Two matrix metalloproteinases inhibitors from Ferula persica var. persica

Shahverdi, A R; Saadat, Farshid; Khorramizadeh, Mohammad Reza; Iranshahi, Mehrdad; Khoshayand, Mohammad-Reza

doi:10.1016/j.phymed.2006.01.003

Cited by 56 publications

(44 citation statements)

References 8 publications

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“…The chemistry of this genus has been studied by many investigators and is well documented as a good source of biologically active compounds such as sesquiterpene derivatives (Ahmed et al, 2001;Ahmed, 1999;Valle et al, 1987;Iranshahi et al, 2004bIranshahi et al, , 2007Iranshahi et al, , 2008Shahverdi et al, 2006). Sesquiterpene derivatives, especially sesquiterpene coumarins, were stored in the roots of the plants; therefore the roots are a better source for isolating sesquiterpene coumarins than the aerial parts.…”

Section: Introductionmentioning

confidence: 99%

New sesquiterpene coumarins from the roots ofFerula flabelliloba

Iranshahi

Kalategi

Sahebkar

et al. 2010

Pharmaceutical Biology

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Section: Introductionmentioning

confidence: 99%

New sesquiterpene coumarins from the roots ofFerula flabelliloba

Iranshahi

Kalategi

Sahebkar

et al. 2010

Pharmaceutical Biology

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“…This method was carried out to assess the potential effects of plants extracts on cell viability according to the published data (Shahverdi et al, 2006). After each experiment, cells were washed three times with ice-cold phosphate buffered saline (PBS), pH 7.2, followed by fi xation in 5 % formaldehyde (Merck, Germany) solution.…”

Section: Colorimetric Cytotoxicity Assaymentioning

confidence: 99%

Concomitant chemopreventive and antibacterial effects of some Iranian plants from the genus Cousinia (Asteraceae)

Shahverdi¹,

Khorramizadeh²,

Attar³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Efeitos quimiopreventivo e antibacteriano concomintantes de algumas plantas iranianas do gênero Cousinia (Asteraceae)". Durante os últimos anos, várias espécies de Cousinia (Asteraceae) têm sido identifi cadas no Irã. No entanto, dados acerca de suas atividades biológicas permanecem limitados. O principal propósito desta pesquisa foi avaliar a potencial citotoxidade e os efeitos inibitórios de metaloproteinases da matriz (MMP) de sete extratos etanólicos de Cousinia utilizando um modelo de linhagem celular (Fibrosarcoma-WEHI 164). Além disso, investigamos a atividade antibacteriana destes extratos etanólicos de Cousinia, utilizando o método de difusão em disco. Dentre os extratos etanólicos, o extrato total de C. sulabadensis promoveu inibição signifi cativa da atividade de MMP de maneira dose-resposta (49,2 ± 0,51, p < 0,05). Todavia, este extrato exibiu o menor efeito de citotoxicidade em todas as concentrações testadas. A concentração necessária para produzir uma taxa de 50% de morte celular (IC50) com C. shulabadensis foi 304,5 ± 0,61 μg/mL. A IC50 calculada para a citotoxicidade dos outros extratos de espécies de Cousinia situou-se entre 18,4 ± 0,59 e 87,9 ± 0,58 μg/mL. A maior atividade antibacteriana foi observada para o extrato total de Cousinia phyllocephala. Em conclusão, este estudo corrobora que espécies de Cousinia mostram uma notável inibição da atividade de metaloproteinases da matriz. Os concomitantes efeitos inibitórios de MMP e baixa citotoxicidade observados em C. sulabadensis devem direcionar este extrato para futuros estudos de potenciais fi toterápicos anti-invasivos.Unitermos: Cousinia, citotoxicidade, IC50, zimografi a, antibacteriano, linhagem celular WEHI 164.ABSTRACT: During the past several years, various species of Cousinia (Asteraceae) have been authenticated in Iran. However, data concerning their biological activities remain limited. The main purpose of this research was to assess potential cytotoxicity and matrix metalloproteinases (MMP) inhibitory effects of seven ethanol extracts of Cousinia using a cell line model (Fibrosarcoma-WEHI 164). We further investigated the antibacterial activity of these Cousinia ethanol extracts, using disk diffusion method. Among the ethanol extracts, the total extract of C. sulabadensis elicited signifi cant inhibition of MMP activity in a dose-response fashion (49.2 ± 0.51, p < 0.05). However, this extract exhibited the lowest cytotoxicity effect at all tested concentrations. The concentration necessary to produce a 50% cell death rate (IC50) with C. shulabadensis was 304.5 ± 0.61 μg/mL. The calculated IC50 for cytotoxicity of the other Cousinia species extracts ranged between 18.4 ± 0.59 to 87.9 ± 0.58 μg/mL. The highest antibacterial activity was observed for the total extract of Cousinia phyllocephala. In conclusion, this study supports that Cousinia species display a remarkable inhibition of matrix metalloproteinases activity. The concomitant MMPinhibitory and low cytotoxicity effects observed in C. sulabadensis might coin this extra...

