Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone in Hyphomicrobium denitrificans FJNU-6

Liu, Mengsu; Yang, Xinwei; Ren, Yang; Xia, Huaping; Huang, Jianzhong; Ke, Chongrong

doi:10.1007/s00253-020-10690-0

Cited by 6 publications

(14 citation statements)

References 31 publications

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“…Numerous effective biological strategies are suggested to improve the yield of secondary metabolites by a microorganism, such as medium component optimization, temperature regulation, oxygen concentration regulation, and others. [8][9][10] Microbial growth and secondary metabolite biosynthesis were significantly regulated by metal ions as suggested in the literature. Luo et al reported an effective strategy of the metal ions (Zn 2+ , Cu 2+ , and Mg 2+ ) coaddition could enhance the production of botrallin and TMC-264 by Hyalodendriella sp.…”

Section: Introductionmentioning

confidence: 80%

“…There were many biological control strategies to enhance the product of secondary metabolites by microbiol, such as optimization of nutritional components, regulation of temperature, control of oxygen concentration and others' regulation [8][9][10]. The effects of metal ions on growth of microbe and biosynthesis of secondary metabolites were evident in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Induced effect of Ca²⁺ and Al³⁺ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

Liu

Chen²,

Chen

et al. 2022

Biotech and App Biochem

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Chaetominine (CHA), an alkaloid with biological activity produced by Aspergillus fumigatus CY018, has strong anticancer activity against the human leukemia cell. However, its further research for pharmacokinetics is limited by CHA production in submerged fermentation, which is a problem urgently awaiting solutions by effective biological strategies. In this work, Ca 2+ and Al 3+ exhibited a strong promoting effect on CHA production after multiple metal ions screening. Then, the concentration of Ca 2+ and Al 3+ and the addition time was optimized. The suitable conditions were 0.5 mM Ca 2+ and 0.5 mM Al 3+ at 120 h, in which the CHA production reached 58.5 and 49.45 mg/L with optimized condition of Ca 2+ and Al 3+ respectively. The intermediate metabolites, such as DAHP, chorismate and tryptophan, were increased obviously with addition of Ca 2+ and Al 3+ . The activities of key enzymes of DAHPs, AroAs and TrpCs in CHA biosynthesis pathway were improved by 3.58-, 3.60-and 3.34-folds respectively. Meanwhile, the transcription levels of laeA, dahp, cs, and trpC were upregulated by 3.22-, 12.65-, 5.58-and 6.99-fold respectively by addition of Ca 2+ and Al 3+ . Additionally, the fermentation strategy was successfully scaled up to 5 L reactor, in which CHA production could attain 75.6 mg/L at 336 h. This work demonstrated that Ca 2+ and Al 3+ co-addition was an effective strategy for increasing CHA production, and the information obtained might be useful to the further improvement of CHA fermentation on large scale.

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Section: Introductionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Induced effect of Ca²⁺ and Al³⁺ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

Liu

Chen²,

Chen

et al. 2022

Biotech and App Biochem

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“…Using M. extorquens I–F2 and integrating atmospheric and room temperature plasma mutagenesis, flow cytometry, and high-throughput screening strategies, with other process enhancements, the titer of 1 was almost doubled . A two-stage oxygenation system with delayed continuous methanol supply increased levels of PQQ up to 1070 mg/L and 7.6 mg/L/h in cultures of Hyphomicrobium denitrificans FJNU-6 …”

Section: Biosynthesismentioning

confidence: 99%

“…280 A two-stage oxygenation system with delayed continuous methanol supply increased levels of PQQ up to 1070 mg/L and 7.6 mg/L/h in cultures of Hyphomicrobium denitrif icans FJNU-6. 281 The amino sugar 6-deoxy-6-(N-hydroxyethyl)amino-α-Lsorbofuranose (6NSL) (33) is an important intermediate in the biosynthesis of the antidiabetic drug miglitol (34) by G. oxidans 282,283 and is produced through the action of a membrane-bound, PQQ-dependent D-sorbitol dehydrogenase (mSLDH) in the periplasmic space. 278,284 A 5400 bp fragment from the G. oxidans H24 DNA library comprising the genes pqqABCDE showed good homology with other biosynthetic sequences for 1, 285 whose availability limits the formation of 35.…”

Section: Biosynthesismentioning

confidence: 99%

Pyrroloquinoline Quinone Chemistry, Biology, and Biosynthesis

Cordell

Daley²

2022

Chem. Res. Toxicol.

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The widely distributed, essential redox factor pyrroloquinoline quinone (PQQ, methoxatin) (1) was discovered in the mid-1960s. The breadth and depth of its biological effects are steadily being revealed, and understanding its biosynthesis at the genomic level is a continuing process. In this review, aspects of the chemistry, biology, biosynthesis, and commercial production of 1 at the gene level, and some applications, are presented from discovery through to mid-2021.

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“…Therefore, further improvement of CHA production is urgently required considering its potential application in the eld of medicine. There were many biological control strategies to enhance the product of secondary metabolites by microbiol, such as optimization of nutritional components, regulation of temperature, control of oxygen concentration and others' regulation [8][9][10]. The effects of metal ions on growth of microbe and biosynthesis of secondary metabolites were evident in the literature.…”

Section: Introductionmentioning

confidence: 99%

Induced effect of Ca2+ and Al3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

Liu

Chen²,

Chen

et al. 2021

Preprint

View full text Add to dashboard Cite

Chaetominine (CHA), an alkaloid with biological activity produced by Aspergillus fumigatus CY018, has strong anticancer activity against the human leukemia cell. However, its further research for pharmacokinetics is limited by CHA production in submerged fermentation, which is a problem urgently awaiting solutions by effective biological strategies. In this work, Ca2+ and Al3+ exhibited a strong promoting effect on CHA production after multiple metal ions screening. Then, the concentration of Ca2+ and Al3+ and the addition time was optimized. The suitable conditions were 0.5 mM Ca2+ and 0.5 mM Al3+ at 120 h, in which the CHA production reached 58.5 and 49.45 mg/L with optimized condition of Ca2+ and Al3+ respectively. The intermediate metabolites, such as DAHP, chorismate and tryptophan, were increased obviously with addition of Ca2+ and Al3+. The activities of key enzymes of DAHPs, AroAs and TrpCs in CHA biosynthesis pathway were improved by 3.58-, 3.60- and 3.34-folds respectively. Meanwhile, the transcription levels of laeA, dahp, cs, and trpC were upregulated by 3.22-, 12.65-, 5.58- and 6.99-fold respectively by addition of Ca2+ and Al3+. Additionally, the fermentation strategy was successfully scaled up to 5 L reactor, in which CHA production could attain 75.6 mg/L at 336 h. This work demonstrated that Ca2+ and Al3+ co-addition was an effective strategy for increasing CHA production, and the information obtained might be useful to the further improvement of CHA fermentation on large scale.

show abstract

Two-stage oxygen supply strategy for enhancing fed-batch production of pyrroloquinoline quinone in Hyphomicrobium denitrificans FJNU-6

Cited by 6 publications

References 31 publications

Induced effect of Ca²⁺ and Al³⁺ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

Induced effect of Ca²⁺ and Al³⁺ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

Pyrroloquinoline Quinone Chemistry, Biology, and Biosynthesis

Induced effect of Ca2+ and Al3+ on chaetominine synthesis by Aspergillus fumigatus CY018 under submerged fermentation

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