Triploidy induction by heat-shock treatment in red tilapia

Pradeep, Padmaja Jayaprasad; Srijaya, Thekkeparambil Chandrabose; Bahuleyan, Aneesh; Renjithkumar, Chelapurath Radhakrishnan; Jose, Deepak; Papini, Alessio; Chatterji, Anil

doi:10.1080/00087114.2012.711678

Cited by 10 publications

(7 citation statements)

References 14 publications

(28 reference statements)

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“…The eggs used in the present study were relatively uniform with an average fertilization rate of 98%, but the hatching rate of triploid eggs (group) was lower (27%) compared to that of the diploid eggs (80%). Similar results were reported in other fish species, such as goldfish and red tilapia, which resulted in 13% and 36-69% hatching rates, respectively, post temperature shock (42 °C) for two min (Carman et al, 1991;Pradeep et al, 2012). The low hatching rate observed in the triploid is a consequence of the heat shock, which disturbed the embryogenesis process.…”

Section: Discussionsupporting

confidence: 72%

Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

Ibrahim¹,

Soelistyowati²,

Carman³

2017

Jurnal Akuakultur Indonesia

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This study was aimed to evaluate the growth performance and gonadal development of diploid and triploid striped catfish Pangasianodon hypophthalmus. Triploids were produced through a heat shock method at 42 °C for two min, at two min after fertilization. Before treatment performed, group of triploid and diploid were separated through nucleolus counting confirmed cromoseme counting. Five individual of each group at the age of nine months were tagged and reared for two months. Parameter of growth performance, feed conversion ratio, and survival rate were analysed using independent-samples t-test at confidence interval 95%, while gonadosomatic index (GSI) and gonad histology were analysed descriptively. No significant differences were observed between diploid and triploid fish in terms of growth performance, feed conversion ratio, and survival rate (P>0.05) during the two months rearing period, while GSI was higher in diploid (P<0.05) compared to triploid females. Histological observations of triploid female gonads showed early development stage, indicating sterility through symptoms such as oocytes degradation and abnormal development. Meanwhile, male gonad developed faster compared to female as spermatids were found in several lobules. As conclusion, diploid and triploid striped catfish P. hypophthalmus growth performance did not differ up to the age of 11 months, although some sterility symptoms were observed in both male and female.Keywords: diploid, triploid, growth, gonad, Pangasianodon hypophthalmus ABSTRAK Penelitian ini bertujuan untuk mengevaluasi performa pertumbuhan dan perkembangan gonad ikan patin siam Pangasianodon hypophthalmus diploid dan triploid. Triploid diproduksi dengan kejut suhu panas pada suhu 42 °C selama dua menit, pada dua menit setelah fertilisasi. Sebelum pengujian, kelompok diploid dan triploid dipisahkan dengan menganalisis jumlah nukleolus yang dikonfirmasi dengan penghitungan jumlah kromosom. Masing-masing lima ekor ikan diploid dan triploid pada umur sembilan bulan ditandai dan dipelihara selama dua bulan. Parameter pertumbuhan, rasio konversi pakan, dan kelangsungan hidup dianalisis menggunakan independent-samples t-test pada selang kepercayaan 95% sedangkan gonadosomatik indeks (GSI) dan histologi gonad dianalisis secara deskriptif. Pertumbuhan, rasio konversi pakan, dan kelangsungan hidup antara diploid dan triploid selama dua bulan pemeliharaan tidak menunjukkan perbedaan yang nyata (P>0,05). Nilai GSI diploid lebih tinggi (P<0,05) dibandingkan triploid, baik pada jantan maupun betina. Hasil histologi menunjukkan bahwa gonad ikan patin betina masih pada tahap perkembangan awal. Namun, indikasi steril terlihat dengan adanya degradasi oosit dan perkembangannya yang terganggu. Gonad jantan berkembang lebih cepat dibandingkan betina, dengan ditemukannya spermatid pada beberapa lobule. Gonad jantan triploid menunjukkan adanya gejala sterilitas tetapi tidak permanen, sebagian masih mampu berkembang hingga fase spermatid, namun jumlahnya lebih sedikit dibandingkan diploid. Be...

