Two different cDNAs encoding TFIID proteins of maize

Haaß, Michael M.; Feix, Günter

doi:10.1016/0014-5793(92)80260-n

Cited by 31 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a mechanism is attractive and consistent with the evidence that the TATA motifs in eucaryotic cells are functionally heterogenous (16,58,(68)(69)(70). The TBP contains a highly conserved 180 amino acid C-terminal DNA binding domain and a nonconserved amino terminal region of variable length (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Genetic and biochemical analysis of the TBP indicate its function to be species specific (29,71,72).…”

Section: Discussionsupporting

confidence: 68%

“…The structure of this promoter is complex in that it contains two overlapping transcriptional control regions that direct transcription from the + 1 and -26 positions (15)(16)(17)(18). Both promoters lack the consensus TATA sequence, but have in its place sequence motifs TTAAGA and TAAATT, centered approximately 30 bp upstream of the +1 and -26 cap sites, respectively (15 -18).…”

Section: Introductionmentioning

confidence: 99%

“…In vitro and in vivo studies have shown that the two start sites of this dual promoter are indeed controlled by these two TATAlike sequences (15,18). Recently, the TBP gene has been cloned from a number of different organisms, ranging from yeast to humans (19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). In all these instances, only one TBP gene has been identified with the exception of maize (30) and Arabidopsis (26) in which two genes that encode TBP have been found.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Activation of a dual adenovirus promoter containing nonconsensus TATA motifs inSchizosaccharomyces pombe: role of TATA sequences in the efficiency of transcription

Swaminathan

Malhotra²,

Manohar³

et al. 1993

Nucl Acids Res

View full text Add to dashboard Cite

The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence TTAAGA provides the TATA box function for the +1 promoter, whereas a sequence TAAATT, with a closer resemblance to the consensus (TATAA/TA) provides this function for the -26 promoter. Yet, in human cells, the +1 promoter is transcribed about 20 fold more efficiently than the -26 promoter. We found that both promoters are transcribed faithfully in S. pombe with start sites identical or close to those found in human cells. Surprisingly, the relative ratio of expression for the +1 and -26 promoters was exactly reversed in S. pombe cells. This reversal appeared to be due to the relatively weak binding of S. pombe TATA binding protein to the TTAAGA motif, rather than to its rate of dissociation. Furthermore, we show that in S. pombe, promoter expression correlates well with the nucleotide sequence of the TATA element rather than the context in which it is placed. By contrast, it is the context of the TATA element, rather than its nucleotide sequence that appears to be critical for promoter expression in human cells. Our data suggest the existence of one or more additional factors in human cells that permit the utilization of nonconsensus TATA elements. S. pombe appears to lack these factors.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Activation of a dual adenovirus promoter containing nonconsensus TATA motifs inSchizosaccharomyces pombe: role of TATA sequences in the efficiency of transcription

Swaminathan

Malhotra²,

Manohar³

et al. 1993

Nucl Acids Res

View full text Add to dashboard Cite

show abstract

“…The basic transcription machinery appears to be highly conserved between plants and animals and may share a common mechanism of transcriptional activation (Haas and Feix, 1992;Mukumoto et al, 1993). For example, the yeast acidic transcription factor GAL4 was shown to activate transcription in plant (Ma et al, 1988), Drosophila (Fischer et al, 1988), and mammalian (Kakidani and Ptashne, 1988) cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of the nuclear factors EmBP1 and viviparous1 on the transcription of the Em gene in HeLa nuclear extracts.

Razik

Quatrano

1997

Plant Cell

View full text Add to dashboard Cite

Templates constructed from the wheat Em and maize rab28 promoters are efficiently and accurately transcribed in the well-characterized cell-free transcription system prepared from HeLa nuclei. Deletion analysis of the Em promoter indicates that a G-box (CACGTG) element (Em1 b) is required for transcription. USF, a Myc transcription factor in HeLa nuclear extracts, activates transcription by binding t o Emlb, as shown by the ability of an antibody raised against USF t o inhibit transcription and t o interfere with Em1 b complex formation in an electrophoretic mobility shift assay. The addition of the recombinant Viviparousl protein from maize t o HeLa nuclear extracts specifically stimulated transcription of the Em promoter but was dependent on the presence of USF in the extract. In USF-depleted extracts, the addition of recombinant EmBP1, a basic leucine zipper transcription factor from wheat, activated transcription through Em1 b as well as from a similar G-box in the adenovirus major late promoter. Our study demonstrates that the basic transcriptional apparatus in HeLa nuclear extract supports transcription from plant promoters and can be used t o assay the function of certain plant nuclear proteins, thereby helping t o determine their effects on transcription.

show abstract

Both Arabidopsis TATA binding protein (TBP) isoforms are functionally identical in RNA polymerase II and III transcription in plant cells: evidence for gene-specific changes in DNA binding specificity of TBP.

1993

View full text Add to dashboard Cite

Promoters of pol II and pol III transcribed U‐snRNA genes in plants have identical sequence elements comprised of a −30 TATA box and an upstream sequence element (USE), located four or three helical turns upstream of the TATA box in pol II and pol III genes, respectively; it is this difference in element spacing that determines the RNA polymerase specificity of the gene. We are interested in identifying factors binding to U‐snRNA gene promoters and their role in selection of RNA polymerase. In this work we have investigated possible differences in the activity of the two TATA binding proteins (TBPs) encoded by two different TBP genes of Arabidopsis. Using mutant TBPs with altered DNA binding specificity, similar to those described previously in yeast, we show that two Arabidopsis TBP isoforms are equally active with both pol II and pol III U‐snRNA genes and with an mRNA gene transfected into plant protoplasts. In contrast to yeast, where modified TBP permits transcription only from promoters containing the TGTAAA mutant of the consensus (TATAAA) TATA element, altered Arabidopsis TBPs also suppress other TATA box mutants. Similar results were obtained with human and yeast TBP mutants expressed in plant cells. Interestingly, in several cases suppression of different TATA box mutants by altered TBPs was gene or RNA polymerase specific suggesting that assembly of TBP into specific complexes containing different TBP‐associated factors may alter DNA binding specificity of the protein.

show abstract

Two different cDNAs encoding TFIID proteins of maize

Cited by 31 publications

References 29 publications

Activation of a dual adenovirus promoter containing nonconsensus TATA motifs inSchizosaccharomyces pombe: role of TATA sequences in the efficiency of transcription

Activation of a dual adenovirus promoter containing nonconsensus TATA motifs inSchizosaccharomyces pombe: role of TATA sequences in the efficiency of transcription

Effect of the nuclear factors EmBP1 and viviparous1 on the transcription of the Em gene in HeLa nuclear extracts.

Both Arabidopsis TATA binding protein (TBP) isoforms are functionally identical in RNA polymerase II and III transcription in plant cells: evidence for gene-specific changes in DNA binding specificity of TBP.

Contact Info

Product

Resources

About