“Tuning aggregativeversusnon-aggregative lectin binding with glycosylated nanoparticles by the nature of the polymer ligand”

Abstract

Varying the nature of polymeric linkers modulates the aggregation response of glycosylated nanoparticles upon interaction with lectins.

The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

“…Poly( N -hydroxyethyl acrylamide), PHEA, was chosen as the polymer to give colloidally stable particles and as an acrylamide, it is not easily hydrolyzable unlike acrylates for example, Figure 2 B and Table 1 . 36 , 42 PHEA was synthesized using RAFT (reversible addition–fragmentation chain transfer) polymerization resulting in dispersities below 1.3. The PHEA lengths were selected on the basis of performance in initial lateral flow screening assays (data not shown) and from reports of their colloidal stability.…”

The SARS-COV-2 Spike Protein Binds Sialic Acids, and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device

“…The PHEA lengths were selected on the basis of performance in initial lateral flow screening assays (data not shown) and from reports of their colloidal stability. 36,42 Amino-glycans were synthesized by reduction of anomeric azides and subsequently conjugated to the PHEAs by displacement of the PFP group, which was confirmed by 19 F NMR. Polymers were then assembled onto citrate-stabilized gold nanoparticles and excess ligand removed by centrifugation/resuspension cycles.…”

“…Poly(N-hydroxyethyl acrylamide), PHEA, was chosen as the polymer to give colloidally stable particles and as an acrylamide, it is not easily hydrolyzable unlike acrylates for example, Figure 2B and Table 1. 36,42 PHEA was synthesized using RAFT (reversible addition−fragmentation chain transfer) polymerization resulting in dispersities below 1.3. The PHEA lengths were selected on the basis of performance in initial lateral flow screening assays (data not shown) and from reports of their colloidal stability.…”

Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands

“…1A). During a preliminary screen for BiGalHexNAcP donor specicity, we found Gal (9), 3FGal (10) and 6FGal (11) to be suitable donors, while little or no activity was displayed towards 2FGal (12) and 4FGal (13) in the one-pot, two enzyme system, when using GlcNAc-N 3 (14) as the acceptor (data not shown). Lacto-N-biose and uorinated derivatives were efficiently synthesized on semi-preparative scale using donors 9-11 and acceptors 5-8 & 14 ( Fig.…”

“…With this panel of uoro-glycan nanoparticles (GlycoAuNPs) in hand, their lectin binding affinity/selectively trends could be evaluated, initially using soybean agglutinin (SBA) which preferentially binds b-D-galactosides. 13,49 Binding was assessed by exploiting the optical properties of the GlycoAuNPs, whereby SBA binding leads to aggregation of the nanoparticles (Fig. 3A).…”