Tumor Necrosis Factor-α and Interleukin-1β Regulate Interleukin-8 Expression in Third Trimester Decidual Cells

Lockwood, Charles J.; Arcuri, Felice; Toti, Paolo; Felice, Claudio De; Krikun, Graciela; Guller, Seth; Buchwalder, Lynn; Schatz, Frederick

doi:10.2353/ajpath.2006.060185

Cited by 64 publications

(60 citation statements)

References 42 publications

(29 reference statements)

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“…4 free membranes, chorionic plate, and umbilical cord. Consistent with this acute inflammatory response, our laboratory 29 and that of Dudley 30 found that in cultured term DCs IL-1␤ enhances expression of well established neutrophil chemoattractants and activators such as IL-8, epithelial neutrophil-activating peptide (ENA-78), growthrelated oncogene-␣ (Gro-␣), and granulocyte-chemotactic protein-2 (MCP-2).…”

Section: Il-6 Regulation In Decidual Cells 1761mentioning

confidence: 58%

“…41 Moreover, inhibition of IL-6 expression by MPA in cultured term DCs described in the current study suggests a mechanism by which maintenance of high circulating progesterone levels blocks conversion of acute to chronic inflammation in the decidua. The specificity of this progestin-mediated inhibition of IL-6 expression is indicated by our observation that MPA did not affect expression of either GM-CSF, 40 M-CSF, 41 MCP-1, 42 or IL-8 29 by the DCs. The severe pathological complications associated with monocyte-associated chronic inflammation in the decidua described in the Alabama Preterm Birth Study were not experienced by the majority of patients with CAM in Figure 8.…”

Section: Il-6 Regulation In Decidual Cells 1761mentioning

confidence: 75%

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Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Lockwood

Murk

Kayisli

et al. 2010

The American Journal of Pathology

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Chorioamnionitis frequently precedes both genital tract and placental inflammation and is both a primary cause of maternal morbidity and a major antecedent of preterm premature rupture of the membranes (PPROM) as well as preterm delivery (PTD). In most cases of chorioamnionitis , neutrophils dominate the decidua. In a subset of these cases , a predominance of monocytes is uniquely associated with both neonatal intraventricular hemorrhage and death. The multifunctional cytokine , interleukin-6 , promotes local monocyte dominance via several mechanisms. In this study , immunostaining of placental sections revealed significantly higher interleukin-6 HSCOREs in decidual cells (DCs) but not in interstitial trophoblasts , in chorioamnionitis versus gestational agematched control placentas (P < 0.05). In confluent leukocyte-free term DCs , secreted interleukin-6 levels in incubations with estradiol-17␤ were increased 2500-fold by IL-1␤ (P < 0.05). This up-regulation was inhibited by more than 50% in parallel incubations that included medroxyprogesterone acetate (n ‫؍‬ 12, P < 0.05). Western blotting data confirmed these enzyme-linked immunosorbent assay results; quantitative RT-PCR findings demonstrated corresponding changes in interleukin-6 mRNA levels. Specific inhibitors of signaling for both nuclear factor-B activation and p38-mitogen-activated protein kinase , but not for protein kinase C , significantly decreased IL-1␤-enhanced interleukin-6 expression levels in cultured DCs. In conclusion , in situ and in vitro results indicate that significantly enhanced interleukin-6 expression levels in DCs during chorioamnionitis could be pivotal in skewing decidual monocyte differentiation to macrophages.

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Section: Il-6 Regulation In Decidual Cells 1761mentioning

confidence: 58%

Section: Il-6 Regulation In Decidual Cells 1761mentioning

confidence: 75%

Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Lockwood

Murk

Kayisli

et al. 2010

The American Journal of Pathology

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“…Bacterial-derived lipopolysaccharides enhance IL-1␤ and TNF-␣ expression in human decidual cells and decidual macrophages (9,10). Our laboratory found that both cytokines increased expression of neutrophil chemotactic and activating chemokines in term decidual cell monolayers (11). Neutrophils are the predominant immune cell type in the decidua during CAM and deciduitis.…”

mentioning

confidence: 79%

Progestin-Inflammatory Cytokine Interactions Affect Matrix Metalloproteinase-1 and -3 Expression in Term Decidual Cells: Implications for Treatment of Chorioamnionitis-Induced Preterm Delivery

