Two-color SERS microscopy for protein co-localization in prostate tissue with primary antibody–protein A/G–gold nanocluster conjugates

Salehi, Mohammad; Schneider, Lilli; Ströbel, Philipp; Marx, Alexander; Packeisen, Jens; Schlücker, Sebastian

doi:10.1039/c3nr05890e

Cited by 52 publications

(52 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In practice, the conventional bioconjugation technique does not provide site-specificity and there is no control over targeting a particular amino acid residue. This uncontrolled binding in terms of missing site-specificity may result in a "scrambled" orientation of the antibodies on the surface of the SERS nanotags ( Figure Unauthenticated Download Date | 6/7/19 1:39 AM 2A) [13]. Thus the antigen recognition sites of the antibody (FAB) cannot recognize its target perfectly.…”

Section: Purity Of Antibodies and Missing Site-specificity Of Iggmentioning

confidence: 99%

“…Such stabilizers are able to bind with NPs covalently, like peptides or IgGs due to their free amine residues [9,13]. Figure 2B shows the competitive binding of IgGs and stabilizers with NP.…”

Section: Purity Of Antibodies and Missing Site-specificity Of Iggmentioning

confidence: 99%

“…50 nm, see Figure 2). Clusters of solid GNPs such as dimers and trimers exhibit a significantly larger SERS signal strength than individual solid GNPs or Au@Ag due to the presence of "hot spots" in the particle junctions ( Figure 1C) [12,13]. GNS and clusters of GNPs are just two optimal examples for highly sensitive plasmonic SERS nanotags, which have been characterized at the single-particle level [12,16].…”

Section: Highly Enhancing Sers Substrates For Ultrasensitive Moleculamentioning

confidence: 99%

“…Immuno-SERS-microscopy as a new methodology in immunohistochemistry (IHC) combines the high specificity of antigen-antibody interactions with the high sensitivity of SERS [10]. Particular features of SERS microscopy are the complexity of tissue as matrix and the dependence of target accuracy on several factors such as blocking buffers, antigen retrieval, incubation time and amount of nanotags [12,13]. Schlücker and co-workers established SERS microscopy as a method of microspectroscopic imaging for the selective detection of biomolecules in targeted research [10].…”

Section: Immuno-sers-microscopymentioning

confidence: 99%

“…2D, labeling of p63-proteins and PSA (2F) with nanoparticles, which were prepared in reaction/separation chamber. Negative control experiments in immuno-SERS microscopy using SERS nanotags conjugated to BSA (2E) [9,13].…”

Section: Highly Enhancing Sers Substrates For Ultrasensitive Moleculamentioning

confidence: 99%

See 4 more Smart Citations

Bioconjugation of SERS nanotags and increasing the reproducibility of results

Salehi¹,

Hamann-Steinmeier²

2018

Nano Online

Self Cite

View full text Add to dashboard Cite

Surface-enhanced Raman scattering (SERS) is a vibrational spectroscopy technique, which is used in the areas of medical diagnostics. This technique use the advantages of biofunctionalized nanoparticles (NPs) for imaging and quantifying of target molecules such as proteins in assays, cells and tissues. The lack of reliability and reproducibility of the results are major challenges in the application of diagnostics based of SERS substrates. The biofunction and success of nanomedical tasks depends on the quality of each involved element like antibodies (IgGs) and nanostructures before, during and after preparation or conjunction with nanoparticles. This short review summarizes current designs of different SERS substrates and highlights the improvement of particularly simple and gentle conjugation methods for targeting research with SERS labels.

