Trolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats

Şeker, Uğur; Nergız, Yusuf; Aktaş, Ayfer; Akkuş, Murat; Özmen, Mehmet Ferit; Uyar, Emre; Söker, Sevda

doi:10.1016/j.jpurol.2020.05.008

Cited by 11 publications

(6 citation statements)

References 32 publications

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“…Most of the previously published articles examined antioxidant substances to suppress the adverse effects of I/R injury on testes (Erol et al, 2017). One of the most commonly observed changes in I/R injury is the accumulation of ROS, the up‐regulation of inflammatory cytokine release, germinal epithelial cell pyknosis, cellular desquamation, and the formation of multinucleated giant cells, which is thought to be evidence of a disruption in cell proliferation and maturity (Ayengin et al, 2021; Kohsaka et al, 2022; Seker et al, 2020). We observed severe pathological changes in testis, which were exposed to I/R injury as reported in literature.…”

Section: Discussionmentioning

confidence: 99%

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Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

et al. 2022

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Testicular ischaemia reperfusion (I/R) injury results with serious dysfunctions in testis. This study aims to explore effects of soluble guanylate cyclase (sGC) stimulator Riociguat on experimental testicular I/R injury in rats. Twenty‐one male rats were divided into three groups (Control, IR and IRR). The control group was not exposed to any application. Bilateral testis from IR and IRR animals were rotated 720° in opposite directions for 3 h to induce experimental testicular ischaemia. Animals in IR and IRR groups were subjected to 3 h of reperfusion. Isotonic and Riociguat were administered to the animals 30 min prior reperfusion by oral gavage. At the end of experiment, animals were sacrificed and tissue samples were used for analyses. Riociguat treatment significantly decreased tissue malondialdehyde and Luminol levels compared to the IR group (p < 0.05). The pathological changes, pro‐apoptotic proteins (Bax, Caspase 3, and Caspase 9) and apoptotic index in the IR group were down regulated in Riociguat treated animals (p < 0.05). Riociguat treatment was also significantly increased anti‐apoptotic Bcl‐2 expression, but alleviated tissue injury via modulating pro‐inflammatory cytokine IL‐1β levels and significantly (p < 0.05) down‐regulating NF‐κB activity. Moreover, mTOR and ERK phosphorylation increased in IR group (p < 0.05), but Riociguat treatment reduced protein phosphorylation. Our experiment indicated that targeting sGC might support surgical interventions in testicular I/R injury by modulating oxidative stress, inflammation, and apoptotic protein expression levels, but more detailed studies are required to explore the protective activity of Riociguat and underlying mechanisms in testicular I/R injury.

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Section: Discussionmentioning

confidence: 99%

“…Testicular apoptotic index (AI) in animals was obtained with TUNEL assay (Seker et al, 2020). For that purpose, testis sections were deparaffinized and rehydrated through an increasing alcohol series.…”

Section: Methodsmentioning

confidence: 99%

Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

et al. 2022

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“…Moreover, this treatment method could stimulate cell survival gene expression such as proliferating cell nuclear antigen (PCNA), Bcl-2, cAMP-responsive element modulatorτ. [56][57][58] The pro-apoptotic and apoptotic-related gene expression is reduced in the protective protocol as presented in Tables 1 and 2.…”

Section: Antioxidant Treatment In Tt and Td-induced Conditionsmentioning

confidence: 99%

“…These results are in agreement with different studies (Tables 1 and 2) suggesting that administration of antioxidant agents before TD surgery can protect the testis from I/R‐induced injuries in a dependent and /or independent of antioxidant enzyme activity manner. Moreover, this treatment method could stimulate cell survival gene expression such as proliferating cell nuclear antigen (PCNA), Bcl‐2, cAMP‐responsive element modulator‐τ 56–58 . The pro‐apoptotic and apoptotic‐related gene expression is reduced in the protective protocol as presented in Tables 1 and 2.…”

