TRITIUM-LABELING BY EXPOSURE OF ORGANIC COMPOUNDS TO TRITIUM GAS<sup>1</sup>

Wilzbach, K. E.

doi:10.1021/ja01561a078

Cited by 628 publications

(102 citation statements)

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“…All of these patients had adrenal adenomas. In Patient 11 As in the patients with primary aldosteronism, the increased aldosterone production was observed in the presence of relatively high urine sodium levels ( Figure 1). The plasma bicarbonate levels were 28 mEq per L or higher in four of these patients, and in all but one plasma potassium levels were less than 4 mEq per L. With one exception (Patient 19) the increased aldosterone secretory rates were found in the patients with relatively high plasma bicarbonate levels.…”

Section: Resultsmentioning

confidence: 75%

Aldosterone Secretion and Primary and Malignant Hypertension *

Laragh¹,

Ulick²,

Januszewicz³

et al. 1960

J. Clin. Invest.

294

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Section: Resultsmentioning

confidence: 75%

Aldosterone Secretion and Primary and Malignant Hypertension *

Laragh¹,

Ulick²,

Januszewicz³

et al. 1960

J. Clin. Invest.

294

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“…Crystalline bilirubin was treated with tritium gas according to the procedure of Wilzbach (22). The crude product was washed with methanol and the methanol removed by evaporation.…”

Section: Methodsmentioning

confidence: 99%

Effect of sodium phenobarbital on bilirubin metabolism in an infant with congenital, nonhemolytic, unconjugated hyperbilirubinemia, and kernicterus

Crigler¹,

Gold²

1969

J. Clin. Invest.

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A B S T R A C T Sodium phenobarbital and various hormones, compounds capable of hepatic enzyme induction, were given to an infant boy with congenital, nonhemolytic, unconjugated, hyperbilirubinemia and severe kernicterus for prolonged periods between the ages of 2 and 25 months to determine their effect on serum bilirubin concentrations. Phenobarbital, 5 mg/day orally, on two occasions decreased serum bilirubin concentrations approximately threefold over a period of 30 days. Withdrawal of phenobarbital after the first study resulted in a gradual (30 days) return of serum bilirubin to pretreatment levels. The lower serum bilirubin concentrations observed when phenobarbital therapy was reinstituted were maintained for 61 days on 2.5 mg/kg per day of the drug. Orally administered L-triiodothyronine, 0.05-0.1 mg/day for 71 days, intramuscular human growth hormone, 1 mg/day for 21 days, and testosterone propionate, 0.1 mg/day for 9 days, did not decrease serum bilirubin levels below lowest control values of 18 mg/100 ml.Bilirubin-'H was administered twice before and once with bilirubin-"C during phenobarbital therapy to study the kinetics of bilirubin metabolism. Results of the first and second control studies and of the bilirubin-'H and bilirubin-"C phenobarbital studies, respectively, were as follows: tptal body bilirubin pools, 200, 184, 73, and 72 mg; half-lives, 111, 84, 37, and 39 hr; and turnover, 30, 37, 33, and 31 mg/day. The data show that the ap- tration and total body pool resulted from a comparable decrease in bilirubin half-life without a significant change in turnover.In vitro histological (electron microscopy) and enzymological studies of liver obtained by surgical biopsies before and during phenobaribtal administration showed that both the hepatocyte content of agranular endoplasmic reticulum (AER) and the ability of liver homogenate to conjugate p-nitrophenol were significantly increased during phenobarbital treatment.The observations suggest that phenobarbital affects bilirubin metabolism by the induction of an enzyme(s) with a slow rate(s) of degradation (or rapid rate of degradation with limited capacity).

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“…Steroid concentrations were determined by using the Zimmerman colorimetric technique (15). Solutions of radioactive etiocholanolone and androsterone of high specific activity, prepared by the Wilzbach exchange method (16), were niade in an identical manner, and aliquots were added to somie experimcaits in order to follow any small changes in the steroids during incubation.…”

Section: Methodsmentioning

confidence: 99%