Oner

Schatz

Kizilay

et al. 2008

The Journal of Clinical Endocrinology &Amp; Metabolism

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Context: Chorioamnionitis (CAM)-elicited preterm delivery (PTD) is associated with elevated amniotic fluid levels of IL-1␤ and TNF-␣. We hypothesized that IL-1␤ and TNF-␣ may induce matrix metalloproteinase (MMP)-1 and MMP-3 activity to promote PTD by degrading decidual and fetal membranes and cervical extracellular matrix.Objective: Our objective was to evaluate: 1) MMP-1 and MMP-3 expression in decidual sections from uncomplicated term, idiopathic preterm, and CAM-complicated deliveries, and 2) the separate and interactive effects of IL-1␤, TNF-␣, medroxyprogesterone acetate (MPA), and a p38 MAPK inhibitor (SB203580) on MMP-1 and MMP-3 expression in term decidual cells (DCs). Interventions and Main Outcome Measures:Decidua were immunostained for MMP-1 and MMP-3. Cultured term DCs were incubated with estradiol (E2) or E2 plus MPA with or without IL-1␤ or TNF-␣ with or without SB203580. ELISA and Western blotting assessed secreted MMP-1 and MMP-3 levels, quantitative real-time RT-PCR assessed mRNA levels, and substrate gel zymography was used to determined MMP-1 and MMP-3 proteolytic activity.Results: MMP-1 and MMP-3 immunostaining was more prominent in CAM-complicated decidua vs. control preterm and term decidual specimens (P Ͻ 0.05). Compared with basal outputs by DCs incubated with E2, TNF-␣ enhanced MMP-1 and MMP-3 secretion by 14 Ϯ 3-and 9 Ϯ 2-fold, respectively, and IL-1␤ increased MMP-1 and MMP-3 secretion by 13 Ϯ 3-and 19 Ϯ 2-fold, respectively (P Ͻ 0.05). Addition of MPA lowered basal MMP-1 and MMP-3 outputs by 70%, whereas the TNF-␣-and IL-1␤-enhanced MMP-1 and MMP-3 levels were blunted by more than 50% (P Ͻ 0.05). SB203580 suppressed TNF-␣-and IL-1␤-induced MMP-1 and MMP-3 secretion by severalfold. Western blotting confirmed the ELISA results, and mRNA levels corresponded with MMP-1 and MMP-3 protein levels. MMP-1 and MMP-3 proteolytic activity was confirmed by substrate gel zymography. Conclusion

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“…at ASPET Journals on May 11, 2018 dmd.aspetjournals.org chorioamnionitis (Lockwood et al, 2006) as well as in amniotic fluid and cord blood from women with intrauterine infection. We found a significant fold increase in IL-8 mRNA expression in placentas in PTLI, whereas there were no differences in placentas in preterm versus term pregnancy as demonstrated in comparisons between PTLNI and TL and TNL (Fig.…”

Section: Transporter Expression and Pregnancy Conditionmentioning

confidence: 99%

ATP-Binding Cassette Transporter Expression in Human Placenta as a Function of Pregnancy Condition

Mason

Buhimschi²,

Buhimschi³

et al. 2011

Drug Metab Dispos

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ABSTRACT:Fetal drug exposure is determined by the type and concentration of placental transporters, and their regulation is central to the development of new treatments and delivery strategies for pregnant women and their fetuses. We tested the expression of several clinically important transporters in the human placenta associated with various pregnancy conditions (i.e., labor, preeclampsia, and preterm labor-inflammation). Placentas were obtained from five groups of women at the time of primary cesarean section: 1) term no labor; 2) term labor; 3) preterm no labor (delivered for severe preeclampsia); 4) preterm labor without inflammation (PTLNI); and 5) preterm labor with inflammation (PTLI). Samples were analyzed by Western blot and immunohistochemistry to identify changes in protein expression. Relative mRNA expression was determined by quantitative real-time polymerase chain reaction. A functional genomic approach was used to identify placental gene expression and elucidate molecular events that underlie the given condition. Placental expression of ATP-binding cassette transporters from women in labor and women with preeclampsia was unaltered. Multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP) and mRNA expression increased in placentas of women with preterm labor with inflammation. Molecular pathways of genes up-regulated in PTLI samples included cytokinecytokine receptor interactions and inflammatory response compared with those in the PTLNI group. The mRNA expression of MDR1 and BCRP was correlated with that of interleukin-8, which also increased significantly in PTLI samples. These data suggest that the transfer of drugs across the placenta may be altered in preterm pregnancy conditions associated with inflammation through changes in MDR1 and BCRP.

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Tumor Necrosis Factor-α and Interleukin-1β Regulate Interleukin-8 Expression in Third Trimester Decidual Cells

Cited by 64 publications

References 42 publications

Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Regulation of Interleukin-6 Expression in Human Decidual Cells and Its Potential Role in Chorioamnionitis

Progestin-Inflammatory Cytokine Interactions Affect Matrix Metalloproteinase-1 and -3 Expression in Term Decidual Cells: Implications for Treatment of Chorioamnionitis-Induced Preterm Delivery

ATP-Binding Cassette Transporter Expression in Human Placenta as a Function of Pregnancy Condition

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