Section: Antioxidant Treatment In Tt and Td‐induced Conditionsmentioning

confidence: 99%

Testicular torsion in vivo models: Mechanisms and treatments

et al. 2023

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BackgroundTesticular torsion is a condition in which a testis rotates around its longitudinal axis and twists the spermatic cord. This in turn results in a significant decrease in blood flow and perfusion of testicular tissue. During Testicular torsion, the testicular tissue is affected by ischemia, heat stress, hypoxia, and oxidative and nitrosative stress. The testicular torsion should be considered an emergency condition and surgical intervention (testicular detorsion ) as the sole treatment option in viable cases involves counter‐rotation on twisted testes associated, when possible, to orchipexy, in order to avoid recurrence. Possible testicular detorsion side‐effects occur due to reperfusion and endothelial cells injury, microcirculation disturbances, and intense germ cells loss.ObjectivesTo discuss testicular torsion surgery‐based methods, different time frames for testicular torsion induction, and the associated pathophysiology by emphasizing cellular and molecular events as well as different therapeutic agent applications for testicular torsion.Materials and methodsWe reviewed all original research and epidemiological papers related to testicular torsion condition.ResultsTesticular torsion causes germ cell necrosis, arrested spermatogenesis, and diminished testosterone levels, with consequent infertility. Among different involved pathophysiological impacts, testicular torsion/detorsion‐induced ischemia seems to play the key role by leading the tissue toward other series of events in testis. Numerous studies have used adjuvant antioxidants, calcium channel blockers, anti‐inflammatory agents, or vasodilating agents in order to decrease these effects.Discussion and conclusionTo the best of our knowledge, no previously conducted study examined therapeutical agents' beneficial effects post clinical I/R condition in humans. Different agents targeting different pathophysiological conditions were used to ameliorate the ischemia/reperfusion‐induced condition in animal models, however, none of the administrated agents were tested in human cases. Although considering testicular detorsion surgery is still the golden method to reverse the testicular torsion condition and the surgical approach is undeniable, the evaluated agents with beneficial effects, need to be investigated furthermore in clinical conditions. Thus, furthermore clinical studies and case reports are required to approve the animal models proposed agents' beneficial impacts.

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“…Sections from each samples then stained with HE or PAS. HE staining was performed as described previously [23]. PAS staining performed with a ready to use staining kit (Cat no: 04-130802, Bio-optica MI, IT) and all steps were performed under the recommendations of the manufacturer's instructions.…”

Section: Histopathological Stainingmentioning

confidence: 99%

Nephroprotective Effect of Aloe Vera Extract With Regulation of Oxidative Stress, Apoptosis and Aquaporin 3 Expression Levels in Streptozotocin Induced Diabetic Rats

Şeker

Güzel

Şener

et al. 2023

Ankara Ecz. Fak. Derg.

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Objective: In this study we examined the protective activity of Aloe vera with considering anti-oxidant, anti-apoptotic properties, and the status of Aquaporin 3 (AQP3) channel protein. Material and Method: Twenty-one adult female rats were divided into three groups (n=7); Control, Diabetes, Treatment. Control group did not expose to any application. Animals in Diabetes and Treatment were exposed to experimental diabetes with administration of streptozotocin. Rats in Treatment received 300 mg/kg Aloe vera extract daily for 14 days. Rats were sacrificed and kidney samples were used for analyses. Result and Discussion: Analyses indicated that lowest malondialdehyde (MDA) and luminol levels in control group were increased significantly (p

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Trolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats

Cited by 11 publications

References 32 publications

Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

Targeting soluble guanylate cyclase with Riociguat has potency to alleviate testicular ischaemia reperfusion injury via regulating various cellular pathways

Testicular torsion in vivo models: Mechanisms and treatments

Nephroprotective Effect of Aloe Vera Extract With Regulation of Oxidative Stress, Apoptosis and Aquaporin 3 Expression Levels in Streptozotocin Induced Diabetic Rats

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