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“…Umbelliprenin (Umb; C 24 H 30 O 3 ; molecular weight, 366), a member of 7-prenyloxycoumarins, is a naturally occurring compound, which is isolated from Ferula species, including Ferula szowitsiana (1,2). This compound is able to suppress tumorigenesis through its anti-inflammatory, anti-genotoxicity, anti-invasive and lipoxygenase inhibitory activities (3)(4)(5)(6). In vitro, Umb has been reported to attenuate the proliferation of various cancer cell lines, and is able to prevent/delay cancer in in vivo animal models of papilloma and lung cancer (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Immune-associated proteins with potential in vivo anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach

et al. 2016

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Abstract. Umbelliprenin (Umb), a natural coumarin, has demonstrated anti-tumor activities, both in vitro and particularly in vivo, in several types of cancer, including lung cancer. The present study aimed to identify molecular targets of Umb using a high-throughput approach. Lung cancer cell lines, QU-DB (large-cell lung carcinoma) and A549 (adenocarcinoma), were treated with Umb. Differentially-expressed proteins were identified using two-dimensional electrophoresis coupled to mass spectrometry. In the QU-DB cells, differential expression of proteins, including downregulation of the tumorigenic protein heat shock protein 90 kDa and upregulation of the potential anti-tumor proteins Nipsnap1 and glycine-tRNA ligase (GRS), suggested that Umb is a strong anti-tumor compound. In the A549 cells, differential expression of proteins indicated possible contradictory effects of Umbregarding tumorigenesis, which included downregulation of the tumorigenic protein cyclophilin and the tumor suppressor MST, and upregulation of stathmin (tumorigenic) and calreticulin. Calreticulun, in addition to GRS in QU-DB cells, stimulates anti-tumor immune responses in vivo. To the best of our knowledge, the present study is the first to use a high-throughput approach to identify targets of Umb in cancer. These molecular targets suggested that Umb may exhibit stronger in vitro anti-tumor activity against the large-cell carcinoma model than the adenocarcinoma model. Furthermore, it has been reported that Umb exhibits higher cytotoxicity against QU-DB cells than A549 cells in vitro, and significant Umb anti-tumor activity against lung cancer in vivo, which is consistent with previously published literature. In each cell type, immune-associated molecules were upregulated, indicating that this naturally occurring compound exhibits marked anti-tumor activity in vivo. However, further studies that investigate the effect of Umb in different in vitro models of cancer are required.

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Two matrix metalloproteinases inhibitors from Ferula persica var. persica

Cited by 56 publications

References 8 publications

New sesquiterpene coumarins from the roots ofFerula flabelliloba

New sesquiterpene coumarins from the roots ofFerula flabelliloba

Concomitant chemopreventive and antibacterial effects of some Iranian plants from the genus Cousinia (Asteraceae)

Immune-associated proteins with potential in vivo anti-tumor activities are upregulated in lung cancer cells treated with umbelliprenin: A proteomic approach

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