Typing of canine parvovirus strains circulating in Brazil between 2008 and 2010

Pinto, Luciane Dubina; Streck, André Felipe; Gonçalves, Karla; Souza, Carine K.; Corbellini, Ângela Oliveira; Corbellini, Luís Gustavo; Canal, Cláudio Wageck

doi:10.1016/j.virusres.2012.01.001

Cited by 53 publications

(51 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In accordance with Pinto et al (2012), the amino acid substitution Thr440Ala was not observed in any CPV-2c sequences, as previously described in CPV-2a and CPV-2b strains from Italy (Battilani et al 2002), India (Chinchkar et al 2006), Korea (Kang et al 2008), and in CPV-2c strains from Argentina (Calderón et al 2011) and Italy (Decaro et al 2009). The 440 position is located at an important VP2 domain, undergoing positive selection and envolved independently in different populations ).…”

Section: Discussionsupporting

confidence: 87%

“…Pinto et al (2012) demonstrated that CPV-2c has been the main subtype detected in the canine population of some regions of Brazil, as previously found in the Uruguay (Pérez et al 2007), Italy ), Germany (Decaro et al 2011a), and Argentina (Calderón et al 2011). Therefore, it is important to identify emerging CPV variants and developing preventive measures to control the spread of CPV-2 variants.…”

Section: Introductionmentioning

confidence: 52%

See 1 more Smart Citation

A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

et al. 2013

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 52%

A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

et al. 2013

View full text Add to dashboard Cite

“…In our study, only one dog was infected with the genotype CPV-2a and another one with CPV-2c. Pinto et al (2010), a group of researchers from Rio Grande do Sul, Brazil, studied samples from six different Brazilian states and found CPV-2c to be the predominant subtype. The predominance of CPV-2c was reported by Calderón et al (2011) in Argentina, and the close proximity of Argentina to the Rio Grande do Sul suggests that regional viral circulation could be responsible for the detection of the same subtypes.…”

Section: Discussionmentioning

confidence: 99%

“…The authors concluded that there was no interference in the ability of commercial vaccines to protect puppies against circulating canine parvovirus, but they did suggest the need to continue monitoring for new variants. Pinto et al (2010) reported that for 42 canine parvovirus-positive samples, 78.6% (33/42) of the samples were type 2c, 19% (8/42) were type 2b, and 2.4% (1/42) were type 2a.…”

Section: Introductionmentioning

confidence: 99%

Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

et al. 2016

View full text Add to dashboard Cite

Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70's new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified , CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central--West region of São Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination test and those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in São Paulo region.INDEX TERMS: Canine parvovirus, PCR, viral type, dogs.

show abstract

“…During the last ten years, a novel CPV mutant, CPV-2c, has widely distributed and co-exists with other CPV types in Europe , North (Hong et al 2007) and South (Perez et al 2007) America countries. The identification of the subtypes of CPV-2 that are currently circulating in the canine population is essential for the understanding of viral evolution and the development of measures to control its spread (Pinto et al 2012). All the three antigenic variants differ from the original type CPV-2 for a few amino acids in the VP2 protein, whereas genetic differences among the variants are determined only by residue 426, with types 2a, 2b, and 2c displaying Asn, Asp, and Glu, respectively (Martella et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples

Zienius¹,

Lelešius²,

Kavaliauskis³

et al. 2016

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The aim of the present study was to detect canine parvovirus (CPV) from faecal samples of clinically ill domestic dogs by polymerase chain reaction (PCR) followed by VP2 gene partial sequencing and molecular characterization of circulating strains in Lithuania.Eleven clinically and antigen-tested positive dog faecal samples, collected during the period of 2014-2015, were investigated by using PCR. The phylogenetic investigations indicated that the Lithuanian CPV VP2 partial sequences (3025-3706 cds) were closely related and showed 99.0-99.9% identity. All Lithuanian sequences were associated with one phylogroup, but grouped in different clusters. Ten of investigated Lithuanian CPV VP2 sequences were closely associated with CPV 2a antigenic variant (99.4% nt identity). Five CPV VP2 sequences from Lithuania were related to CPV-2a, but were rather divergent (6.8 nt differences). Only one CPV VP2 sequence from Lithuania was associated (99.3% nt identity) with CPV-2b VP2 sequences from France, Italy, USA and Korea. The four of eleven investigated Lithuanian dogs with CPV infection symptoms were vaccinated with CPV-2 vaccine, but their VP2 sequences were phylogenetically distantly associated with CPV vaccine strains VP2 sequences (11.5-15.8 nt differences).Ten Lithuanian CPV VP2 sequences had monophyletic relations among the close geographically associated samples, but five of them were rather divergent (1.0% less sequence similarity). The one Lithuanian CPV VP2 sequence was closely related with CPV-2b antigenic variant. All the Lithuanian CPV VP2 partial sequences were conservative and phylogenetically low associated with most commonly used CPV vaccine strains.

show abstract

Typing of canine parvovirus strains circulating in Brazil between 2008 and 2010

Cited by 53 publications

References 22 publications

A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

A phylogenetic study of canine parvovirus type 2c in midwestern Brazil

Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in São Paulo State, Brazil

Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples

Contact Info

Product

Resources

About