deficiency in the prefrontal and motor cortices of spontaneously hypertensive rats: an attention-deficit hyperactivity disorder animal model insight

GABAergic and Glutamatergic Phenotypes of Neurons Expressing Calcium-Binding Proteins in the Preoptic Area of the Guinea Pig

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