Triple nanoemulsion potentiates the effects of topical treatments with microencapsulated retinol and modulates biological processes related to skin aging

Abstract

BACKGROUND The sum of environmental and genetic factors affects the appearance and function of the skin as it ages. The identification of molecular changes that take place during skin aging provides biomarkers and possible targets for therapeutic intervention. Retinoic acid in different formulations has emerged as an alternative to prevent and repair age-related skin damage. OBJECTIVES To understand the effects of different retinoid formulations on the expression of genes associated with biological processes that undergo changes during skin aging. METHODS Ex-vivo skin samples were treated topically with different retinoid formulations. The modulation of biological processes associated with skin aging was measured by Reverse Transcription quantitative PCR (RT-qPCR). RESULTS A formulation containing microencapsulated retinol and a blend of active ingredients prepared as a triple nanoemulsion provided the best results for the modulation of biological, process-related genes that are usually affected during skin aging. CONCLUSION This association proved to be therapeutically more effective than tretinoin or microencapsulated retinol used singly.

Biological activities of green coffee nanoemulsions evaluated through alternative methods: MTT, cellular proliferation, and HET-CAM assays

“…According to Afornali et al (2013), the results presented allow us to infer that the investigated nanostructured systems significantly increased the biological effects investigated due to the nanoencapsulation of green coffee oil. This fact can be confirmed through comparative analysis with the results of Voytena (2017), who used free green coffee oil, that is, not encapsulated, and found lower values of cell proliferation.…”

The use of retinol to correct age-related skin changes: a review of clinical effectiveness and safety

“…Многие из эффектов, в том числе ретинола, объясняются ре-зультатом их взаимодействия со специфическими рецепторами к ретиноевой кислоте и существова-нием ретиноид-связывающих белков (рис. 1) [14]. Так, при применении ретинола с антиоксидантами (витамины С, Е, цинк) было отмечено улучшение барьерной функции кожи и увеличение активности клеток иммунной системы [15,16].…”