Two different correlations between nanoindentation modulus and mineral content in the bone–cartilage interface

Gupta, Himadri S.; Schratter, Sonja; Tesch, W; Roschger, Paul; Berzlanovich, Andrea; Schoeberl, T.; Klaushofer, Klaus; Fratzl, Peter

doi:10.1016/j.jsb.2004.10.010

Cited by 201 publications

(176 citation statements)

References 35 publications

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“…Secondly, although the ovariectomised sheep is an established model of postmenopausal osteoporosis (Turner et al, 1995a;Turner et al, 1995b) ewes, unlike humans, have twenty oestrous cycles and healthy sheep experience a short period (1-2 months) of oestrogen defi ciency annually (Newton et al, 2004). Nonetheless, the calcium content values we measured in sheep bone tissue (22-26%) are comparable with ranges reported from control (18-28%) and osteoporotic human bone tissue (16-26%) (Roschger et al, 1998;Roschger et al, 2001;Gupta et al, 2005). We expect that differences in tissue heterogeneity would be more pronounced with larger sample sizes and in human osteoporotic bone compared to healthy subjects that do not experience an oestrogen defi cient period.…”

Section: Discussionsupporting

confidence: 83%

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Gleeson²,

Browne³

et al. 2011

eCM

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Heterogeneity of bone mineral during oestrogen defi ciency European Cells and Materials Vol. 21 2011 (pages 396-406) ISSN 1473-2262 AbstractAlthough osteoporosis reduces overall bone mass causing bone fragility, recent studies report that the remaining bone tissue is signifi cantly stiffer. Preliminary studies indicate that alterations in bone tissue mineral content might explain these changes, albeit that other studies report conflicting observations. The objective of this study is to quantify whether the distribution of bone tissue mineral is altered during oestrogen defi ciency. Individual trabeculae were harvested from the proximal femur of 7 ovariectomised sheep (OVX), sacrifi ced 12 months postsurgery, and 5 age-matched controls. Mineral content (wt% Ca) was determined using a quantitative backscattered scanning electron microscopy imaging approach. Mineral heterogeneity within individual trabeculae was compared by calculating the full width at half maximum (FWHM) of mineral density distributions. Mean calcium content, the spatial distribution of mineral within trabeculae and the inter-trabecular variation between regions of proximal femora were also compared. Oestrogen defi ciency increased mineral heterogeneity within individual trabeculae compared to healthy controls, as measured by FWHM (3.57 ± 0.68 vs. 3.17 ± 0.36 wt% Ca, p = 0.04). I n particular mineral variability increased between superficial and deep regions of trabeculae of OVX animals (p = 0.04). Interestingly, mineralisation variability between greater and lesser trochanters (i.e. intertrochanteric fracture line) was increased in OVX compared to CON, as indicated by a greater % difference in the standard deviation of trabecular mineral content (77.11 ± 11.70 vs. 45.64 ± 23.70 %, p = 0.03). Such changes are undetectable by evaluating the mean mineral content of bone tissue, but may contribute to changes in bone mechanical strength following osteoporotic bone loss.

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Section: Discussionsupporting

confidence: 83%

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Gleeson²,

Browne³

et al. 2011

eCM

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“…Others have shown the importance of the collagen matrix organization on bone mechanical properties [70,71] and recently we have shown using nanoindentation that compared to wild-type mice, oim have greater mineralization of a poorly organized matrix [72]. Therefore, exogenous cells may have affected the bone mineralization or improved organization of bone matrix collagen fibers in the oim bones at the microscopic matrix scale, potentially in response to production of normal COL1A2.…”

Section: Discussionmentioning

confidence: 96%

Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta

Jones

Moschidou

Abdulrazzak

et al. 2014

Stem Cells and Development

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Osteogenesis imperfecta (OI) is a genetic bone pathology with prenatal onset, characterized by brittle bones in response to abnormal collagen composition. There is presently no cure for OI. We previously showed that human first trimester fetal blood mesenchymal stem cells (MSCs) transplanted into a murine OI model (oim mice) improved the phenotype. However, the clinical use of fetal MSC is constrained by their limited number and low availability. In contrast, human fetal early chorionic stem cells (e-CSC) can be used without ethical restrictions and isolated in high numbers from the placenta during ongoing pregnancy. Here, we show that intraperitoneal injection of e-CSC in oim neonates reduced fractures, increased bone ductility and bone volume (BV), increased the numbers of hypertrophic chondrocytes, and upregulated endogenous genes involved in endochondral and intramembranous ossification. Exogenous cells preferentially homed to long bone epiphyses, expressed osteoblast genes, and produced collagen COL1A2. Together, our data suggest that exogenous cells decrease bone brittleness and BV by directly differentiating to osteoblasts and indirectly stimulating host chondrogenesis and osteogenesis. In conclusion, the placenta is a practical source of stem cells for the treatment of OI.

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“…The tidemark is a typical narrow seam of gradually increasing mineral content at the border between non-calcified and calcified articular cartilage. 24,25 Younger bone packets have a characteristic lower mineral content (lower grey level in BE images) than the older, more mature bone packets (higher grey level in BE images). 26 Because of this, BE imaging provides information on the histological features of the analysed bone sections, and was therefore performed on all samples prior to -XRF measurements.…”

Section: Backscattered Electron Imagingmentioning

confidence: 99%

Determination of the elemental distribution in human joint bones by SR micro XRF

et al. 2007

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In humans the toxic trace element lead (Pb) mainly accumulates in the skeletal tissue where it resides for a long period. Although Pb is known to play a role in bone and cartilage diseases, the distribution of Pb in these tissues is mostly unknown. Therefore synchrotron‐radiation‐induced micro x‐ray fluorescence analysis (SR µ‐XRF) in various geometries was used to determine the distributions of Pb, Ca, Zn and Sr at the cartilage‐bone interface in human femoral heads and patellae. SR µ‐XRF results were matched with backscattered electron (BE) images providing information on structural features of the tissue. Conventional SR µ‐XRF at HASYLAB beamline L showed that Pb mostly accumulates in a zone of some micrometers around the transition between non‐calcified and calcified cartilage. However, the relatively large sample thickness did not allow more precise conclusions. Exploiting the 3D capabilities of confocal SR µ‐XRF and SR microfluorescence tomography at ANKA Fluo‐Topo beamline and HASYLAB beamline L, the spatial resolution could be improved and a highly specific accumulation of Pb at the tidemark, the calcification front between non‐calcified and calcified cartilage, was detected. It was found that Pb and Zn accumulation coincide at the tidemark. Since the tidemark plays an important role in osteoarthritis, the results may strengthen other authors' conclusions that Pb is associated with this joint disease. Although offering less spatial resolution when compared to micro‐tomography, confocal SR µ‐XRF is best suited for such studies. It facilitates the comparison of element maps with other imaging techniques like BE imaging. Copyright © 2007 John Wiley & Sons, Ltd.

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Two different correlations between nanoindentation modulus and mineral content in the bone–cartilage interface

Cited by 201 publications

References 35 publications

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Potential of Human Fetal Chorionic Stem Cells for the Treatment of Osteogenesis Imperfecta

Determination of the elemental distribution in human joint bones by SR micro XRF

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