Two Odorant-Binding Proteins Involved in the Recognition of Sex Pheromones in <i>Spodoptera litura</i> Larvae

Ma, Sai; Li, Lu−Lu; Yao, Wei-Chen; Yin, Mao-Zhu; Li, Jianqiao; Xu, Junwei; Dewer, Youssef; Zhu, Xiu-Yun; Zhang, Ya-Nan

doi:10.1021/acs.jafc.2c04335

Cited by 16 publications

(25 citation statements)

References 47 publications

(90 reference statements)

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“…RNAi is a reliable technology currently used to explore the exact physiological function of key genes in living insects. Recently, it has been successfully applied in a variety of noctuid pests by injecting siRNA into the body, such as Spodoptera litura 50 and Mythimna separata. 51 We injected siCSP2 into male A. lepigone, and the silencing rate 24 h after treatment was 98.29%, but it has no effect on the expression level of other ten olfactory genes in male moths, indicating that we successfully knockdown AlepCSP2 to a large extent, and siCSP2 can target the silencing of target gene expression without affecting other olfactory genes.…”

Section: ■ Discussionmentioning

confidence: 99%

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Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles

Zhu

Yao

et al. 2023

J. Agric. Food Chem.

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In moths, the interactions between chemosensory proteins (CSPs) and sex pheromones have yet to be comprehensively investigated. Here, we examined the function of AlepCSP2 in male Athetis lepigone based on protein expression, molecular docking, site-directed mutagenesis, fluorescence competitive binding analyses, and RNA interference (RNAi) experiments. We found that AlepCSP2 showed strong binding affinity for two sex pheromones and five maize volatiles and that binding was optimal under neutral conditions. Furthermore, we identified six amino acids as being key residues involved in the interaction between AlepCSP2 and multiple ligands. Further RNAi showed that siCSP2 males displayed consistently lower electroantennography responses to two sex pheromones and three maize volatiles at different dosages tested, and the mating rate also decreased significantly by 37.50%. These findings will contribute to characterizing the binding mechanisms of moth CSPs to sex pheromones and host volatiles and also identify unique targets for developing novel pest behavior disruptors.

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Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles

Zhu

Yao

et al. 2023

J. Agric. Food Chem.

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“…Previous reports on Spodoptera OBPs provides a blueprint for the prediction of SlitOBP ligands based on the interaction of phylogeny and chemical structure. For example, larval SlitGOBP 2 of S. litura have been reported to function in sex pheromone recognition [ 83 ]. In the same species, OBP 11 displays strong binding with sex pheromones [ 84 ] and CSP 8 can bind with the oviposition deterrent chemical rhodojaponin III [ 85 ].…”

Section: Discussionmentioning

confidence: 99%

Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides

Jia

Mohamed

Cattaneo

et al. 2023

IJMS

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Spodoptera frugiperda is a worldwide generalist pest with remarkable adaptations to environments and stresses, including developmental stage-related behavioral and physiological adaptations, such as diverse feeding preferences, mate seeking, and pesticide resistance. Insects’ odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are essential for the chemical recognition during behavioral responses or other physiological processes. The genome-wide identification and the gene expression patterns of all these identified OBPs and CSPs across developmental stage-related S. frugiperda have not been reported. Here, we screened for genome-wide SfruOBPs and SfruCSPs, and analyzed the gene expression patterns of SfruOBPs and SfruCSPs repertoires across all developmental stages and sexes. We found 33 OBPs and 22 CSPs in the S. frugiperda genome. The majority of the SfruOBP genes were most highly expressed in the adult male or female stages, while more SfruCSP genes were highly expressed in the larval or egg stages, indicating their function complementation. The gene expression patterns of SfruOBPs and SfruCSPs revealed strong correlations with their respective phylogenic trees, indicating a correlation between function and evolution. In addition, we analyzed the chemical-competitive binding of a widely expressed protein, SfruOBP31, to host plant odorants, sex pheromones, and insecticides. Further ligands binding assay revealed a broad functional related binding spectrum of SfruOBP31 to host plant odorants, sex pheromones, and insecticides, suggesting its potential function in food, mate seeking, and pesticide resistance. These results provide guidance for future research on the development of behavioral regulators of S. frugiperda or other environmentally friendly pest-control strategies.

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“…12,22 Some studies reported that larvae can recognize certain pheromones, 34 even sexual ones. 35 However, there were relatively few investigations of larval OBPs in host plant recognition. Because most lepidopteran larvae graze directly on numerous crops, studying the roles of larval OBPs is critical.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Olfaction is vital in the transfer of chemical information and has long been studied by entomologists. OBPs are chemical signal recognition carriers, which are required for signal receiving while looking for food, mates, and so on. , Some studies reported that larvae can recognize certain pheromones, even sexual ones . However, there were relatively few investigations of larval OBPs in host plant recognition.…”

Section: Discussionmentioning

confidence: 99%

The Odorant-Binding Protein 1 Mediates the Foraging Behavior of Grapholita molesta Larvae

Tan,

Wei,

et al. 2023

J. Agric. Food Chem.

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Since eggs are laid directly on fruit skin, it is typically believed that food odor has little impact on the foraging of Grapholita molesta larvae. It is crucial to note that larvae that hatch on twigs and leaves could need some sort of identification system when foraging. Here, 22 GmolOBP genes were identified from the G. molesta larval transcriptome via the comparison of conserved domain and homology in the protein level. GmolOBP1 had strong affinities for important pear-fruit volatiles, which caused larvae strong behavioral responses. However, after GmolOBP1 silencing, the larvae lost their attraction to methyl salicylate, α-farnesene, butyl acetate, ethyl butanoate, and ethyl hexanoate, and the effects of larvae seeking various pears were significantly reduced. Consequently, GmolOBP1 was required for the reception of pear volatiles and was involved in mediating how G. molesta larvae foraged. Our research revealed the GmolOBP1 foraging signal recognition mechanism as well as potential molecular targets for field pest management.

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Two Odorant-Binding Proteins Involved in the Recognition of Sex Pheromones in Spodoptera litura Larvae

Cited by 16 publications

References 47 publications

Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles

Chemosensory Protein 2 of Male Athetis lepigone Is Involved in the Perception of Sex Pheromones and Maize Volatiles

Odorant-Binding Proteins and Chemosensory Proteins in Spodoptera frugiperda: From Genome-Wide Identification and Developmental Stage-Related Expression Analysis to the Perception of Host Plant Odors, Sex Pheromones, and Insecticides

The Odorant-Binding Protein 1 Mediates the Foraging Behavior of Grapholita molesta Larvae

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