TRPM8, a Versatile Channel in Human Sperm

Blas, Gerardo A. De; Darszon, Alberto; Ocampo, Ana Y.; Serrano, Carmen; Castellano, Laura E.; Hernández‐González, Enrique O.; Chirinos, Mayel; Larrea, Fernando; Beltrán, Carmen; Treviño, Claudia L.

doi:10.1371/journal.pone.0006095

Cited by 88 publications

(81 citation statements)

References 49 publications

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“…These data extend the physiological characterization of TRPM8 in mouse sperm (25,27), and in doing so, we have described only the second endogenous protein antagonist of TRPM8 (37). TRPM8 is an outward rectifying cation channel of the TRP family and is permeable to Ca 2+ , Cs + , Na 2+ , and K + .…”

Section: E)supporting

confidence: 71%

“…These data raise the potential for CRISP4, via the regulation of ion channels, to impact upon several aspects of sperm function. In the present study, we chose to focus on the TRP channel subset of Ca 2+ conducting channels in mammalian sperm (25)(26)(27).…”

Section: Crisp4 Crisp Domain Can Inhibit Cationic Currents In Testicularmentioning

confidence: 99%

“…Several TRP channels have been localized to mouse or human sperm, including TRPC1-6 and TRPM8 (25,29,30). Given our recent data demonstrating the localization and functional analysis of TRMP8 on mouse sperm (27) and the considerable similarity of the current data to earlier investigations (26), we chose to analyze the affect of CRISP4 on TRPM8 in the first instance.…”

Section: Crisp4 Crisp Domain Can Inhibit Cationic Currents In Testicularmentioning

confidence: 99%

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Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Gibbs¹,

Orta

Reddy³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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110

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The cysteine-rich secretory proteins (CRISPs) are a group of four proteins in the mouse that are expressed abundantly in the male reproductive tract, and to a lesser extent in other tissues. Analysis of reptile CRISPs and mouse CRISP2 has shown that CRISPs can regulate cellular homeostasis via ion channels. With the exception of the ability of CRISP2 to regulate ryanodine receptors, the in vivo targets of mammalian CRISPs function are unknown. In this study, we have characterized the ion channel regulatory activity of epididymal CRISP4 using electrophysiology, cell assays, and mouse models. Through patch-clamping of testicular sperm, the CRISP4 CRISP domain was shown to inhibit the transient receptor potential (TRP) ion channel TRPM8. These data were confirmed using a stably transfected CHO cell line. TRPM8 is a major cold receptor in the body, but is found in other tissues, including the testis and on the tail and head of mouse and human sperm. Functional assays using sperm from wild-type mice showed that TRPM8 activation significantly reduced the number of sperm undergoing the progesteroneinduced acrosome reaction following capacitation, and that this response was reversed by the coaddition of CRISP4. In accordance, sperm from Crisp4 null mice had a compromised ability to undergo to the progesterone-induced acrosome reaction. Collectively, these data identify CRISP4 as an endogenous regulator of TRPM8 with a role in normal sperm function.cysteine-rich secretory proteins | antigen 5 | pathogenesis-related 1 | epididymal maturation | fertility

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Section: E)supporting

confidence: 71%

Section: Crisp4 Crisp Domain Can Inhibit Cationic Currents In Testicularmentioning

confidence: 99%

Section: Crisp4 Crisp Domain Can Inhibit Cationic Currents In Testicularmentioning

confidence: 99%

See 1 more Smart Citation

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Gibbs¹,

Orta

Reddy³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

110

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“…36 Transient receptor potential homologues have also been located in human sperm. 37,38 In addition, members of soluble Nethyl maleimide-sensitive factor attachment protein receptor proteins present in the acrosome region of mammalian sperm may also facilitate calcium entry, thereby leading to the AR. 39,40 The high intracellular free calcium concentration together with DAG leads to membrane fusion and finally acrosomal exocytosis.…”

Section: Signalling Events During Zp-mediated Acrosomal Exocytosismentioning

confidence: 99%

Acrosome reaction: relevance of zona pellucida glycoproteins

Gupta

Bhandari²

2010

Asian J Androl

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During mammalian fertilisation, the zona pellucida (ZP) matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction (AR) in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four (ZP1, ZP2, ZP3 and ZP4). ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans (in addition to ZP3), ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the G i -coupled receptor pathway, whereas ZP1-and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels (VOCCs), whereas ZP1-and ZP4-induced ARs involve both T-and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans (in addition to ZP3), ZP1 and ZP4 also participate in these stages of fertilisation. Keywords: acrosome reaction; fertilisation; oocyte; signalling pathways; spermatozoa; zona pellucida glycoproteins INTRODUCTION Mammalian fertilisation is a highly synchronized process that involves a complex series of interactions between the spermatozoon and the egg, culminating in their unison. The initial steps in fertilisation involves the binding of the spermatozoon to the zona pellucida (ZP) matrix surrounding the egg, followed by induction of the acrosome reaction (AR) in the zona-bound spermatozoon, a pre-requisite for penetration of the ZP matrix by the spermatozoon. The spermatozoon acrosome is a Golgi-derived organelle that forms a cap over the anterior two-thirds of its nucleus. The AR involves the fusion of the sperm membrane with the outer acrosomal membrane, resulting in release of the acrosomal contents and exposure of the inner acrosomal membrane on the anterior head of the spermatozoon. Various physiological agents, such as progesterone, serum albumin, follicular fluid, hormones (including biogenic amines), hydrolytic enzymes (particularly proteases), hyaluronic acid and ZP glycoproteins, have been implicated in the induction of the AR. [1][2][3] In the present paper, we review the role of the ZP matrix and its constituents in AR induction. Various downstream signalling pathways involved in the ZP glycoprotein-induced AR will also be discussed. On the basis of the current literature and studies from our group, the salient differences in the ZP glycoprotein-mediated induction of the AR in mouse versus human will be highlighted.

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“…Ionic currents with properties consistent with TRPM8 channels were recorded in testicular sperm Martinez-Lopez et al 2011 ). Intracellular Ca 2+ concentration [Ca 2+ ] i imaging experiments at different temperatures and membrane potential ( E m ) measurements suggested TRPM8-like channels are present in more mature mouse and human sperm (De Blas et al 2009 ;Martinez-Lopez et al 2011 ).…”

mentioning

confidence: 99%

Cl− Channels and Transporters in Sperm Physiology

Treviño

Orta

Figueiras-Fierro

et al. 2014

Sexual Reproduction in Animals and Plants

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Spermatozoa must decode environmental and cellular cues to succeed in fertilization, and this process relies heavily on ion channels. New observations bring to light the relevant participation of Cl − channels and anion transporters in some of the main sperm functions. Here we review the evidence that indicates the participation of Cl − channels in motility, maturation, and the acrosome reaction (AR), and what is known about their molecular identity and regulation. Our better understanding of sperm anion transport will yield tools to handle some infertility problems, improve animal breeding and preserve biodiversity, and develop selective and secure male contraceptives.

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TRPM8, a Versatile Channel in Human Sperm

Cited by 88 publications

References 49 publications

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Acrosome reaction: relevance of zona pellucida glycoproteins

Cl− Channels and Transporters in Sperm Physiology

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