Tsoong induces apoptosis and inhibits proliferation, migration and invasion of pancreatic ductal adenocarcinoma cells

Abstract

The roots of Codonopsis cordifolioidea (classified as campanulaceae cordifolioidea), locally known as Tsoong, have been used as a tonic food. The major components isolated from Tsoong have been demonstrated to present anti-human immunodeficiency virus-1 activities and cytotoxicity against various tumor cell lines. However, the possible effects of the novel compound isolated from Tsoong, cordifoliketones A, on pancreatic ductal adenocarcinoma (PDAC) cells, are still unknown. In the present study, cordifoliketones A extractions were prepared from Tsoong, and the possible effects on PDAC cell growth, apoptosis, migration and invasion in vitro and in vivo were exlored. The cytotoxicity assay, apoptosis assay, western blotting, migration and invasion assay, and a PDAC cell (AsPC-1, BxPC-3 and PANC-1) xenograft mice model were employed. The results demonstrated that treatment with cordifoliketones A: i) inhibited proliferation and promoted apoptosis of PDAC cells; ii) significantly induced apoptosis and altered expression of apoptosis-associated proteins in a dose-dependent manner; iii) suppressed migration and invasion of PDAC cells in a dose-dependent manner; and iv) restrained the growth of PDAC neoplasm in nude mice. Furthermore, cordifoliketones A demonstrated non-cytotoxic activity in a panel of normal human cells, including hTERT-HPNE, 293, hepatocyte HL-7702 and HL-1 cells. Therefore, these data indicated that cordifoliketones A may be a potential candidate compound for the prevention of PDAC cell proliferation and metastasis, presumably by induction apoptosis and inhibiting viability, invasion and migration of PDAC cells.

Isoalantolactone inhibits pancreatic cancer proliferation by regulation of PI3K and Wnt signal pathway

“…The tumor growth was evaluated as the mean volume± SE. At the end of experiment, the IATL-mediated apoptosis in tumors was evaluated using flow cytometry [ 19 , 20 ].…”

Anticancer Properties of Lobetyolin, an Essential Component of Radix Codonopsis (Dangshen)

“…Another extract, from the roots of the plant Codonopsis bulleyana Forest ex diels (locally known as Tsoong), has been shown to display anticancer effects. This extract inhibited autophagy in cancer cells through activation of the NFκB pathway and induction of apoptosis, resulting in a dose-dependent reduction of HCT116 and SW480 colon cancer cell proliferation [ 49 , 50 ]. In addition, an oral administration of a high dose (20 g/kg) of the extract reduced the growth of SW480 tumor xenografts in mice.…”

The effect of Codonopis bulleynana Forest ex Diels on chronically constipated mice

“…Previous studies have found that Codonopsis bulleyana is a non-toxic food with multiple effects (Pinhua et al, 2015, Pinhua et al, 2013, Ruoli et al, 1999a, Ruoli et al, 1999b, Yunpeng et al, 2017, Zijun et al, 2009, Qiongfen et al, 2002, Luan et al, 2018a, Luan et al, 2018b, Qiongfen et al, 2003, Ruoli et al, 1999a, Ruoli et al, 1999b). In this experiment, we found that Codonopsis bulleyana can promote intestinal motility and improve the intestinal microbiota composition of the constipation model.…”

“…There is less research on disease treatment mechanism of Codonopsis bulleyana . Studies have shown that (Ruoli et al, 1999a, Ruoli et al, 1999b, Yunpeng et al, 2017, Zijun et al, 2009, Qiongfen et al, 2002, Luan et al, 2018a, Luan et al, 2018b, Shabi et al, 2018), Codonopsis bulleyana can improve intestinal function, regulate immune function, and enhance cardiovascular system, which can inhibit colorectal cancer in mice. Constipation can be treated by surgery, physical method, etc.…”