Two Receptor-Like Kinases Required for Arabidopsis Endodermal Root Organisation Shape the Rhizosphere Microbiome

Dürr, Julius; Reyt, Guilhem; Spaepen, Stijn; Hilton, Sally; Meehan, Cathal; Wu, Qi; Kamiya, Takehiro; Flis, Paulina; Dickinson, H. G.; Fehér, Attila; Bending, Gary D.; Schulze‐Lefert, Paul; Salt, David E.; Gutierrez-Marcos, José

doi:10.2139/ssrn.3475205

Cited by 3 publications

(4 citation statements)

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“…Knowing that UCC1 localizes at the CS ( Figures 2 C–2F) and mutations in UCC1 and UCC2 cause a strong defect in root apoplastic permeability ( Figures 1 C and S1 B), we looked at lignin deposition at the CS in ucc1&2 mutants ( Figure S4 A). Mutations in UCC1 and/or UCC2 do not cause an obvious disruption in the CS as observed in casp1casp3 , esb1 , sgn3 ( Figure S4 A), and other previously identified CS mutants, where clear gaps can be observed [ 3 , 5 , 6 , 7 , 11 , 13 , 14 , 15 , 33 ]. Further, there is no additivity between ucc1 , esb1 , and casp1casp3 observed for the deposition of ectopic lignin ( Figure S4 A).…”

Section: Resultssupporting

confidence: 55%

“…Of vital importance to this function are Casparian strips (CSs), which are belt-like lignin structures surrounding each endodermal cell, that interlock to form a barrier to diffusion in the apoplast [ 9 , 10 ]. This barrier is thought to enable the endodermis to exert control over uptake of water and solutes from the environment into the plant and perhaps also to control biotic interactions [ 1 , 11 ].…”

Section: Resultsmentioning

confidence: 99%

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Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips

et al. 2020

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Summary Casparian strips (CSs) are cell wall modifications of vascular plants restricting extracellular free diffusion into and out of the vascular system [ 1 ]. This barrier plays a critical role in controlling the acquisition of nutrients and water necessary for normal plant development [ 2 , 3 , 4 , 5 ]. CSs are formed by the precise deposition of a band of lignin approximately 2 μm wide and 150 nm thick spanning the apoplastic space between adjacent endodermal cells [ 6 , 7 ]. Here, we identified a copper-containing protein, Uclacyanin1 (UCC1), that is sub-compartmentalized within the CS. UCC1 forms a central CS nanodomain in comparison with other CS-located proteins that are found to be mainly accumulated at the periphery of the CS. We found that loss-of-function of two uclacyanins ( UCC1 and UCC2 ) reduces lignification specifically in this central CS nanodomain, revealing a nano-compartmentalized machinery for lignin polymerization. This loss of lignification leads to increased endodermal permeability and, consequently, to a loss of mineral nutrient homeostasis.

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Section: Resultssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 99%

Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips

et al. 2020

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“…In roots, the vasculature is surrounded by a barrier‐containing cell layer termed the endodermis. Only within the recent years have we gained molecular insight into the formation and function of the barriers established in the endodermis (Barberon et al ., 2016; Li et al ., 2017) coordinating root association and communication with the environment (Durr et al ., 2019; Holbein et al ., 2019; Liu et al ., 2019). Dependent on the stage of root development, the endodermis deploys different barrier systems to protect and isolate the vasculature and its long‐distance transport capacity.…”

Section: Introductionmentioning

confidence: 99%

The endodermal passage cell – just another brick in the wall?

2021

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The endodermis surrounds and protects the vasculature partly by depositing hydrophobic suberin in the cell walls. Yet, some cells remain unsuberised. These historically termed 'passage cells' are assumed to provide a low-resistance pathway to the xylem. Only recently have we started to gain molecular insights into these cells, which allow us to probe how roots coordinate communication with the environment across barriers with single-cell precision. Increased understanding of root physiology at a high-resolution is intriguing, as it is likely to provide us with new tools to improve overall plant health. With this in mind, we here provide a brief overview of passage cells, their presence across plant species, as well as a molecular update and future directions for passage cell-related research.

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“…Moreover, Arabidopsis plants lacking AtRLCK VI_A3 are characterized by a slightly higher susceptibility toward infection by the virulent powdery mildew fungus Erisyphe cruciferarum , indicating that either AtROP6 might signal through AtRLCK VI_A3 during pathogen response or AtRLCK VI_A3 has a HvRBK1-like function in regulating AtROPs [ 124 ]. Most recently, AtRLCK VI_A3 and AtRBK1 have been shown to function in casparian strip organization and shaping the root-associated microbiome [ 125 ], which indicates the general complexity of ROP-regulated plant–microbe interactions. Similar to OsRAC1 and HvRACB, AtROP6 might be involved in MTI by directly interacting with Arabidopsis respiratory burst oxidase homolog D (AtRBOHD).…”

Section: Rops Involved In Further Plant-microbe Interactionsmentioning

confidence: 99%

Regulation and Functions of ROP GTPases in Plant–Microbe Interactions

Engelhardt

Trutzenberg

Hückelhoven

2020

Cells

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Rho proteins of plants (ROPs) form a specific clade of Rho GTPases, which are involved in either plant immunity or susceptibility to diseases. They are intensively studied in grass host plants, in which ROPs are signaling hubs downstream of both cell surface immune receptor kinases and intracellular nucleotide-binding leucine-rich repeat receptors, which activate major branches of plant immune signaling. Additionally, invasive fungal pathogens may co-opt the function of ROPs for manipulation of the cytoskeleton, cell invasion and host cell developmental reprogramming, which promote pathogenic colonization. Strikingly, mammalian bacterial pathogens also initiate both effector-triggered susceptibility for cell invasion and effector-triggered immunity via Rho GTPases. In this review, we summarize central concepts of Rho signaling in disease and immunity of plants and briefly compare them to important findings in the mammalian research field. We focus on Rho activation, downstream signaling and cellular reorganization under control of Rho proteins involved in disease progression and pathogen resistance.

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Two Receptor-Like Kinases Required for Arabidopsis Endodermal Root Organisation Shape the Rhizosphere Microbiome

Cited by 3 publications

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Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips

Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips

The endodermal passage cell – just another brick in the wall?

Regulation and Functions of ROP GTPases in Plant–Microbe Interactions

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