Typing of mutans streptococci by arbitrarily primed polymerase chain reaction

Saarela, Maria; Hannula, J.; Mättö, Jaana; Asikainen, Sirkka; Alaluusua, Satu

doi:10.1016/s0003-9969(96)00049-0

Cited by 60 publications

(64 citation statements)

References 20 publications

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“…It has been shown that the polymorphism in length of the amplified sequences (amplicons) of RAPD fingerprints obtained by AP-PCR can be used to compare bacterial strains [22][23][24], including the groups of mutans streptococci [28,29] and mitis streptococci [30]. In the present study, this application of RAPD analysis to genotyping of oral streptococci confirmed that the RAPD assay can distinguish the species S. mutans and S. sobrinus from each other.…”

Section: Discussionsupporting

confidence: 77%

“…They include DNA-DNA hybridisation [1,12,14], restriction endonuclease analysis (REA) [16][17][18][19], multilocus enzyme electrophoresis (MLEE) [20], ribotyping, PCR and colony hybridisation with DNA probes [21]. A PCR-based procedure of DNA fingerprinting, called arbitrarily primed PCR (AP-PCR) [22] or randomly amplified polymorphic DNA (RAPD) analysis [23], has been applied to strain identification and typing of oral bacteria [24][25][26][27], oral streptococci [28][29][30] and non-oral streptococci [31][32][33][34]. The present study used RAPD analysis for typing S. mutans and S. sobrinus strains and to explore the potential of this typing method to distinguish these from the other taxa that comprise the group of mutans streptococci.…”

Section: Introductionmentioning

confidence: 99%

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Identification of mutans and other oral streptococci by random amplified polymorphic DNA analysis

Truong¹,

Ménard

Mouton³

et al. 2000

Journal of Medical Microbiology

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The identification and classification of the non-haemolytic or viridans group of streptococci have long been recognised as difficult and unsatisfactory. Phenotypic and genotypic heterogeneity have resulted in ambiguous speciation, particularly with mutans streptococci and other oral streptococci. This study was done to determine whether random amplified polymorphic DNA (RAPD) analysis is useful to identify and even classify oral and other streptococci. DNA was prepared and purified from 25 strains of mutans streptococci including 11 reference strains of Streptococcus mutans, seven of S. sobrinus, three of S. rattus and one each of the four other species of the mutans group, together with 20 other reference species, mostly streptococci, and from 49 fresh isolates of mutans streptococci and of S. mutans from human saliva and dental plaque. DNA amplification was primed with each of three arbitrarily selected primers nine or 10 nucleotides in length. The amplified DNA fragments (amplicons) obtained were compared by agarose gel electrophoresis. Species-and strain-specific RAPD fingerprints were obtained not only from pure genomic DNA, but also from the supernates of crude cellular or colony extracts. Pending the analysis of numerous other strains, the data suggest that RAPD may be of value: (i) to distinguish the species S. mutans and S. sobrinus from each other and potentially from other species of oral streptococci, (ii) to differentiate and possibly classify oral streptococci and (iii) as a valuable tool in mutans streptococci epidemiology and transmission studies, by virtue of its rapidity, efficiency and reproducibility in generating genetic fingerprints of streptococcal isolates.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Identification of mutans and other oral streptococci by random amplified polymorphic DNA analysis

Truong¹,

Ménard

Mouton³

et al. 2000

Journal of Medical Microbiology

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“…Usually the mother is regarded as the main source of the organisms found in children, based on chromosomal DNA-fingerprinting techniques used for the study of intrafamily clonal distribution (Alaluusua et al, 1994;Li & Caufield, 1995;Saarela et al, 1996). Straetemans et al (1998) suggested that a delayed acquisition of mutans streptococci may reduce the incidence of caries in both primary and permanent dentition later on.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children

