Trypanosoma cruzi RNA-binding protein ALBA30 aggregates into cytoplasmic foci under nutritional stress

Chame, Daniela Ferreira; Souza, Daniela de Laet; Vieira, Helaine Graziele Santos; Tahara, Erich Birelli; Macedo, Andréa Mara; Machado, Carlos Renato; Franco, Glória Regina

doi:10.1007/s00436-019-06554-w

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…In addition, experiments performed with the recombinant form of the protein (rTcALBA30) during the stationary phase of parasite growth also showed the recruitment of this protein to cytoplasmic foci. These data indicate that TcALBA30 aggregates into stress granules in parasites subjected to nutritional stress [88]. Taking into account that RNA‐binding proteins play an important role in T. gondii bradyzoite dormancy and that the formation of protein aggregates—aggresomes has an impact on the induction of dormancy in E. coli bacteria, we figured out if the cytoplasmic foci of protein and mRNA aggregates formed in T. cruzi cytoplasm under stress conditions would have any role during parasite dormancy.…”

Section: Protozoamentioning

confidence: 99%

Cellular dormancy: A widespread phenomenon that perpetuates infectious diseases

da Silva,

Velikkakam,

de Oliveira

et al. 2023

J Basic Microbiol

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Under adverse environmental conditions, microorganisms are able to enter a state of cellular dormancy which consists of cell cycle arrest and interruption of multiplication. This process ensures their perpetuation in the infected host organism and enables the spread of disease. Throughout biological evolution, dormancy allowed microorganisms to persist in a harsh niche until favorable conditions for their reactivation were re‐established. Here, we propose to discuss the dormancy of bacteria and protozoa pathogens focusing on the potential mechanisms and components associated with dormancy.

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Section: Protozoamentioning

confidence: 99%

Cellular dormancy: A widespread phenomenon that perpetuates infectious diseases

da Silva,

Velikkakam,

de Oliveira

et al. 2023

J Basic Microbiol

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“…All Tb ALBA proteins interact with translation machinery components and can be recruited to cytoplasmic starvation granules [ 119 , 120 ]. In T. cruzi, TcALBA30 is localized into cytoplasmic granules under starvation conditions [ 121 , 122 ]. As mentioned in the previous section, TcALBA30 controls the steady-state levels of beta-amastin mRNAs in epimastigotes through elements present in their 3′-UTR, and the TcALBA30 over-expression in epimastigotes, resulting in 50% decreased levels of amastin mRNAs compared to wild type parasites.…”

Section: The Importance Of Rnp Granules In Modulating Gene Expression...mentioning

confidence: 99%

Modulation of Virulence Factors during Trypanosoma cruzi Differentiation

et al. 2022

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Chagas disease is a neglected tropical disease caused by Trypanosoma cruzi. This protozoan developed several mechanisms to infect, propagate, and survive in different hosts. The specific expression of proteins is responsible for morphological and metabolic changes in different parasite stages along the parasite life cycle. The virulence strategies at the cellular and molecular levels consist of molecules responsible for mediating resistance mechanisms to oxidative damage, cellular invasion, and immune evasion, performed mainly by surface proteins. Since parasite surface coat remodeling is crucial to invasion and infectivity, surface proteins are essential virulence elements. Understanding the factors involved in these processes improves the knowledge of parasite pathogenesis. Genome sequencing has opened the door to high-throughput technologies, allowing us to obtain a deeper understanding of gene reprogramming along the parasite life cycle and identify critical molecules for survival. This review therefore focuses on proteins regulated during differentiation into infective forms considered virulence factors and addresses the current known mechanisms acting in the modulation of gene expression, emphasizing mRNA signals, regulatory factors, and protein complexes.

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