Two-photon microscopy imaging of oxidative stress in human living erythrocytes

Tsakanova, Gohar; Arakelova, Elina; Ayvazyan, Violetta; Ayvazyan, Anna; Tatikyan, S. Sh.; Aroutiounian, Rouben; Dalyan, Yeva B.; Haroutiunian, Samvel G.; Tsakanov, V.; Arakelyan, Arsen

doi:10.1364/boe.8.005834

Cited by 10 publications

(8 citation statements)

References 48 publications

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“…The oxidative stress inside living RBCs was determined via the two-photon laser scanning microscopy imaging described earlier [ 81 , 82 , 88 ]. RBCs were isolated from the fresh blood samples using isotonic phosphate-buffered saline solution [ 81 , 82 , 88 , 89 , 90 , 91 ] and used for two-photon microscopy imaging immediately to avoid any alterations in the erythrocyte morphology.…”

Section: Methodsmentioning

confidence: 99%

The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats

Tsakanova

Avetisyan

Каралова

et al. 2022

IJMS

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Anemia is a commonly observed consequence of whole-body exposure to a dose of X-ray or gamma irradiation of the order of the mean lethal dose in mammals, and it is an important factor for the determination of the survival of animals. The aim of this study was to unravel the effect of laser-driven ultrashort pulsed electron beam (UPEB) irradiation on the process of erythropoiesis and the redox state in the organism. Wistar rats were exposed to laser-driven UPEB irradiation, after which the level of oxidative stress and the activities of different antioxidant enzymes, as well as blood smears, bone marrow imprints and sections, erythroblastic islets, hemoglobin and hematocrit, hepatic iron, DNA, and erythropoietin levels, were assessed on the 1st, 3rd, 7th, 14th, and 28th days after irradiation. Despite the fact that laser-driven UPEB irradiation requires quite low doses and repetition rates to achieve the LD50 in rats, our findings suggest that whole-body exposure with this new type of irradiation causes relatively mild anemia in rats, with subsequent fast recovery up to the 28th day. Moreover, this novel type of irradiation causes highly intense processes of oxidative stress, which, despite being relatively extinguished, did not reach the physiologically stable level even at the 28th day after irradiation due to the violations in the antioxidant system of the organism.

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Section: Methodsmentioning

confidence: 99%

The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats

Tsakanova

Avetisyan

Каралова

et al. 2022

IJMS

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“…Common organic fluorophores, such as fluorescein derivatives, have been modified with ROS-sensitive moieties that quench fluorescence; however, on ROS-mediated cleavage they possess fluorescence suitable for TPEF (26). Furthermore, the team of Lewis recently developed an azulene fluorophore for TPEF bioimaging of RNS and ROS (27).…”

Section: Phosphinatesmentioning

confidence: 99%

Advances in Imaging Reactive Oxygen Species

Espinoza

Røise

et al. 2020

J Nucl Med

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Reactive oxygen species (ROS) play a pivotal role in many cellular processes and can be either beneficial or harmful. The design of ROS-sensitive fluorophores has allowed for imaging of specific activity and has helped elucidate mechanisms of action for ROS. Understanding the oxidative role of ROS in the many roles it plays allows us to understand the human body. This review provides a concise overview of modern advances in the field of ROS imaging. Indeed, much has been learned about the role of ROS throughout the years; however, it has recently been shown that using nanoparticles, rather than individual small organic fluorophores, for ROS imaging can further our understanding of ROS.

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“…Oxidative status is generally assessed in erythrocytes, tissues, and plasma; however, because erythrocytes are exposed to biochemical damage and can be easily collected, they are frequently analyzed . Oxidative stress in erythrocytes has also been the focus of research in cats with DM.…”

Section: Introductionmentioning

confidence: 99%

“…14,15 Oxidative status is generally assessed in erythrocytes, tissues, and plasma; however, because erythrocytes are exposed to biochemical damage and can be easily collected, they are frequently analyzed. 16,17 Oxidative stress in erythrocytes has also been the focus of research in cats with DM. The activity of superoxide dismutase in erythrocytes is lower in diabetic cats than in controls and does not increase during treatment.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative status of erythrocytes, hyperglycemia, and hyperlipidemia in diabetic cats

Zini

Gabai

Salesov

et al. 2020

Veterinary Internal Medicne

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Background Erythrocytes of diabetic cats have decreased superoxide dismutase activity, possibly indicative of oxidative stress. Hypothesis Erythrocytes of diabetic cats undergo oxidative stress, which is caused by hyperglycemia and hyperlipidemia, and improves with treatment. Animals Twenty‐seven client‐owned cats with diabetes mellitus, 11 matched healthy cats, and 21 purpose‐bred healthy cats. Methods Prospective study. Advanced oxidized protein products, carbonyls (protein oxidation by‐products), and thiols (antioxidants) were quantified in erythrocyte membrane, thiobarbituric acid reactive substances (TBAR, lipid peroxidation by‐products), and thiols in erythrocyte cytoplasm of all cats. Comparison were performed between diabetic and matched healthy cats, between diabetic cats achieving remission or not, and among purpose‐bred cats after 10 days of hyperglycemia (n = 5) or hyperlipidemia (n = 6) versus controls treated with saline (n = 5) or untreated (n = 5). Results Compared with controls, erythrocytes of diabetic cats initially had higher median membrane carbonyls (4.6 nmol/mg total protein [range: 0.1‐37.7] versus 0.7 [0.1‐4.7], P < .001) and lower cytoplasmic TBAR (1.9 nmol/mg [0.5‐2.4] versus 2.4 [1.4‐3.5] P < .001), and thiols (419 nmol/mg [165‐621] versus 633 [353‐824], P < 0.001). After 12‐16 weeks of treatment in diabetic cats, carbonyls decreased by 13% (P < .001), but remained higher (P < .001) and TBAR and thiols lower (P = .02, P < .001) than those in controls. No differences were observed between diabetic cats achieving remission or not, and among purpose‐bred cats. Conclusions and Clinical Importance Diabetes mellitus is associated with increased protein oxidation and reduced antioxidant defenses, which persist during treatment and remission, although mild improvement in protein oxidation occurs. Short‐term hyperglycemia or hyperlipidemia does not cause oxidative stress. The reason for decreased TBAR remains unknown.

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Two-photon microscopy imaging of oxidative stress in human living erythrocytes

Cited by 10 publications

References 48 publications

The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats

The Effect of Low-Energy Laser-Driven Ultrashort Pulsed Electron Beam Irradiation on Erythropoiesis and Oxidative Stress in Rats

Advances in Imaging Reactive Oxygen Species

Oxidative status of erythrocytes, hyperglycemia, and hyperlipidemia in diabetic cats

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