Trypsin Mediates Growth Phase-Dependent Transcriptional Regulation of Genes Involved in Biosynthesis of Ruminococcin A, a Lantibiotic Produced by a
            <i>Ruminococcus gnavus</i>
            Strain from a Human Intestinal Microbiota

Gómez, Alberto Gómez; Ladiré, M.; Marcille, F.; Fons, Michel

doi:10.1128/jb.184.1.18-28.2002

Cited by 48 publications

(72 citation statements)

References 53 publications

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“…As previously reported for some AMPs, including HD-5 (77,124) and some cathelicidins (212), it is interesting that two antibacterial substances produced by bacterial species in the intestinal microbiota, Peptostreptococcus sp. (325) and the R. gnavus E1 strain (78,126), require processing to be activated, after the proforms have been cleaved by trypsin.…”

Section: Barrier Effect Against Pathogensmentioning

confidence: 99%

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

2006

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SUMMARY The intestinal tract is a complex ecosystem that combines resident microbiota and the cells of various phenotypes with complex metabolic activities that line the epithelial wall. The intestinal cells that make up the epithelium provide physical and chemical barriers that protect the host against the unwanted intrusion of microorganisms that hijack the cellular molecules and signaling pathways of the host and become pathogenic. Some of the organisms making up the intestinal microbiota also have microbicidal effects that contribute to the barrier against enteric pathogens. This review describes the two cell lineages present in the intestinal epithelium: the goblet cells and the Paneth cells, both of which play a pivotal role in the first line of enteric defense by producing mucus and antimicrobial peptides, respectively. We also analyze recent insights into the intestinal microbiota and the mechanisms by which some resident species act as a barrier to enteric pathogens. Moreover, this review examines whether the cells producing mucins or antimicrobial peptides and the resident microbiota act in partnership and whether they function individually and/or synergistically to provide the host with an effective front line of defense against harmful enteric pathogens.

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Section: Barrier Effect Against Pathogensmentioning

confidence: 99%

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

2006

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“…1A). Transcription of rum operons has been shown to be induced in the presence of trypsin, probably through the two-component system RumRK (9).…”

mentioning

confidence: 99%

“…Lantibiotics are posttranslationally modified peptides characterized by the presence of lanthionines, ␤-methyllanthionines, and didehydrated amino acid residues (15). Recently, a 12.8-kb region from the genome of R. gnavus E1 containing the genetic determinants for RumA production has been characterized (9). The rumA gene cluster is organized in three operons with predicted functions in lantibiotic biosynthesis and secretion (rumA 1 A 2 A 3 MTX), signal-transduction regulation (rumRK), and producer self-immunity (rum-FEGHR2) (see Fig.…”

mentioning

confidence: 99%

Characterization of ISRgn 1 , a Novel Insertion Sequence of the IS 3 Family Isolated from a Bacteriocin-Negative Mutant of Ruminococcus gnavus E1

Gómez

Ladiré

Marcille

et al. 2002

Appl Environ Microbiol

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ISRgn1, an insertion sequence of the IS3 family, has been identified in the genome of a bacteriocin-negative mutant of Ruminococcus gnavus E1. The copy number of ISRgn1 in R. gnavus E1, as well as its distribution among phylogenetically E1-related strains, has been determined. Results obtained suggest that ISRgn1 is not indigenous to the R. gnavus phylogenetic group but that it can transpose in this bacterium.

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“…trypsyny. Takie szczepy do ujawnienia swojej funkcji wymagają dodatkowego przystosowania się do warunków panujących w jelitach (15). Jak już wspomniano, niektóre probiotyczne i jelitowe mikrobionty wytwarzają bakteriocyny i mikrocyny będące bakteriobójczymi lub bakteriostatycznymi substancjami o szerokim spektrum oddziaływania zarówno przeciwko bakteriom Gram-dodatnim, jak i Gram-ujemnym, w tym także drobnoustrojom pokrewnym (28).…”

Section: Kompetycyjne Wykluczanieunclassified

Mechanisms of maintenance of intestinal homeostasis by autochthonic microbiota and probiotics

Binek¹,

Kizerwetter-Świda²,

Cisek³

et al. 2016

Medycyna Weterynaryjna

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Intestinal microbes are taxonomically diverse and constitute an ecologically dynamic microbiom interactively performing various physiological and physiopathological processes. It has been proposed that normal intestinal microbiotas play a critical role in the host’s metabolic homeostasis and immune tolerance. The modulation of intestinal microbiota populations by prebiotics, probiotics, and synbiotics may be beneficial for the host’s health. Under certain conditions, the intestinal microbiota and the host’s homeostasis can be restored by introducing bacteria that co-mediate anti-inflammatory responses. Commensal microbes and probiotics exert their beneficial effect by at least three mechanisms. These include - the maintenance of the epithelial barrier function and the attenuation of changes in intestinal permeability through effects on tight junction, decreasing paracellular permeability, providing innate defense against pathogens, and enhancing the physical impediment of the mucous layer, - competitive exclusion by the application of probiotic bacteria stabilizing the indigenous microflora, - immunomodulatory capacity, affecting a variety of signaling pathways with modulation of proper immune, inflammatory and allergic responses. The epithelial gut barrier faces important challenges, since its function is to prevent pathogens and harmful elements of the gut lumen from penetrating into the internal environment. Competitive exclusion treatment can increase resistance to pathogen colonization and control intestinal disturbance. The dominance of symbiotic and probiotic bacteria among the gut microbiota favors a tolerogenic immune response. The release of secretory IgA stabilizes tight junctions between cells of the epithelial layer as well as hampers pathogens and symbionts invading deeper layers. The understanding of these vital processes may help to protect the host against infection, prevent chronic inflammation, and maintain mucosal integrity.

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Trypsin Mediates Growth Phase-Dependent Transcriptional Regulation of Genes Involved in Biosynthesis of Ruminococcin A, a Lantibiotic Produced by a Ruminococcus gnavus Strain from a Human Intestinal Microbiota

Cited by 48 publications

References 53 publications

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

The Front Line of Enteric Host Defense against Unwelcome Intrusion of Harmful Microorganisms: Mucins, Antimicrobial Peptides, and Microbiota

Characterization of ISRgn 1 , a Novel Insertion Sequence of the IS 3 Family Isolated from a Bacteriocin-Negative Mutant of Ruminococcus gnavus E1

Mechanisms of maintenance of intestinal homeostasis by autochthonic microbiota and probiotics

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