Okada

Soda

Hayashi

et al. 2005

Journal of Medical Microbiology

109

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Streptococcus mutans and Streptococcus sobrinus are known to be associated with the development of dental caries. In this study these bacteria were detected in pre-school children (each with primary dentition, age range 3-5 years, n ¼ 60) using a PCR method, and then their presence was compared with the incidence of dental caries over a 1-year period. Plaque samples were collected from all erupted tooth sites using a sterile toothbrush. Dental examinations at the beginning of the study (baseline) and after 1 year were also performed to determine decayed, missing, filled teeth (dmft) scores using WHO caries diagnostic criteria. The prevalences of S. mutans and S. sobrinus across all the subjects were 61 . 7 % and 56 . 6 %, respectively; 13 subjects (21 . 7 %) were positive for S. mutans alone, 10 (16 . 6 %) were positive for S. sobrinus alone and 24 (40 . 0 %) were positive for both S. mutans and S. sobrinus, whereas 13 (21 . 7 %) were negative for both S. mutans and S. sobrinus. dmft scores of subjects positive for both S. mutans and S. sobrinus at baseline and after 1 year were significantly higher than of those positive for S. mutans alone at the same stages (P , 0 . 01 and P , 0 . 001, respectively). The caries incremental increase was also significantly greater in those with both bacteria detected (P , 0 . 05). Our results indicate that pre-school children harbouring both S. mutans and S. sobrinus have a significantly higher incidence of dental caries than those with S. mutans alone. INTRODUCTIONMutans streptococci (Streptococcus mutans and Streptococcus sobrinus) are considered to be major dental caries aetiologic agents. They are the most common pathogens isolated from human dental plaque and their prevalence has been reported in epidemiological studies (Hamada & Slade, 1980;Whiley & Beighton, 1998). S. mutans has been shown to be more prevalent than S. sobrinus in dental plaque samples (Loesche, 1986; Carlsson et al., 1987), while several epidemiological studies have shown that the prevalence of S. sobrinus is more closely associated with high caries activity (Fujiwara et al., 1991;Hirose et al., 1993).In several epidemiological studies, identification of S. mutans and S. sobrinus on selective media such as mitis-salivarius (MS) or MS-bacitracin (MSB) agar has been performed using colonial morphology (Wade et al., 1986;Svanberg & Krasse, 1990). However, accurate differentiation between S. mutans and S. sobrinus is not easy, and is also timeconsuming and laborious (de Soet et al., 1990). Further, it has been reported that S. sobrinus from dental plaque samples is especially difficult to culture directly on MSB selective medium (Jordan 1986;de Soet et al., 1990). Thus, it is of great importance to distinguish the presence of these two species separately in children for accurate prediction and effective prevention of dental caries.Thus far, several methods used for detecting and identifying mutans streptococci have been reported, including direct microscopy, cultivation, enzyme tests, mAbs, ELISAs and specie...

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“…The dental biofilm consistes of a complex bacterial community and the ability of specific strains of Streptococcus mutans (S. mutans) to compete with other strains may be essential for colonization (Loesche 1986;Becker et al 2002). S. mutans show high phenotypic and genotypic heterogeneities (Mattos-Graner et al 2000, Mattos-Graner et al 2001Saarela et al 1996) and some genotypes may be able to colonize the host and induce dental caries better than other genotypes. This hypothesis has been reinforced by the findings of significant variations in caries induction potential in the animal models when different S. mutans strains were compared (Köhler and Krasse 1990).…”

Section: Introductionmentioning

confidence: 99%

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals

Braga

Piovesan

Valarini

et al. 2013

Braz. arch. biol. technol.

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Typing of mutans streptococci by arbitrarily primed polymerase chain reaction

Cited by 60 publications

References 20 publications

Identification of mutans and other oral streptococci by random amplified polymorphic DNA analysis

Identification of mutans and other oral streptococci by random amplified polymorphic DNA analysis

Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children

Genotypic diversity and virulence factors of Streptococcus mutans in caries-free and caries-active individuals

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