Tunable, Ultrasensitive pH‐Responsive Nanoparticles Targeting Specific Endocytic Organelles in Living Cells

Abstract

Umgebungseinfluss: Anpassbare, pH‐responsive Nanopartikel können selektiv in endozytischen Kompartimenten aktiviert werden. Bei hohen pH‐Werten bewirkt die Micellenbildung (links im Bild) eine Fluoreszenzlöschung durch Förster‐Energietransfer. Bei niedrigen pH‐Werten lösen sich die Micellen auf, was zu Fluoreszenz führt. Diese nichtlineare An/Aus‐Nanoplattform sollte für die diagnostische Bildgebung und den Wirkstofftransport interessant sein.

Facing the Design Challenges of Particle-Based Nanosensors for Metabolite Quantification in Living Cells

“…174 Another very interesting micelle-based sensor for measuring pH has been synthesized based on the selforganizing properties of the polymers, where the integrity of the micelle changes with pH, giving rise to a dequenching of the fluorophore signal that could then be monitored. 175,176 This principle was first reported by the authors in 2011 for probing early and late endosomes in cells and has later been shown to have interesting potential for in vivo detection of pH changes.…”

Hyperbranched–hyperbranched polymeric nanoassembly to mediate controllable co-delivery of siRNA and drug for synergistic tumor therapy

“…As stated above, the high trasfection activity of HBPO(OEI600-PBA) 10 was thought to associate with the weakened siRNA affity after the intracellular decomposition triggered by the remarkably lysosome acidity. [29] The western blot experiments further manifested the good transfection performance of HBPO(OEI600-PBA) 10 /Beclin1 siRNA in the transfected cells. In comparison, the negative control transfected with no-targeting NC-siRNA affected insignificantly the expression of Beclin 1 protein.…”

Light and temperature responsive block copolymer assemblies with tunable fluorescence emissions

“…Ultrapure water was added to adjust the polymer concentration for experiment. The dispersion was filtered through a 0.22 mm filter before use [26,27].…”