show abstract

Section: Purity Of Antibodies and Missing Site-specificity Of Iggmentioning

confidence: 99%

Section: Purity Of Antibodies and Missing Site-specificity Of Iggmentioning

confidence: 99%

Section: Highly Enhancing Sers Substrates For Ultrasensitive Moleculamentioning

confidence: 99%

Section: Immuno-sers-microscopymentioning

confidence: 99%

Section: Highly Enhancing Sers Substrates For Ultrasensitive Moleculamentioning

confidence: 99%

See 3 more Smart Citations

Bioconjugation of SERS nanotags and increasing the reproducibility of results

Salehi¹,

Hamann-Steinmeier²

2018

Nano Online

Self Cite

View full text Add to dashboard Cite

show abstract

Silica‐Coated Plasmonic Metal Nanoparticles in Action

2018

View full text Add to dashboard Cite

Hybrid colloids consisting of noble metal cores and metal oxide shells have been under intense investigation for over two decades and have driven progress in diverse research lines including sensing, medicine, catalysis, and photovoltaics. Consequently, plasmonic core-shell particles have come to play a vital role in a plethora of applications. Here, an overview is provided of recent developments in the design and utilization of the most successful class of such hybrid materials, silica-coated plasmonic metal nanoparticles. Besides summarizing common simple approaches to silica shell growth, special emphasis is put on advanced synthesis routes that either overcome typical limitations of classical methods, such as stability issues and undefined silica porosity, or grant access to particularly sophisticated nanostructures. Hereby, a description is given, how different types of silica can be used to provide noble metal particles with specific functionalities. Finally, applications of such nanocomposites in ultrasensitive analyte detection, theranostics, catalysts, and thin-film solar cells are reviewed.

show abstract

Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Garcı́a

Mosquera

Plou

et al. 2018

Advanced Optical Materials

View full text Add to dashboard Cite

regulation of a variety of physiological and pathological processes, such as proliferation and adhesion, immune response, and cell differentiation. [8] The biological roles of carbohydrates as signaling effectors and recognition markers are associated to specific molecular recognition events in which proteins [9] or other carbohydrates [10] are involved. However, individual carbohydrate-based molecular interactions have been shown to be generally weak and, therefore, biomolecules usually present clusters of carbohydrates that interact cooperatively with their natural ligands (multivalent effect), thereby increasing the binding strength. [11] Interestingly, the multivalent carbohydrate presentation can be mimicked by man-made multivalent systems, which involve multiple synthetic oligosaccharides, [12][13][14][15][16][17] and have been used to address basic studies, but also as probe molecules and drugs. Mammalian cells are covered by a dense array of carbohydrates known as glycocalyx. The glycocalyx composition depends on the specific cellular state, and therefore it can provide information about diseases. For example, an increase of α-2,6-sialylation, fucosylation, and/or other tumor-associated carbohydrate antigens on the cellular membrane is considered as a biomarker of cancer progression, with diagnostic value. [18,19] In addition, oligosaccharides can be used as disease biomarkers for targeted therapy, and therefore the analysis and characterization of the composition of cellular glycans provide information of relevance to the diagnosis of several diseases. [20] Different strategies can be used to label glycans, including the use of covalent recognizing ligands (e.g., boronic acid), using specific carbohydrate-binding proteins, and via metabolic labeling.Plasmonics is based on the excitation of surface plasmons, that is, coherent oscillations of electrons induced by an electromagnetic radiation at metal-dielectric interfaces. [21] When the dimensions of plasmonic nanostructures are smaller than the wavelength of the incident light, the interaction between the electromagnetic field and surface charges results in nonpropagating oscillations denoted as localized surface plasmon resonances (LSPR). [22] LSPR frequencies in metal nanoparticles depend on their size, shape, organization, interparticle distance, and dielectric environment, ranging from the visible through the near-infrared (NIR). [23,24] A large volume of research has been devoted to the development of reliable methods for the preparation of plasmonic nanostructures with well-defined shape, size, and/or interparticle spacing, as basic components of various analytical applications. [25,26] Another important element, the surface chemistry of plasmonic The incorporation of nanomaterials in glycoscience research enables the design of highly selective and sensitive bioanalytical devices for the detection of disease-associated biomarkers. In particular, localized surface plasmon resonance (LSPR) and surface enhanced Raman scattering (SERS) spectroscopies ar...

show abstract

Two-color SERS microscopy for protein co-localization in prostate tissue with primary antibody–protein A/G–gold nanocluster conjugates

Cited by 52 publications

References 29 publications

Bioconjugation of SERS nanotags and increasing the reproducibility of results

Bioconjugation of SERS nanotags and increasing the reproducibility of results

Silica‐Coated Plasmonic Metal Nanoparticles in Action

Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Contact Info

Product

Resources

About