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Section: Discussionsupporting

confidence: 72%

Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

Ibrahim¹,

Soelistyowati²,

Carman³

2017

Jurnal Akuakultur Indonesia

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“…Varimax factor scatter plot of transformed morphometric data taken from triploid samples (n=100) and diploid fish samples (n=100) (Pradeep et al, 2011). Despite statistically significant differences observed in many morphological parameters measured, there was, however, no specific range or value that could be used to differentiate between triploid and diploid African catfish due to wide overlap.…”

Section: Figmentioning

confidence: 99%

Morphometric Variations Between Triploid and DiploidClarias gariepinus(Burchell, 1822)

Normala

Mohd

Abol

et al. 2017

Croatian Journal of Fisheries

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Several scientific methods have been described in the identification of triploid fish. However, many of these methods are not applicable for routine management purposes due to their complexity and cost. In this study, the possibility of using morphological variation as a least cost and less complex method of distinguishing triploid and diploid African catfish Clarias gariepinus (Burchell, 1822) was examined. Triploid catfish were produced by cold shock of fertilized eggs in 5°C for 20 mins (at approximately 3 mins after fertilization). The fish were incubated, hatched and raised for 3 months. Ploidy levels of the fish were then ascertained by observing the erythrocyte shape. Triploid erythrocyte was ellipsoidal in shape while diploid was round. Morphological characterization was then carried out on 100 samples each of triploid and diploid African catfish. Although significant differences were observed in many parameters, the principal morphometric difference between triploid and diploid African catfish could not be clearly distinguished. It was therefore concluded that morphological characteristics is not ideal for discriminating triploids and diploids of African catfish. The used of erythrocyte characteristics still remains the cheapest and relatively effective method for triploid and diploid determination in African catfish.

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“…We expected that the results would be the same for all species in the genus. Besides providing basic knowledge, cytogenetic studies have been successfully used for detection of ploidy in tilapias (genus Oreochromis), which were temperature shocked (Pradeep, Srijaya, Bahuleyan, et al 2012;). This technique is very important in hybridization programming of fishes or any other animals, as it can detect and correctly identify the induced diploid or triploid offspring, which may be further selected as maternal and/or paternal material for further breeding.…”

Section: Discussionmentioning

confidence: 99%

“…It follows that there are some variations of Epinephelus species, including E. malabalicus (Zou et al 2005), E. fuscoguttatus (Liao et al 2006;Wei et al 2009), E. moara (E. bruneus) (Guo et al 2006), and E. coioides (Wang et al 2010), showing a chromosome number of 48 with varied karyotypes. Cytogenetic studies provide important basic knowledge which can have applications for many other studies, such as for the detection of ploidy in fishes (Pradeep et al 2011;Pradeep, Srijaya, Bahuleyan, et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Karyotype analysis of two groupers,Epinephelusspecies (Serranidae)

et al. 2014

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This is an initial report of a cytogenetic study in two economically important species of Epinephelus, namely E. bleekeri and E. coeruleopunctatus, using the G-banding technique. Chromosome numbers of the two species are 2n = 48. The differences were found to be karyotype variations, with 48 telocentric chromosomes in E. bleekeri and two submetacentric and 46 telocentric chromosomes in E. coeruleopunctatus. Statistically, there were no karyotype variations between male and female individuals of each of the species. This basic knowledge is highly important for other technologies, such as those used in hybridization programming.

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Triploidy induction by heat-shock treatment in red tilapia

Cited by 10 publications

References 14 publications

Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

Morphometric Variations Between Triploid and DiploidClarias gariepinus(Burchell, 1822)

Karyotype analysis of two groupers,Epinephelusspecies (Serranidae)

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