Two-Photon Microscopy as a Tool to Study Blood Flow and Neurovascular Coupling in the Rodent Brain

Shih, Andy Y.; Driscoll, Jonathan D.; Drew, Patrick J.; Nishimura, Nozomi; Schaffer, Chris B.; Kleinfeld, David

doi:10.1038/jcbfm.2011.196

Cited by 398 publications

(373 citation statements)

References 203 publications

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“…The effect of isoflurane on vasodynamics of individual arterioles was ascertained with two-photon imaging of thinned-skull, head-fixed animals [58].…”

Section: (E) Single Vessel Imagingmentioning

confidence: 99%

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Precision mapping of the vibrissa representation within murine primary somatosensory cortex

Knutsen

Matéo

Kleinfeld

2016

Phil. Trans. R. Soc. B

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The ability to form an accurate map of sensory input to the brain is an essential aspect of interpreting functional brain signals. Here, we consider the somatotopic map of vibrissa-based touch in the primary somatosensory (vS1) cortex of mice. The vibrissae are represented by a Manhattan-like grid of columnar structures that are separated by inter-digitating septa. The development, dynamics and plasticity of this organization is widely used as a model system. Yet, the exact anatomical position of this organization within the vS1 cortex varies between individual mice. Targeting of a particular column in vivo therefore requires prior mapping of the activated cortical region, for instance by imaging the evoked intrinsic optical signal (eIOS) during vibrissa stimulation. Here, we describe a procedure for constructing a complete somatotopic map of the vibrissa representation in the vS1 cortex using eIOS. This enables precise targeting of individual cortical columns . We found, using C57BL/6 mice, that although the precise location of the columnar field varies between animals, the relative spatial arrangement of the columns is highly preserved. This finding enables us to construct a canonical somatotopic map of the vibrissae in the vS1 cortex. In particular, the position of any column, in absolute anatomical coordinates, can be established with near certainty when the functional representations in the vS1 cortex for as few as two vibrissae have been mapped with eIOS. This article is part of the themed issue ‘Interpreting BOLD: a dialogue between cognitive and cellular neuroscience’.

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“…The effect of isoflurane on vasodynamics of individual arterioles was ascertained with two-photon imaging of thinned-skull, head-fixed animals [58].…”

Section: (E) Single Vessel Imagingmentioning

confidence: 99%

“…Imaging with acclimated and head-fixed mice was performed as described and illustrated [36,58]. Anaesthetized mice were maintained at a constant body temperature of 378C using a temperature control system (no.…”

Section: (C) Intrinsic Optical Signal Imagingmentioning

confidence: 99%

Precision mapping of the vibrissa representation within murine primary somatosensory cortex

Knutsen

Matéo

Kleinfeld

2016

Phil. Trans. R. Soc. B

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“…At each imaging session, we visualized endogenous tdTomato or YFP fluorescence from pericytes and green or red fluorescence from the vasculature, which was labeled through an intravenous injection of FITC-dextran or Texas red-dextran (i.v. dye), respectively (Shih et al, 2012). …”

Section: Resultsmentioning

confidence: 99%

“…Our procedures for in vivo two-photon imaging have been described previously (Shih et al, 2012). Additional details are provided in Supplemental Experimental Procedures.…”

Section: Methodsmentioning

confidence: 99%

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Dynamic Remodeling of Pericytes In Vivo Maintains Capillary Coverage in the Adult Mouse Brain

et al. 2018

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SUMMARY Direct contact and communication between pericytes and endothelial cells is critical for maintenance of cerebrovascular stability and blood-brain barrier function. Capillary pericytes have thin processes that reach hundreds of micrometers along the capillary bed. The processes of adjacent pericytes come in close proximity but do not overlap, yielding a cellular chain with discrete territories occupied by individual pericytes. Little is known about whether this pericyte chain is structurally dynamic in the adult brain. Using in vivo two-photon imaging in adult mouse cortex, we show that while pericyte somata were immobile, the tips of their processes underwent extensions and/or retractions over days. The selective ablation of single pericytes provoked exuberant extension of processes from neighboring pericytes to contact uncovered regions of the endothelium. Uncovered capillary regions had normal barrier function, but were dilated until pericyte contact was regained. Pericyte structural plasticity may be critical for cerebrovascular health and warrants detailed investigation.

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Importance of Preclinical Research in the Development of Neuroprotective Strategies for Ischemic Stroke

et al. 2014

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Two-Photon Microscopy as a Tool to Study Blood Flow and Neurovascular Coupling in the Rodent Brain

Cited by 398 publications

References 203 publications

Precision mapping of the vibrissa representation within murine primary somatosensory cortex

Precision mapping of the vibrissa representation within murine primary somatosensory cortex

Dynamic Remodeling of Pericytes In Vivo Maintains Capillary Coverage in the Adult Mouse Brain

Importance of Preclinical Research in the Development of Neuroprotective Strategies for Ischemic Stroke

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