Two-dimensional electrophoresis of plasma lipoproteins: Recognition of new apo A-I-containing subpopulations

Increased Plasma Lipid-Poor Apolipoprotein A-I in Patients with Coronary Artery Disease

“…The molecular weight of lipidpoor apo A-I in our study was almost 55 000, which is somewhat smaller than the 67 000 -75 000 reported previously (8 ), and the two-dimensional electrophoretic mobility of lipid-poor apo A-I was similar to that of pre-␤1-HDL (8,11 ). Plasma lipid-poor apo A-I was not stable, and its concentration increased with time during storage at 4°C.…”

The Farnesoid X-receptor Is an Essential Regulator of Cholesterol Homeostasis

“…Lipoproteins from pooled plasma samples (100 l) were separated by FPLC using two Superose 6HR 10/30 columns in series (Amersham Biosciences, Piscataway, NJ). High density lipoprotein (HDL) subclasses were resolved by two-dimensional gel electrophoresis (19). Plasma apoB was measured by enzyme-linked immunosorbent assay (20).…”

Evidence for the presence of lipid-free monomolecular apolipoprotein A-1 in plasma

“…Supplemental Material can be found at: than 1.21 g/ml ( 11 ). This fact suggests that the discoidal HDL in lymph is different from plasma pre ␤ 1-HDL.…”