Two catalase homologs are involved in host protection against bacterial infection and oxidative stress in Crassostrea hongkongensis

Zhang, Yang; Fu, Di; Yu, Feng; Liu, Qiongyou; Yu, Ziniu

doi:10.1016/j.fsi.2011.08.005

Cited by 29 publications

(19 citation statements)

References 28 publications

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“…Here, two target genes, namely, catalase and GADD45, were also increased in oysters subjected to hypo-salinity conditions. Previous evidence showed that catalase can catalyze the decomposition of hydrogen peroxide, a detrimental ROS (reactive oxygen species) when produced in excess, and protect host cells against the oxidative injury in the Hong Kong oyster ( Zhang et al, 2011 ). GADD45 is another an oxidative stress response protein responsible for repairing oxidant induced DNA damage ( Li et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis

Xiao

Wong

et al. 2018

Front. Physiol.

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Crassostrea hongkongensis, a commercially valuable aquaculture species dwelling in estuaries along the coast of the South China Sea, is remarkable for its eurysalinity traits that enable its successful colonization of diverse osmotic niches ranging from near freshwater to seawater. In order to elucidate how this oyster copes with coastal waters with immense salinity differences, we performed in situ transcriptomic analysis (RNA-seq) to characterize the global expression patterns of oysters distributed across naturally formed salinity gradients in Zhenhai Bay along the northern coast of the South China Sea. Principal component analysis reveals distinct expression profiles of oysters living in the extreme conditions of hypo-salinity and hyper-salinity. Compared with the situation of optimal salinity for oyster growth, hypo-salinity mainly regulated expression of genes involved in FoxO and oxytocin signaling, tight junction and several immune pathways, while hyper-salinity altered gene expression implicated in amino acid metabolism, AMPK and PI3K-AKt signaling pathways, demonstrating the complexity and plasticity of transcriptomic expression underpinning oyster eurysalinity. Furthermore, the expression patterns of several genes correlated with salinity gradients reveals the fine-tuned coordination of molecular networks necessary for adaptive homeostasis in C. hongkongensis. In conclusion, a striking capacity and distinct patterns of transcriptomic expression contribute to eurysalinity adaptation in C. hongkongensis, which provides new mechanistic insights into the adaptive plasticity and resilience of marine mollusks.

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Section: Discussionmentioning

confidence: 99%

Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis

Xiao

Wong

et al. 2018

Front. Physiol.

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“…In this case, both genes were up-regulated (Table 3) with the A form showing a general up-regulation in response to all three chemicals, but with the B form, more specific to PHE. Catalytic activities of SOD and CAT have been identified as being involved in the cellular protection against ROS of molluscs (mussels and limpets) challenged with environmental pollutants and environmental stress (Ansaldo et al, 2005;Cheung et al, 2004;Lima et al, 2007;Zhang et al, 2011). However, some discrepancy between gene transcription and enzymatic activity of both antioxidants has recently been reported in Mytilus edulis challenged with chemical stressors (Giuliani et al, 2013).…”

Section: Detection Of Antioxidant Genes Of Interest For Ecotoxicologymentioning

confidence: 99%

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

et al. 2015

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Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana.Aquatic Toxicology http://dx.doi.org/10. 1016/j.aquatox.2015.06.012 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights-Thetranscriptome of the mangrove oyster Crassostrea brasilianawas sequenced.-The sequencing effort dramatically expanded the existing cDNA sequences available for the species.-Global analysis for transcription in the oyster treated with phenanthrene, diesel and domestic sewage was performed.-The pollutants altered mRNAs for genes in biotransformation, antioxidant and stress response pathways. Abstract 2The Brazilian oyster Crassostrea brasiliana was challenged to three common environmental contaminants: phenanthrene, diesel fuel water-accommodated fraction (WAF) and domestic sewage. Total RNA was extracted from the gill and digestive gland, and cDNA libraries were sequenced using the 454 FLX pla orm. The assembled transcriptome resulted in ˜20,000contigs, which were annotated to produce the first de novo transcriptome for C. brasiliana.Sequences were screened to identify genes potentially involved in the biotransformation of xenobiotics and associated antioxidant defence mechanisms. These gene families included those of the cytochrome P450 (CYP450), 70kDa heat shock, antioxidants, such as glutathione Stransferase, superoxide dismutase, catalase and also multi-drug resistance proteins. Analysis showed that the massive expansion of the CYP450 and HSP70 family due to gene duplication identified in the Crassostrea gigas genome also occurred in C. brasiliana, suggesting these processes form the base of the Crassostrea lineage. Preliminary expression analyses revealed several candidates biomarker genes that were up-regulated during each of the three treatments, suggesting the potential for environmental monitoring.

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“…Although several studies have produced EST sequences in C . hongkongensis [ 18 , 19 , 20 ], gene sequences for this species are still scarce in GenBank. Recently, high-throughput next-generation sequencing (NGS) technologies, which include Solexa/Illumina, SOLiD/Applied technologies and 454/Roche, have created unprecedented opportunities for generating genetic information in non-model species and have been utilized to survey the genes related to reproduction.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomics Analysis of Crassostrea hongkongensis for the Discovery of Reproduction-Related Genes

et al. 2015

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BackgroundThe reproductive mechanisms of mollusk species have been interesting targets in biological research because of the diverse reproductive strategies observed in this phylum. These species have also been studied for the development of fishery technologies in molluscan aquaculture. Although the molecular mechanisms underlying the reproductive process have been well studied in animal models, the relevant information from mollusks remains limited, particularly in species of great commercial interest. Crassostrea hongkongensis is the dominant oyster species that is distributed along the coast of the South China Sea and little genomic information on this species is available. Currently, high-throughput sequencing techniques have been widely used for investigating the basis of physiological processes and facilitating the establishment of adequate genetic selection programs.ResultsThe C.hongkongensis transcriptome included a total of 1,595,855 reads, which were generated by 454 sequencing and were assembled into 41,472 contigs using de novo methods. Contigs were clustered into 33,920 isotigs and further grouped into 22,829 isogroups. Approximately 77.6% of the isogroups were successfully annotated by the Nr database. More than 1,910 genes were identified as being related to reproduction. Some key genes involved in germline development, sex determination and differentiation were identified for the first time in C.hongkongensis (nanos, piwi, ATRX, FoxL2, β-catenin, etc.). Gene expression analysis indicated that vasa, nanos, piwi, ATRX, FoxL2, β-catenin and SRD5A1 were highly or specifically expressed in C.hongkongensis gonads. Additionally, 94,056 single nucleotide polymorphisms (SNPs) and 1,699 simple sequence repeats (SSRs) were compiled.ConclusionsOur study significantly increased C.hongkongensis genomic information based on transcriptomics analysis. The group of reproduction-related genes identified in the present study constitutes a new tool for research on bivalve reproduction processes. The large group of molecular markers discovered in this study will be useful for population screening and marker assisted selection programs in C.hongkongensis aquaculture.

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Two catalase homologs are involved in host protection against bacterial infection and oxidative stress in Crassostrea hongkongensis

Cited by 29 publications

References 28 publications

Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis

Analysis of in situ Transcriptomes Reveals Divergent Adaptive Response to Hyper- and Hypo-Salinity in the Hong Kong Oyster, Crassostrea hongkongensis

Key metabolic pathways involved in xenobiotic biotransformation and stress responses revealed by transcriptomics of the mangrove oyster Crassostrea brasiliana

Transcriptomics Analysis of Crassostrea hongkongensis for the Discovery of Reproduction-Related Genes

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